Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis

doi:10.1186/s12886-023-02910-8

科研成果详情

题名	Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis
作者	Liu, Jiasheng; Zhu, Mengchao; Xu, Yitong; Zhang, Mengdi; Sun, Haisen; Wang, Yaqi; Yang, Qingwen; Li, Jin
发表日期	2023-04-20
发表期刊	BMC ophthalmology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Autophagy Human lens epithelial cells Single-cell RNA-sequencing Transmission electron microscopy
其他关键词	GENES
摘要	Autophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC subtypes have been identified, but autophagy-prominent cell clusters among HLECs have not been characterized., To explore the existence of autophagy-prominent cell clusters in HLECs., Three donated lenses (HLECs from two whole lenses and HLECs from one lens without the anterior central 6-mm zone) were used for single-cell RNA sequencing (scRNA-seq). AUCell and AddModuleScore analysis were used to identify potential autophagy-prominent cell clusters. Transmission electron microscopy (TEM) was used to confirm the results., High-quality transcripts from 18,120 cells were acquired by scRNA-seq of the two intact lenses. Unsupervised clustering classified the cells into four clusters. AUCell and AddModuleScore analysis revealed cluster 1 is autophagy-prominent. scRNA-seq analysis of HLECs from the lens capsule lacking the central zone confirmed the cluster 1 HLECs was located in the central capsule zone. The TEM result showed that greater autophagy activity was observed in the HLECs in central capsule zone, which further supported the above conclusions based on scRNA-seq analysis that autophagy was prominent in the central zone where the cluster 1 HLECs located., We identified an autophagy-prominent cell cluster among HLECs and revealed that it was localized in the central zone of the lens capsule. Our findings will aid investigations of autophagy in HLECs and provide insights to guide related research.
出版者	BMC
ISSN	1471-2415
EISSN	1471-2415
卷号	23 期号:1
DOI	10.1186/s12886-023-02910-8
页数	7
WOS类目	Ophthalmology
WOS研究方向	Ophthalmology
WOS记录号	WOS:000974110700003
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	37081480
SCOPUSEID	2-s2.0-85153447780
通讯作者地址	[Yang, Qingwen]Department of Cataract,Eye Hospital of Wenzhou Medical University,270# West Xueyuan Road, Zhejiang,Wenzhou,325000,China ; [Li, Jin]Department of Cataract,Eye Hospital of Wenzhou Medical University,270# West Xueyuan Road, Zhejiang,Wenzhou,325000,China
Scopus学科分类	Ophthalmology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/175138
专题	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者	Yang, Qingwen; Li, Jin
作者单位	Department of Cataract,Eye Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Liu, Jiasheng,Zhu, Mengchao,Xu, Yitong,et al. Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis[J]. BMC ophthalmology,2023,23(1).
APA	Liu, Jiasheng., Zhu, Mengchao., Xu, Yitong., Zhang, Mengdi., Sun, Haisen., ... & Li, Jin. (2023). Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis. BMC ophthalmology, 23(1).
MLA	Liu, Jiasheng,et al."Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis".BMC ophthalmology 23.1(2023).