Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice

doi:10.1186/s12906-023-03946-5

科研成果详情

题名	Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice
作者	Wang, Sisi1; Wu, Du 2; Wu, Fangquan1; Sun, Hongxia1; Wang, Xinyu1; Meng, Hongbing1; Lin, Qingqing 3; Jin, Keke1; Wang, Fangyan1
发表日期	2023-04-14
发表期刊	BMC COMPLEMENTARY MEDICINE AND THERAPIES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Prevotella histicola Ferroptosis Ethanol Gastric mucosal lesions System Xc- GPX4 axis ACSL4 VDAC
其他关键词	CELL-DEATH ; CANCER ; ULCER ; EXTRACT ; BARRIER ; LEAF
摘要	BackgroundEthanol-induced gastric mucosal lesions (EGML) is one of the most common digestive disorders for which current therapies have limited outcomes in clinical practice. Prevotella histicola (P. histicola) has shown probiotic efficacy against arthritis, multiple sclerosis and oestrogen deficiency-induced depression in mice; however, its role in EGML remains unclear in spite of its extensive colonisation of the stomach. Ferroptosis, which is characterised by lipid peroxidation, may be involved in EGML. Herein, we aimed to investigate the effects and underlying mechanism of action of P. histicola on EGML in the ferroptosis-dependent pathway.MethodsP. histicola was intragastrically administered for a week, and deferoxamine (DFO), a ferroptosis inhibitor, was intraperitoneally injected prior to oral ethanol administration. The gastric mucosal lesions and ferroptosis were assessed via histopathological examinations, quantitative real-time PCR, Western blot, immunohistochemistry and immunofluorescence.ResultsP. histicola was originally found to attenuate EGML by reducing histopathological changes and lipid reactive oxygen species (ROS) accumulation. The pro-ferroptotic genes of Transferrin Receptor (TFR1), Solute Carrier Family 39 Member 14 (SLC39A14), Haem Oxygenase-1 (HMOX-1), Acyl-CoA Synthetase Long-chain Family Member 4 (ACSL4), Cyclooxygenase 2 (COX-2) and mitochondrial Voltage-dependent Anion Channels (VDACs) were up-regulated; the anti-ferroptotic System Xc-/Glutathione Peroxidase 4 (GPX4) axis was inhibited after ethanol administration. However, the changes of histopathology and ferroptosis-related parameters induced by ethanol were reversed by DFO. Furthermore, P. histicola treatment significantly downregulated the expression of ACSL4, HMOX-1 and COX-2, as well as TFR1 and SLC39A14, on mRNA or the protein level, while activating the System Xc-/GPX4 axis.ConclusionsWe found that P. histicola reduces ferroptosis to attenuate EGML by inhibiting the ACSL4- and VDAC-dependent pro-ferroptotic pathways and activating the anti-ferroptotic System Xc-/GPX4 axis.
资助项目	Basic Public Welfare Research Project of Zhejiang Natural Science Foundation of China[LGF22H030011,LGF22H060014,LGF22H090028];Zhejiang Xiaolun Intelligent Manufacturing Co., Ltd.[KJHX2202];
出版者	BMC
ISSN	2662-7671
EISSN	2662-7671
卷号	23 期号:1
DOI	10.1186/s12906-023-03946-5
页数	12
WOS类目	Integrative & Complementary Medicine
WOS研究方向	Integrative & Complementary Medicine
WOS记录号	WOS:000968834100001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	37060026
SCOPUSEID	2-s2.0-85152589592
通讯作者地址	[Jin, Keke]School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China ; [Wang, Fangyan]School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Complementary and Alternative Medicine
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174872
专题	基础医学院（机能实验教学中心）附属第一医院
通讯作者	Jin, Keke; Wang, Fangyan
作者单位	1.School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China; 2.Hangzhou Wuyunshan Hospital Hangzhou Health Promotion Institution,Hangzhou,China; 3.Department of Hemodialysis,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Wang, Sisi,Wu, Du,Wu, Fangquan,et al. Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice[J]. BMC COMPLEMENTARY MEDICINE AND THERAPIES,2023,23(1).
APA	Wang, Sisi., Wu, Du., Wu, Fangquan., Sun, Hongxia., Wang, Xinyu., ... & Wang, Fangyan. (2023). Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice. BMC COMPLEMENTARY MEDICINE AND THERAPIES, 23(1).
MLA	Wang, Sisi,et al."Prevotella histicola suppresses ferroptosis to mitigate ethanol-induced gastric mucosal lesions in mice".BMC COMPLEMENTARY MEDICINE AND THERAPIES 23.1(2023).