Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans

doi:10.1111/cpr.13477

科研成果详情

题名	Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans
作者	Zhu, Meng-Chao1,2; Hu, Wei 3; Lin, Lei1,2; Yang, Qing-Wen1,2; Zhang, Lu1,2; Xu, Jia-Lin1,2; Xu, Yi-Tong1,2; Liu, Jia-Sheng1,2; Zhang, Meng-Di1,2; Tong, Xiao-Yu 4; Zhu, Kai-Yi1,2; Feng, Ke1,2; Feng, Yi 3; Su, Jian-Zhong1,2; Huang, Xiu-Feng4; Li, Jin1,2
发表日期	2023-04-14
发表期刊	CELL PROLIFERATION 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
其他关键词	TC1 C8ORF4 ; CATENIN ; LYMPHANGIOGENESIS ; IDENTIFICATION ; MECHANISMS ; EPITHELIUM ; ADAMTSL4 ; CD24 ; VEGF ; EYE
摘要	Although the cell atlas of the human ocular anterior segment of the human eye was revealed by single-nucleus RNA sequencing, whether subtypes of lens stem/progenitor cells exist among epithelial cells and the molecular characteristics of cell differentiation of the human lens remain unclear. Single-cell RNA sequencing is a powerful tool to analyse the heterogeneity of tissues at the single cell level, leading to a better understanding of the processes of cell differentiation. By profiling 18,596 cells in human lens superficial tissue through single-cell sequencing, we identified two subtypes of lens epithelial cells that specifically expressed C8orf4 and ADAMTSL4 with distinct spatial localization, a new type of fibre cells located directly adjacent to the epithelium, and a subpopulation of ADAMTSL4(+) cells that might be lens epithelial stem/progenitor cells. We also found two trajectories of lens epithelial cell differentiation and changes of some important genes during differentiation.
资助项目	National Natural Science Foundation of China [81670840]; Zhejiang Provincial Clinical Research Center for Pediatric Disease [2022E50003]
出版者	WILEY
ISSN	0960-7722
EISSN	1365-2184
卷号	56 期号:11
DOI	10.1111/cpr.13477
页数	14
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000970933700001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2023-04
URL	查看原文
PubMed ID	37057399
SCOPUSEID	2-s2.0-85152888300
通讯作者地址	[Su, Jian-Zhong]Institute of Biomedical Big Data,Wenzhou Medical University,270 West Xueyuan Road,Wenzhou,325027,China ; [Li, Jin]Eye Hospital of Wenzhou Medical University,270 West Xueyuan Road, Zhejiang,Wenzhou,325027,China ; [Feng, Yi]Department of Integrative Medicine and Neurobiology,State Key Lab of Medical Neurobiology,Institute of Integrative Medicine of Fudan University,Institute of Brain Science,School of Basic Medical Sciences,Fudan University,Shanghai,200032,China ; [Huang, Xiu-Feng]Zhejiang Provincial Clinical Research Center for Pediatric Disease,The Second Affiliated Hospital of Wenzhou Medical University,109 West Xueyuan Road, Zhejiang,Wenzhou,325027,China
Scopus学科分类	Cell Biology
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174863
专题	眼视光学院（生物医学工程学院）、附属眼视光医院附属第二医院第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Feng, Yi; Su, Jian-Zhong; Huang, Xiu-Feng; Li, Jin
作者单位	1.State Key Laboratory of Ophthalmology,Optometry and Visual Science,Eye Hospital,Wenzhou Medical University,Wenzhou,China; 2.National Clinical Research Center for Ocular Diseases,Eye Hospital,Wenzhou Medical University,Wenzhou,China; 3.Department of Integrative Medicine and Neurobiology,School of Basic Medical Sciences,Institutes of Brain Science,Brain Science Collaborative Innovation Center,State Key Laboratory of Medical Neurobiology,Institute of Acupuncture and Moxibustion,Fudan Institutes of Integrative Medicine,Fudan University,Shanghai,China; 4.Zhejiang Provincial Clinical Research Center for Pediatric Disease,The Second Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	温州医科大学; 眼视光学院（生物医学工程学院）、附属眼视光医院; 第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Zhu, Meng-Chao,Hu, Wei,Lin, Lei,et al. Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans[J]. CELL PROLIFERATION,2023,56(11).
APA	Zhu, Meng-Chao., Hu, Wei., Lin, Lei., Yang, Qing-Wen., Zhang, Lu., ... & Li, Jin. (2023). Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans. CELL PROLIFERATION, 56(11).
MLA	Zhu, Meng-Chao,et al."Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans".CELL PROLIFERATION 56.11(2023).