M6A-Related Long Non-Coding RNA Displays Utility in Predicting Prognosis, Portraying the Tumor Immune Microenvironment and Guiding Immunotherapy in Pancreatic Ductal Adenocarcinoma

doi:10.3390/vaccines11030499

科研成果详情

题名	M6A-Related Long Non-Coding RNA Displays Utility in Predicting Prognosis, Portraying the Tumor Immune Microenvironment and Guiding Immunotherapy in Pancreatic Ductal Adenocarcinoma
作者	Xu, Guangyu 1,2; Ji, Yutian 1,2; Wang, Lufeng 3; Xu, Hao 1,2; Shen, Chaodong4; Ye, Haihao 5; Yang, Xiangchou 1
发表日期	2023-03
发表期刊	VACCINES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	N6-methyladenosine lncRNA pancreatic ductal adenocarcinoma tumor immunology tumor immune microenvironment immunotherapy immune checkpoint blockade
其他关键词	CANCER ; FIBROBLASTS ; EXPRESSION ; FIBROSIS
摘要	N6-methyladenosine (m6A) lncRNA plays a pivotal role in cancer. However, little is known about its role in pancreatic ductal adenocarcinoma (PDAC) and its tumor immune microenvironment (TIME). Based on The Cancer Genome Atlas (TCGA) cohort, m6A-related lncRNAs (m6A-lncRNA) with prognostic value were filtered using Pearson analysis and univariate Cox regression analysis. Distinct m6A-lncRNA subtypes were divided using unsupervised consensus clustering. Least absolute shrinkage and selection operator (LASSO) Cox regression was applied to establish an m6A-lncRNA-based risk score signature. The CIBERSORT and ESTIMATE algorithms were employed to analyze the TIME. The expression pattern of TRAF3IP2-AS1 was examined using qRT-PCR. The influence of TRAF3IP2-AS1 knockdown on cell proliferation was estimated by performing CCK8, EdU and colony-formation assays. Flow cytometry was applied to measure the effect of TRAF3IP2-AS1 knockdown on cell cycle and apoptosis. The in vivo anti-tumor effect of TRAF3IP2-AS1 was validated in a tumor-bearing mouse model. Two m6A-lncRNA subtypes with different TIME features were clarified. A risk score signature was constructed as a prognostic predictor based on m6A-lncRNAs. The risk score also correlated with TIME characterization, which facilitated immunotherapy. Finally, the m6A-lncRNA TRAF3IP2-AS1 was proved to be a tumor suppressor in PDAC. We comprehensively demonstrated m6A-lncRNAs to be useful tools for prognosis prediction, TIME depiction and immunotherapeutic guidance in PDAC.
出版者	MDPI
ISSN	2076-393X
EISSN	2076-393X
卷号	11 期号:3
DOI	10.3390/vaccines11030499
页数	20
WOS类目	Immunology ; Medicine, Research & Experimental
WOS研究方向	Immunology ; Research & Experimental Medicine
WOS记录号	WOS:000958725800001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	36992083
SCOPUSEID	2-s2.0-85151948137
通讯作者地址	[Yang, Xiangchou]Department of Hematology and Medical Oncology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Immunology;Pharmacology;Drug Discovery;Infectious Diseases;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174824
专题	第二临床医学院，附属第二医院、育英儿童医院附属第一医院
通讯作者	Yang, Xiangchou
作者单位	1.Department of Hematology and Medical Oncology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Zhejiang University School of Medicine,Hangzhou,310030,China; 3.Neurology Department,East Hospital,Tongji University,Shanghai,310000,China; 4.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 5.Department of Cardiology,Wenzhou Traditional Chinese Medicine Hospital,Wenzhou,325000,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Xu, Guangyu,Ji, Yutian,Wang, Lufeng,et al. M6A-Related Long Non-Coding RNA Displays Utility in Predicting Prognosis, Portraying the Tumor Immune Microenvironment and Guiding Immunotherapy in Pancreatic Ductal Adenocarcinoma[J]. VACCINES,2023,11(3).
APA	Xu, Guangyu., Ji, Yutian., Wang, Lufeng., Xu, Hao., Shen, Chaodong., ... & Yang, Xiangchou. (2023). M6A-Related Long Non-Coding RNA Displays Utility in Predicting Prognosis, Portraying the Tumor Immune Microenvironment and Guiding Immunotherapy in Pancreatic Ductal Adenocarcinoma. VACCINES, 11(3).
MLA	Xu, Guangyu,et al."M6A-Related Long Non-Coding RNA Displays Utility in Predicting Prognosis, Portraying the Tumor Immune Microenvironment and Guiding Immunotherapy in Pancreatic Ductal Adenocarcinoma".VACCINES 11.3(2023).