Salicylate-Induced Hearing Loss Trigger Structural Synaptic Modifications in the Ventral Cochlear Nucleus of Rats via Medial Olivocochlear (MOC) Feedback Circuit

doi:10.1007/s11064-016-1836-x

科研成果详情

题名	Salicylate-Induced Hearing Loss Trigger Structural Synaptic Modifications in the Ventral Cochlear Nucleus of Rats via Medial Olivocochlear (MOC) Feedback Circuit
作者	Fang, Lian1,2; Fu, YaoYao 1; Zhang, Tian-yu 1
发表日期	2016-06
发表期刊	NEUROCHEMICAL RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Ventral cochlear nucleus Auditory system Tinnitus Synapses Growth associated protein-43
其他关键词	NECROSIS-FACTOR-ALPHA ; INFERIOR COLLICULUS ; INDUCED TINNITUS ; ACOUSTIC TRAUMA ; NEURAL ACTIVITY ; UP-REGULATION ; EXPRESSION ; GAP-43 ; BRAIN ; PLASTICITY
摘要	Lesion-induced cochlear damage can result in synaptic outgrowth in the ventral cochlear nucleus (VCN). Tinnitus may be associated with the synaptic outgrowth and hyperactivity in the VCN. However, it remains unclear how hearing loss triggers structural synaptic modifications in the VCN of rats subjected to salicylate-induced tinnitus. To address this issue, we evaluated tinnitus-like behavior in rats after salicylate treatment and compared the amplitude of the distortion product evoked otoacoustic emission (DPOAE) and auditory brainstem response (ABR) between control and treated rats. Moreover, we observed the changes in the synaptic ultrastructure and in the expression levels of growth-associated protein (GAP-43), brain-derived neurotrophic factor (BDNF), the microglial marker Iba-1 and glial fibrillary acidic protein (GFAP) in the VCN. After salicylate treatment (300 mg/kg/day for 4 and 8 days), analysis of the gap prepulse inhibition of the acoustic startle showed that the rats were experiencing tinnitus. The changes in the DPOAE and ABR amplitude indicated an improvement in cochlear sensitivity and a reduction in auditory input following salicylate treatment. The treated rats displayed more synaptic vesicles and longer postsynaptic density in the VCN than the control rats. We observed that the GAP-43 expression, predominantly from medial olivocochlear (MOC) neurons, was significantly up-regulated, and that BDNF- and Iba-1-immunoreactive cells were persistently decreased after salicylate administration. Furthermore, GFAP-immunoreactive astrocytes, which is associated with synaptic regrowth, was significantly increased in the treated groups. Our study revealed that reduced auditory nerve activity triggers synaptic outgrowth and hyperactivity in the VCN via a MOC neural feedback circuit. Structural synaptic modifications may be a reflexive process that compensates for the reduced auditory input after salicylate administration. However, massive increases in excitatory synapses in the VCN may represent a detrimental process that causes central hyperactivity, leading to tinnitus.
资助项目	National Natural Science Fund ProjectsNational Natural Science Foundation of China (NSFC) [81070786]; Zhejiang Natural Science Fund Projects [LY16H130001]; Shanghai Health System Talents Training Program [XBR2011068]; Shanghai Science and Technology CommitteeShanghai Science & Technology Committee [12XD1401700]
出版者	SPRINGER/PLENUM PUBLISHERS
出版地	NEW YORK
ISSN	0364-3190
EISSN	1573-6903
卷号	41 期号:6 页码:1343-1353
DOI	10.1007/s11064-016-1836-x
页数	11
WOS类目	Biochemistry & Molecular Biology ; Neurosciences
WOS研究方向	Biochemistry & Molecular Biology ; Neurosciences & Neurology
WOS记录号	WOS:000376076300015
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26886762
SCOPUSEID	2-s2.0-84958769884
通讯作者地址	[Zhang, Tian-yu]Department of Otolaryngology-Head and Neck Surgery,Shanghai Eye and ENT Hospital,Shanghai Medical College,Fudan University,83 Fenyang Road, Xuhui District,Shanghai,200031,China
Scopus学科分类	Biochemistry;Cellular and Molecular Neuroscience
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17464
专题	附属第一医院
通讯作者	Zhang, Tian-yu
作者单位	1.Department of Otolaryngology-Head and Neck Surgery,Shanghai Eye and ENT Hospital,Shanghai Medical College,Fudan University,83 Fenyang Road, Xuhui District,Shanghai,200031,China; 2.Department of Otolaryngology-Head and Neck Surgery,The First Affiliated Hospital of WenZhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院
推荐引用方式 GB/T 7714	Fang, Lian,Fu, YaoYao,Zhang, Tian-yu. Salicylate-Induced Hearing Loss Trigger Structural Synaptic Modifications in the Ventral Cochlear Nucleus of Rats via Medial Olivocochlear (MOC) Feedback Circuit[J]. NEUROCHEMICAL RESEARCH,2016,41(6):1343-1353.
APA	Fang, Lian, Fu, YaoYao, & Zhang, Tian-yu. (2016). Salicylate-Induced Hearing Loss Trigger Structural Synaptic Modifications in the Ventral Cochlear Nucleus of Rats via Medial Olivocochlear (MOC) Feedback Circuit. NEUROCHEMICAL RESEARCH, 41(6), 1343-1353.
MLA	Fang, Lian,et al."Salicylate-Induced Hearing Loss Trigger Structural Synaptic Modifications in the Ventral Cochlear Nucleus of Rats via Medial Olivocochlear (MOC) Feedback Circuit".NEUROCHEMICAL RESEARCH 41.6(2016):1343-1353.