题名 | Engineered exosomes derived from miR-132-overexpresssing adipose stem cells promoted diabetic wound healing and skin reconstruction |
作者 | |
发表日期 | 2023-03-01 |
发表期刊 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | diabetic wound miR-132 exosomes macrophages polarization inflammation |
其他关键词 | NF-KAPPA-B ; MICRORNA-132 ; ANGIOGENESIS |
摘要 | The tissue reconstruction of diabetic wounds mainly depends on the proliferation and remodelling of cutaneous cells around wounds and the transplantation of random skin flaps, however, the proliferation of cells or survival of skin flaps are difficult due to the severe inflammation and other problems caused by diabetes. The stem cell-derived exosomes loaded with miRNA can be an effective therapeutic strategy for promoting diabetic wound healing. Therefore, in this study, the engineered exosomes derived from miR-132-overexpressing adipose stem cells (miR-132-exo) was obtained for promoting the healing of diabetic wounds and skin flaps. In vitro, the miR-132-exo promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs). In vivo, streptozotocin (STZ) induced diabetic mice were used to create full-thickness skin wounds and random skin flaps to further investigate the healing effect of miR-132-exo. The results showed miR-132-exo evidently enhanced the survival of skin flaps and promote diabetic wound healing, through reducing local inflammation, promoting angiogenesis and stimulating M2-macrophages polarization mediated by NF-kappa B signaling pathway. These novel findings demonstrated that engineered miR-132-exo can be a potent therapeutic for treating diabetic wounds and inflammatory-related disease. |
资助项目 | Zhejiang Provincial Natural Science Foundation of China [Y19H060034] ; Medical Health Science and Technology Project of Zhejiang Province [2020KY183] ; National Undergraduate Training Programs for Innovation and Entrepreneurship [202110343034] |
出版者 | FRONTIERS MEDIA SA |
ISSN | 2296-4185 |
EISSN | 2296-4185 |
卷号 | 11 |
DOI | 10.3389/fbioe.2023.1129538 |
页数 | 18 |
WOS类目 | Biotechnology & Applied Microbiology ; Multidisciplinary Sciences |
WOS研究方向 | Biotechnology & Applied Microbiology ; Science & Technology - Other Topics |
WOS记录号 | WOS:000949382900001 |
收录类别 | SCIE ; EI ; PUBMED ; SCOPUS |
EI入藏号 | 20231213751600 |
EI主题词 | Polarization ; Polarization |
EI分类号 | 461.1 Biomedical Engineering ; 461.1 Biomedical Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.6 Medicine and Pharmacology ; 461.9 Biology ; 461.9 Biology ; 461.9.1 Immunology ; 461.9.1 Immunology |
URL | 查看原文 |
PubMed ID | 36937759 |
SCOPUSEID | 2-s2.0-85150274158 |
ESI高被引论文 | 2024-01 ; 2024-03 ; 2024-05 ; 2024-07 |
通讯作者地址 | [Mao, Cong]Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China ; [Feng, Yongzeng]Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China |
Scopus学科分类 | Biotechnology;Bioengineering;Histology;Biomedical Engineering |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/174620 |
专题 | 第二临床医学院、附属第二医院、育英儿童医院 附属第二医院_骨科重点学科实验室 |
通讯作者 | Mao, Cong; Feng, Yongzeng |
作者单位 | 1.Department of Orthopaedics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 2.Zhejiang Provincial Key Laboratory of Orthopaedics,Wenzhou Medical University,Wenzhou,China |
第一作者单位 | 第二临床医学院,附属第二医院、育英儿童医院; 附属第二医院; 温州医科大学 |
通讯作者单位 | 第二临床医学院,附属第二医院、育英儿童医院; 附属第二医院 |
第一作者的第一单位 | 第二临床医学院,附属第二医院、育英儿童医院 |
推荐引用方式 GB/T 7714 | Ge, Lifeng,Wang, Kangyan,Lin, Hang,et al. Engineered exosomes derived from miR-132-overexpresssing adipose stem cells promoted diabetic wound healing and skin reconstruction[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2023,11. |
APA | Ge, Lifeng., Wang, Kangyan., Lin, Hang., Tao, Endong., Xia, Weijie., ... & Feng, Yongzeng. (2023). Engineered exosomes derived from miR-132-overexpresssing adipose stem cells promoted diabetic wound healing and skin reconstruction. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 11. |
MLA | Ge, Lifeng,et al."Engineered exosomes derived from miR-132-overexpresssing adipose stem cells promoted diabetic wound healing and skin reconstruction".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 11(2023). |
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