TESC promotes differentiated thyroid cancer development by activating ERK and weakening NIS and radioiodine uptake

doi:10.1007/s12020-023-03350-6

科研成果详情

题名	TESC promotes differentiated thyroid cancer development by activating ERK and weakening NIS and radioiodine uptake
作者	Guo, Yawen 1,2,3,4; Cai, Yefeng5,6; Song, Fahuan 1,2,3,4; Zhu, Lei 7; Hu, Yiqun 6; Liu, Yunye 1; Ma, Wenli 8; Ge, Jingyan 9; Zeng, Qian 9; Ding, Lingling 1; Li, Lebao 10; Zheng, Guowan 1,3,4; Ge, Minghua 1,3,4
发表日期	2023-09
发表期刊	Endocrine 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Differentiated thyroid cancer (RAIR-DTC) ERK1/2 Iodine resistance Na+/I−symporter (NIS) Radioiodine refractory Tescalcin (TESC)
其他关键词	BRAF ; EXPRESSION ; CARCINOMA ; TESCALCIN ; SYMPORTER ; BREAST
摘要	Most differentiated thyroid cancer (DTC) patients have a good prognosis after surgery, but radioiodine refractory differentiated thyroid cancer (RAIR-DTC) patients have a significantly reduced 5-year survival rate (<60%) and a significantly increased recurrence rate (>30%). This study aimed to clarify the tescalcin (TESC) role in promoting the malignant PTC progression and providing a potential target for RAIR-DTC treatment., We analyzed TESC expression and clinicopathological characteristics using the Cancer Genome Atlas (TCGA) and performed qRT-PCR on tissue samples. TPC-1 and IHH-4 proliferation, migration, and invasion were detected after transfection with TESC-RNAi. Using Western blot (WB), several EMT-related indicators were detected. Moreover, iodine uptake of TPC-1 and IHH-4 after transfection with TESC-RNAi was detected. Finally, NIS, ERK1/2, and p-ERK1/2 levels were determined by WB., TESC was significantly upregulated in DTC tissues and positively correlated with BRAF V600E mutation based on data analysis from TCGA and our center. Reduced expression of TESC in both IHH-4 (BRAF V600E mutation) and TPC-1 (BRAF V600E wild type) cells significantly inhibited cell proliferation, migration, and invasion. It downregulated the EMT pathway markers Vimentin and N-cadherin, and increased E- cadherin. Moreover, TESC knockdown significantly inhibited ERK1/2 phosphorylation and decreased NIS expression in DTC cells, with a remarkably increased iodine uptake rate., TESC was highly expressed in DTC tissues and may have promoted metastasis through EMT and induced iodine resistance by downregulating NIS in DTC cells.
资助项目	National Natural Science Foundation of China [82103199, 22177104]; Natural Science Foundation of Zhejiang Province [LY23H160024]; Medical Health Science and Technology Project of Zhejiang Provincial Health Commission [2021KY482, 2023RC001]; Traditional Chinese Medicine Science and Technology Project of Zhejiang Provincial Health Commission [2023ZL240]; Zhejiang Province Postdoctoral Research Excellence Funding Project [ZJ2021167]; First Affiliated Hospital of Wenzhou Medical University [FHY2019003]
出版者	SPRINGER
ISSN	1355-008X
EISSN	1559-0100
卷号	81 期号:3 页码:503-512
DOI	10.1007/s12020-023-03350-6
页数	10
WOS类目	Endocrinology & Metabolism
WOS研究方向	Endocrinology & Metabolism
WOS记录号	WOS:000963692800001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-04
URL	查看原文
PubMed ID	37020077
SCOPUSEID	2-s2.0-85151568284
通讯作者地址	[Zheng, Guowan]Otolaryngology& Head and Neck Center,Cancer Center,Department of Head and Neck Surgery,Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital,Hangzhou Medical College),Zhejiang,Hangzhou,310014,China ; [Ge, Minghua]Otolaryngology& Head and Neck Center,Cancer Center,Department of Head and Neck Surgery,Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital,Hangzhou Medical College),Zhejiang,Hangzhou,310014,China
Scopus学科分类	Endocrinology, Diabetes and Metabolism;Endocrinology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174261
专题	附属第一医院其他_附属第五医院（丽水市中心医院）
通讯作者	Zheng, Guowan; Ge, Minghua
作者单位	1.Otolaryngology& Head and Neck Center,Cancer Center,Department of Head and Neck Surgery,Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital,Hangzhou Medical College),Zhejiang,Hangzhou,310014,China; 2.Department of Public Health,Zhejiang University School of Medicine,Hangzhou,310014,China; 3.Key Laboratory of Endocrine Gland Diseases of Zhejiang Province,Hangzhou,310014,China; 4.Clinical Research Center for Cancer of Zhejiang Province,Zhejiang,Hangzhou,310014,China; 5.Department of Thyroid Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 6.Second Clinical Medical College,Zhejiang Chinese Medical University,Zhejiang Province,Hangzhou,310053,China; 7.Department of Thyroid Surgery,The Fifth Hospital Affiliated to Wenzhou Medical University,Lishui Central Hospital,Zhejiang Province,Lishui,323000,China; 8.Bengbu Medical College,Anhui,Bengbu,233030,China; 9.Key Laboratory of Bioorganic Synthesis of Zhejiang Province,College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou,310014,China; 10.School of Information Science and Engineering,Zhejiang Sci-Tech University,Hangzhou,310018,China
推荐引用方式 GB/T 7714	Guo, Yawen,Cai, Yefeng,Song, Fahuan,et al. TESC promotes differentiated thyroid cancer development by activating ERK and weakening NIS and radioiodine uptake[J]. Endocrine,2023,81(3):503-512.
APA	Guo, Yawen., Cai, Yefeng., Song, Fahuan., Zhu, Lei., Hu, Yiqun., ... & Ge, Minghua. (2023). TESC promotes differentiated thyroid cancer development by activating ERK and weakening NIS and radioiodine uptake. Endocrine, 81(3), 503-512.
MLA	Guo, Yawen,et al."TESC promotes differentiated thyroid cancer development by activating ERK and weakening NIS and radioiodine uptake".Endocrine 81.3(2023):503-512.