Molecular detection of Enterocytozoon bieneusi in farmed Asiatic brush-tailed porcupines (Atherurus macrourus) and bamboo rats (Rhizomys pruinosus) from Hainan Province, China: Common occurrence, wide genetic variation and high zoonotic potential

doi:10.1016/j.actatropica.2023.106915

科研成果详情

题名	Molecular detection of Enterocytozoon bieneusi in farmed Asiatic brush-tailed porcupines (Atherurus macrourus) and bamboo rats (Rhizomys pruinosus) from Hainan Province, China: Common occurrence, wide genetic variation and high zoonotic potential
作者	Zhao, Wei 1,2,3; Wang, Tingting 1; Ren, Guangxu 2,3,4; Li, Jiaqi 2,3,4; Tan, Feng1; Li, Wenting 5; Zhu, Chuanlong 5; Lu, Gang 2,3,4,5; Huang, Huicong1
发表日期	2023-06
发表期刊	Acta tropica 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Asiatic brush-tailed porcupine Bamboo rat Enterocytozoon bieneusi Genotype Zoonotic
其他关键词	GENOTYPES ; IDENTIFICATION ; MICROSPORIDIA ; INFECTION
摘要	We investigated the occurrence and genotypic diversity of E. bieneusi in farmed Asiatic brush-tailed porcupines and bamboo rats from Hainan Province, China. Four hundred and sixty-seven fresh feces were collected from 164 Asiatic brush-tailed porcupines and 303 bamboo rats. DNA extraction from the feces and genotyping of E. bieneusi were performed by the amplification of the internal transcribed spacer (ITS) region of rDNA of E. bieneusi using PCR. A neighbor-joining tree was constructed based on the sequences obtained here and other sequences of E. bieneusi genotypes stored in Genbank. The total rate of infection with E. bieneusi was 32.5% (152/467), with 14.6% (24/164) in Asiatic brush-tailed porcupines and 42.2% (128/303) in bamboo rats infected. Seventeen genotypes of E. bieneusi were identified including 12 known genotypes, i.e., D (n = 78), Henan-III (n = 21), SHW7 (n = 19), KIN-1 (n = 11), ETMK5 (n = 7), TypeIV (n = 4), EbpD (n = 2), EbpA (n = 1), EbpC (n = 1), S7 (n = 1), HNPL-III (n = 1), HNR-VII (n = 1), and five novel genotypes named as HNZS-I (n = 1) and HNHZ-I to HNHZ-IV (n = 1 per genotype). Phylogenetic analysis revealed that all the genotypes found here except genotype S7 fell into Group 1. The present study demonstrated a relatively high prevalence of E. bieneusi infection (32.5%) and a large genetic variation of E. bieneusi (seventeen genotypes) in farmed Asiatic brush-tailed porcupines and bamboo rats in Hainan, China. The high proportion (78.3%) of zoonotic genotypes identified in the animals investigated here suggests that there is the potential for zoonotic or cross-species transmission which may pose a serious public health threat in the area. Public education on the management of Asiatic brush-tailed porcupines and bamboo rats should be implemented in the investigated areas.
资助项目	Scientific Research Project of the Department of Education of Zhejiang Province [Y202249687]; Research Project of Hainan Academician Innovation Platform [YSPTZX202004]; National Natural Science Foundation of China [82060375, 81760378]; Major Science and Technology Program of Hainan Province [ZDKJ202003]; Hainan Talent Development Project [SRC200003]; Innovation Research Team Project of Hainan Natural Science Foundation [2018CXTD340]; Open Foundation of Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University [2020TTM004]; Hainan Province Clinical Medical Center
出版者	ELSEVIER
ISSN	0001-706X
EISSN	1873-6254
卷号	242
DOI	10.1016/j.actatropica.2023.106915
页数	9
WOS类目	Parasitology ; Tropical Medicine
WOS研究方向	Parasitology ; Tropical Medicine
WOS记录号	WOS:000969131200001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-03
URL	查看原文
PubMed ID	36997011
SCOPUSEID	2-s2.0-85151280226
通讯作者地址	[Lu, Gang]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,571199,China ; [Huang, Huicong]Department of Parasitology,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Parasitology;Infectious Diseases
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174193
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Lu, Gang; Huang, Huicong
作者单位	1.Department of Parasitology,Wenzhou Medical University,Wenzhou,325035,China; 2.Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,571199,China; 3.Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases,Hainan Medical University,Haikou,571199,China; 4.Department of Pathogenic Biology,Hainan Medical University,Haikou,571199,China; 5.Department of Tropical Diseases,The Second Affifiliated Hospital of Hainan Medical University,Haikou,570100,China
第一作者单位	医学寄生虫学
通讯作者单位	医学寄生虫学
第一作者的第一单位	医学寄生虫学
推荐引用方式 GB/T 7714	Zhao, Wei,Wang, Tingting,Ren, Guangxu,et al. Molecular detection of Enterocytozoon bieneusi in farmed Asiatic brush-tailed porcupines (Atherurus macrourus) and bamboo rats (Rhizomys pruinosus) from Hainan Province, China: Common occurrence, wide genetic variation and high zoonotic potential[J]. Acta tropica,2023,242.
APA	Zhao, Wei., Wang, Tingting., Ren, Guangxu., Li, Jiaqi., Tan, Feng., ... & Huang, Huicong. (2023). Molecular detection of Enterocytozoon bieneusi in farmed Asiatic brush-tailed porcupines (Atherurus macrourus) and bamboo rats (Rhizomys pruinosus) from Hainan Province, China: Common occurrence, wide genetic variation and high zoonotic potential. Acta tropica, 242.
MLA	Zhao, Wei,et al."Molecular detection of Enterocytozoon bieneusi in farmed Asiatic brush-tailed porcupines (Atherurus macrourus) and bamboo rats (Rhizomys pruinosus) from Hainan Province, China: Common occurrence, wide genetic variation and high zoonotic potential".Acta tropica 242(2023).