PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation

doi:10.3390/cancers15061801

科研成果详情

题名	PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation
作者	Chen, Xin1; Jiang, Mengying1; Zhou, Shengjie 2; Chen, Hong1; Song, Gendi1; Wu, Yichen1; Zhu, Xueqiong1,2
发表日期	2023-03
发表期刊	Cancers 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	PRAME Wnt/β-catenin signaling cervical cancer proliferation tumorigenesis
其他关键词	PREFERENTIALLY EXPRESSED ANTIGEN ; GENE-EXPRESSION ; MESENCHYMAL TRANSITION ; SIGNALING PATHWAY ; BETA-CATENIN ; MELANOMA ; METASTASIS ; BIOMARKERS ; PROGRESSION ; PROGNOSIS
摘要	A significant burden is placed on the lives of females due to cervical cancer, which is currently the leading cause of cancer death among women. Preferentially expressed antigen in melanoma (PRAME) belongs to the CTA gene family and was found to be abnormally expressed among different types of cancers. Our previous research also indicated that PRAME was highly expressed in cervical cancer compared with normal tissues. However, the roles and detailed mechanisms of PRAME have not been explored in cervical cancer. In the present study, the expression of PRAME in cervical tissues and cells was detected by immunohistochemistry (IHC), qRT-PCR, and Western blotting. Additionally, CCK-8, BrdU, scratch, transwell, and flow cytometry assays were conducted to explore the function of PRAME in regulating the malignant biological behaviors of cervical cancer cells. Nude mice were used to confirm the role of PRAME in tumor growth in vivo. Furthermore, the Wnt inhibitor MSAB was used to verify the role of PRAME in regulating the Wnt/β-catenin pathway both in vitro and in vivo. The results of IHC, qRT-PCR, and Western blotting showed that PRAME was highly expressed in cervical cancer tissues and cells. PRAME knockdown attenuated cell growth, migration, and invasion; induced G0/G1 arrest; and increased cell apoptosis in C33A and SiHa cells through Wnt/β-catenin signaling regulation. However, the upregulation of PRAME exhibited the opposite effects accordingly, which could be partly reversed via MSAB treatment. The growth rate of xenograft tumors was enhanced when PRAME was overexpressed via Wnt/β-catenin signaling activation. Taken together, PRAME is associated with cervical cancer occurrence and progression mediated by Wnt/β-catenin signaling, suggesting that PRAME might be a factor in manipulating cervical carcinogenesis and a potential therapeutic target.
资助项目	Natural Science Foundation of Zhejiang Province [LY21H160060]
出版者	MDPI
ISSN	2072-6694
EISSN	2072-6694
卷号	15 期号:6
DOI	10.3390/cancers15061801
页数	17
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000958351000001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36980687
SCOPUSEID	2-s2.0-85151338141
通讯作者地址	[Zhu, Xueqiong]Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Oncology;Cancer Research
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/174174
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院第一临床医学院（信息与工程学院）、附属第一医院_妇产科学
通讯作者	Zhu, Xueqiong
作者单位	1.Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,325027,China; 2.Department of Obstetrics and Gynecology,Taizhou Women and Children’s Hospital of Wenzhou Medical University,Taizhou,318000,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 妇产科
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Chen, Xin,Jiang, Mengying,Zhou, Shengjie,et al. PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation[J]. Cancers,2023,15(6).
APA	Chen, Xin., Jiang, Mengying., Zhou, Shengjie., Chen, Hong., Song, Gendi., ... & Zhu, Xueqiong. (2023). PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation. Cancers, 15(6).
MLA	Chen, Xin,et al."PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation".Cancers 15.6(2023).