Prevalence, genetic diversity and implications for public health of Enterocytozoon bieneusi in various rodents from Hainan Province, China

doi:10.1186/s13071-020-04314-9

科研成果详情

题名	Prevalence, genetic diversity and implications for public health of Enterocytozoon bieneusi in various rodents from Hainan Province, China
作者	Zhao, Wei1,2; Zhou, Huanhuan 2,3; Yang, Ling 1; Ma, Tianming 2,3; Zhou, Jingguo 2,3; Liu, Haiju 2,3; Lu, Gang 2,3,4; Huang, Huicong1
发表日期	2020-09-02
发表期刊	PARASITES & VECTORS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Enterocytozoon bieneusi Rodent Genotype ITS Zoonotic
其他关键词	CRYPTOSPORIDIUM SPP. ; GIARDIA-DUODENALIS ; GENOTYPES ; IDENTIFICATION ; INFECTION
摘要	Background Rodents, globally overpopulated, are an important source for zoonotic disease transmission to humans, includingEnterocytozoon bieneusi(one of the most prevalent zoonotic pathogens). Here, we studied the prevalence and performed genetic analyses ofE. bieneusiin rodents from the Hainan Province of China. Methods A total of 603 fresh fecal samples were gathered from 369 wild rats, 117 bamboo rats, 93 Asiatic brush-tailed porcupine and 24 red-bellied squirrels. The wild rats were identified to the species level by amplification of a 421-bp region of thecytbgene from fecal DNA using PCR. Genotype analysis was performed by amplification of the internal transcribed spacer (ITS) region of rDNA ofE. bieneusiusing PCR. Results Seven wild rat species were identified. The average rate of infection withE. bieneusiwas 15.8% (95/603) with 18.7% (69/369) in wild rats, 11.9% (25/210) in farmed rodents and 4.2% (1/24) in red-bellied squirrels. SixteenE. bieneusigenotypes were identified, including 9 known genotypes (D, Type IV, PigEBITS7, Peru8, Peru11, ESH02, S7, EbpA and CHG5), and 7 novel genotypes (HNR-I to HNR-VII). Genotype D (44.2%, 42/95) predominated, followed by PigEBITS7 (20.0%, 19/95), HNR-VII (15.8%, 15/95), Type IV (5.3%, 5/95), HNR-III (2.1%, 2/95), HNR-VI (2.1%, 2/95) and each of the remaining 10 genotypes (1.1%, 1/95). The phylogenetic analysis of the ITS region ofE. bieneusidivided the identified genotypes into the following four groups: Group 1 (n= 13), Group 2 (n= 1), Group 12 (n= 1), and the novel Group 13 (n= 1). Conclusions To our knowledge, this is the first report on the identification ofE. bieneusiin rodents from Hainan, China. The zoonotic potential of the identifiedE. bieneusigenotypes suggested that the rodents poses a serious threat to the local inhabitants. Thus, measures need to be taken to control the population of wild rats in the areas investigated in this study, along with identification of safe methods for disposal of farmed rodent feces. Additionally, the local people should be made aware of the risk of disease transmission from rodents to humans.
资助项目	Innovation Research Team Project of Hainan Natural Science Foundation [2018CXTD340]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81672072, 81760378]
出版者	BMC
出版地	LONDON
ISSN	1756-3305
卷号	13 期号:1 页码:438
DOI	10.1186/s13071-020-04314-9
页数	9
WOS类目	Parasitology ; Tropical Medicine
WOS研究方向	Parasitology ; Tropical Medicine
WOS记录号	WOS:000568391800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32878633
PMC记录号	PMC7466830
SCOPUSEID	2-s2.0-85090261117
通讯作者地址	[Huang, Huicong]Department of Parasitology,Wenzhou Medical University,Wenzhou,325035,China ; [Lu, Gang]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,571199,China
Scopus学科分类	Parasitology;Infectious Diseases
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17417
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Lu, Gang; Huang, Huicong
作者单位	1.Department of Parasitology,Wenzhou Medical University,Wenzhou,325035,China; 2.Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,571199,China; 3.Department of Pathogenic Biology,Hainan Medical University,Haikou,China; 4.Hainan Medical University,University of Hong Kong Joint Laboratory of Tropical Infectious Diseases,Haikou,China
第一作者单位	医学寄生虫学
通讯作者单位	医学寄生虫学
第一作者的第一单位	医学寄生虫学
推荐引用方式 GB/T 7714	Zhao, Wei,Zhou, Huanhuan,Yang, Ling,et al. Prevalence, genetic diversity and implications for public health of Enterocytozoon bieneusi in various rodents from Hainan Province, China[J]. PARASITES & VECTORS,2020,13(1):438.
APA	Zhao, Wei., Zhou, Huanhuan., Yang, Ling., Ma, Tianming., Zhou, Jingguo., ... & Huang, Huicong. (2020). Prevalence, genetic diversity and implications for public health of Enterocytozoon bieneusi in various rodents from Hainan Province, China. PARASITES & VECTORS, 13(1), 438.
MLA	Zhao, Wei,et al."Prevalence, genetic diversity and implications for public health of Enterocytozoon bieneusi in various rodents from Hainan Province, China".PARASITES & VECTORS 13.1(2020):438.