Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery

doi:10.1021/acs.analchem.3c00005

科研成果详情

题名	Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery
作者	Pan, Zhaoqi1,2; Zhu, Hui1,2; Zhang, Yibin 3; Liao, Qianling1; Sun, Yiping1; Wu, Ende1,2; Wang, Yanan1; Shi, Kesi 4; Zhang, Yikui1,2; Chen, Lu1; Ye, Mao 5; Wu, Wencan1,2
发表日期	2023-03-21
发表期刊	Analytical chemistry 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Article
关键词	Cells Controlled drug delivery Cytology Dermatology Diagnosis Oncology Targeted drug delivery Aptamers Bio-marker discovery Dna aptamer Early diagnosis Intraocular Liver metastasis Melanoma cells Molecular tools Targeted treatment Uveal melanoma
其他关键词	CELL-SELEX ; DACARBAZINE BOLD ; VINCRISTINE ; BLEOMYCIN ; DIAGNOSIS ; LOMUSTINE ; SELECTION ; SURVIVAL ; INSIGHT ; BIOLOGY
摘要	Uveal melanoma (UM) is the most common primary intraocular malignancy in adults. However, challenges in early diagnosis, high risk of liver metastasis, and lack of effective targeted therapy lead to poor prognosis and high mortality of UM. Therefore, generating an effective molecular tool for UM diagnosis and targeted treatment is of great significance. In this study, a UM-specific DNA aptamer, PZ-1, was successfully developed, which could specifically distinguish molecular differences between UM cells and noncancerous cells with nanomolar-range affinity and presented excellent recognition ability for UM in vivo and clinical UM tissues. Subsequently, the binding target of PZ-1 on UM cells was identified as JUP (junction plakoglobin) protein, which held great potential as a biomarker and therapeutic target for UM. Meanwhile, the strong stability and internalization capacity of PZ-1 were also determined, and a UM-specific aptamer-guided "nanoship" was engineered to load and selectively release doxorubicin (Dox) to targeted UM cells, with lower toxicity to nontumor cells. Taken together, the UM-specific aptamer PZ-1 could serve as a molecular tool to discover the potential biomarker for UM and to achieve the targeted therapy of UM.
资助项目	National Key R&D Program of China [2021YFA1101200]; National Natural Science Foundation of China [82171048]; Key R&D Program of Zhejiang Province [2019C03009, 2021C03065]; Key R&D Program of Wenzhou Eye Hospital [YNZD1201902]; Innovation Research Project of the Eye Hospital of Wenzhou Medical University [YNCX201603]
出版者	AMER CHEMICAL SOC
ISSN	0003-2700
EISSN	1520-6882
卷号	95 期号:11 页码:5095-5108
DOI	10.1021/acs.analchem.3c00005
页数	14
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:000936786900001
收录类别	PUBMED ; SCIE ; EI ; SCOPUS
在线发表日期	2023-02
EI入藏号	20230913647100
EI主题词	Biomarkers
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.6 Medicine and Pharmacology ; 461.9 Biology
URL	查看原文
PubMed ID	36812415
SCOPUSEID	2-s2.0-85148770169
自科自定义期刊分类	T3(B)类
通讯作者地址	[Wu, Wencan]The Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China ; [Ye, Mao]Molecular Science and Biomedicine Laboratory,State Key Laboratory for Chemo/Biosensing and Chemometrics,College of Biology,College of Chemistry and Chemical Engineering,Aptamer Engineering Center of Hunan Province,Hunan University,Hunan,Changsha,410082,China
Scopus学科分类	Analytical Chemistry
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172807
专题	眼视光学院（生物医学工程学院）、附属眼视光医院眼视光学院（生物医学工程学院）、附属眼视光医院_眼鼻相关及眼眶眼肿瘤专科
通讯作者	Ye, Mao; Wu, Wencan
作者单位	1.The Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Zhejiang,Wenzhou,325027,China; 2.Oujiang Laboratory,Zhejiang Lab for Regenerative Medicine,Vision and Brain Health,Zhejiang,Wenzhou,325000,China; 3.Department of Pathology,Xiangya Hospital,Central South University,Hunan,Changsha,410082,China; 4.The Second Affiliated Hospital of Zhejiang University,Zhejiang,Hangzhou,310000,China; 5.Molecular Science and Biomedicine Laboratory,State Key Laboratory for Chemo/Biosensing and Chemometrics,College of Biology,College of Chemistry and Chemical Engineering,Aptamer Engineering Center of Hunan Province,Hunan University,Hunan,Changsha,410082,China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Pan, Zhaoqi,Zhu, Hui,Zhang, Yibin,et al. Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery[J]. Analytical chemistry,2023,95(11):5095-5108.
APA	Pan, Zhaoqi., Zhu, Hui., Zhang, Yibin., Liao, Qianling., Sun, Yiping., ... & Wu, Wencan. (2023). Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery. Analytical chemistry, 95(11), 5095-5108.
MLA	Pan, Zhaoqi,et al."Development of Uveal Melanoma-Specific Aptamer for Potential Biomarker Discovery and Targeted Drug Delivery".Analytical chemistry 95.11(2023):5095-5108.