FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice via the BMP4-autophagy pathway

doi:10.3389/fphar.2022.1019755

科研成果详情

题名	FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice via the BMP4-autophagy pathway
作者	Kong, Xiaoxia1,2; Lu, Liling 2,3; Lin, Daopeng 2,4; Chong, Lei2; Wen, Shunhang2; Shi, Yaokai2; Lin, Lidan1; Zhou, Liqin 5; Zhang, Hongyu5,6; Zhang, Hailin2
发表日期	2022-12-22
发表期刊	Frontiers in pharmacology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	AECs BMP4 FGF10 acute lung injury autophagy
其他关键词	GROWTH-FACTOR ; AUTOPHAGY ; INFLAMMATION ; MECHANISMS ; MITOPHAGY ; CELLS ; BMP4
摘要	Introduction: Damage to alveolar epithelial cells caused by uncontrolled inflammation is considered to be the main pathophysiological change in acute lung injury. FGF10 plays an important role as a fibroblast growth factor in lung development and lung diseases, but its protective effect against acute lung injury is unclear. Therefore, this study aimed to investigate protective effect and mechanism of FGF10 on acute lung injury in mice. Methods: ALI was induced by intratracheal injection of LPS into 57BL/6J mice. Six hours later, lung bronchoalveolar lavage fluid (BALF) was acquired to analyse cells, protein and the determination of pro-inflammatory factor levels, and lung issues were collected for histologic examination and wet/dry (W/D) weight ratio analysis and blot analysis of protein expression. Results: We found that FGF10 can prevent the release of IL-6, TNF-α, and IL-1β, increase the expression of BMP4 and autophagy pathway, promote the regeneration of alveolar epithelial type Ⅱ cells, and improve acute lung injury. BMP4 gene knockdown decreased the protective effect of FGF10 on the lung tissue of mice. However, the activation of autophagy was reduced after BMP4 inhibition by Noggin. Additionally, the inhibition of autophagy by 3-MA also lowered the protective effect of FGF10 on alveolar epithelial cells induced by LPS. Conclusions: These data suggest that the protective effect of FGF10 is related to the activation of autophagy and regeneration of alveolar epithelial cells in an LPS-induced ALI model, and that the activation of autophagy may depend on the increase in BMP4 expression
资助项目	New Drug Research and Development in the Major National Science and Technology Projects of China[2020ZX09201002];Wenzhou science and technology innovation project[ZY2020026];Ningbo Natural Science Foundation of China[2021J032,2022J043];National Science Foundation of China[81772450,81973382];
出版者	FRONTIERS MEDIA SA
ISSN	1663-9812
EISSN	1663-9812
卷号	13
DOI	10.3389/fphar.2022.1019755
页数	13
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000944087400001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36618911
SCOPUSEID	2-s2.0-85145577792
通讯作者地址	[Zhang, Hailin]Department of Children’s Respiration,The Second Affiliated Hospital & Yuying Children’s Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Zhang, Hongyu]Department of Pharmacy,Zhuji People’s Hospital,The Affiliated Hospital of Wenzhou Medical University,Zhejiang,Shaoxing,China
Scopus学科分类	Pharmacology;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172708
专题	基础医学院（机能实验教学中心）药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院其他_附属诸暨医院（诸暨市人民医院）其他_附属苍南医院（苍南县人民医院）其他_温州医科大学慈溪生物医药研究院
通讯作者	Zhang, Hongyu; Zhang, Hailin
作者单位	1.School of Basic Medical Sciences,Institute of Hypoxia Research,Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Department of Children’s Respiration,The Second Affiliated Hospital & Yuying Children’s Hospital,Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.Department of Ultrasound,Children’s Hospital,School of Medicine,Zhejiang University,Zhejiang,Hangzhou,China; 4.Department of Nephrology,Affiliated Cangnan Hospital,Wenzhou Medical University,Cangnan, Zhejiang,China; 5.Department of Pharmacy,Zhuji People’s Hospital,The Affiliated Hospital of Wenzhou Medical University,Zhejiang,Shaoxing,China; 6.School of Pharmaceutical Sciences,Cixi Biomedical Research Institute,Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	基础医学院（机能实验教学中心）; 附属第二医院
通讯作者单位	附属第二医院; 温州医科大学
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Kong, Xiaoxia,Lu, Liling,Lin, Daopeng,et al. FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice via the BMP4-autophagy pathway[J]. Frontiers in pharmacology,2022,13.
APA	Kong, Xiaoxia., Lu, Liling., Lin, Daopeng., Chong, Lei., Wen, Shunhang., ... & Zhang, Hailin. (2022). FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice via the BMP4-autophagy pathway. Frontiers in pharmacology, 13.
MLA	Kong, Xiaoxia,et al."FGF10 ameliorates lipopolysaccharide-induced acute lung injury in mice via the BMP4-autophagy pathway".Frontiers in pharmacology 13(2022).