Selective Expression of Huntingtin-associated Protein 1 in beta-Cells of the Rat Pancreatic Islets

doi:10.1369/jhc.2009.954479

科研成果详情

题名	Selective Expression of Huntingtin-associated Protein 1 in beta-Cells of the Rat Pancreatic Islets
作者	Liao, Min1,2; Chen, Xingxing 1; Han, Jinhong 1; Yang, Shiming 1; Peng, Ting 1; Li, He 1
发表日期	2010-03
发表期刊	JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	huntingtin-associated protein 1 insulin beta-cell pancreatic islet rat
其他关键词	INTRACELLULAR TRAFFICKING ; VESICULAR TRANSPORT ; HAP1 ; BRAIN ; GENE ; LOCALIZATION ; EXOCYTOSIS ; INTERACTS ; HOMOLOG ; NEURONS
摘要	Huntingtin-associated protein-1 (HAP1) was initially identified as a binding partner of huntingtin, the Huntington's disease protein. Based on its preferred distribution among neurons and endocrine cells, HAP1 has been suggested to play roles in vesicular transportation in neurons and hormonal secretion of endocrine cells. Given that HAP1 is selectively expressed in the islets of rat pancreas, in this study, we analyzed the expression pattern of HAP1 in the islets. In rats injected intraperitoneally with streptozotocin, which can selectively destroy beta-cells of the pancreatic islets, the number of HAP1 immunoreactive cells was dramatically decreased and was accompanied by a parallel decrease in the number of insulin-immunoreactive cells. Immunofluorescent double staining of pancreas sections showed that, in rat islets, HAP1 is selectively expressed in the insulin-immunoreactive beta-cells but not in the glucagon-immunoreactive alpha-cells and somatostatin immunoreactive delta-cells. In isolated rat pancreatic islets, similar to 80% of cells expressed both HAP1 and insulin. Expression of HAP1 in the INS-1 rat insulinoma cell line was also demonstrated by immunofluorescent staining. Western blotting further revealed that HAP1 in both the isolated rat pancreatic islets and the INS-1 cells also has two isoforms, HAP1A and HAP1B, which are the same as those in the hypothalamus. These results demonstrated that HAP1 is selectively expressed in beta-cells of rat pancreatic islets, suggesting the involvement of HAP1 in the regulation of cellular trafficking and secretion of insulin. (J Histochem Cytochem 58:255-263, 2010)
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30225024, 30671106]
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	0022-1554
EISSN	1551-5044
卷号	58 期号:3 页码:255-263
DOI	10.1369/jhc.2009.954479
页数	9
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000274601700006
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	19901268
SCOPUSEID	2-s2.0-77649258858
通讯作者地址	[Li, He]Department of Anatomy,Tongji Medical College,Huazhong University of Science and Technology,China
Scopus学科分类	Anatomy;Histology
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17244
专题	基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Li, He
作者单位	1.Department of Anatomy,Tongji Medical College,Huazhong University of Science and Technology,China; 2.Department of Histology and Embryology,Wenzhou Medical School,China
第一作者单位	组织胚胎学
推荐引用方式 GB/T 7714	Liao, Min,Chen, Xingxing,Han, Jinhong,et al. Selective Expression of Huntingtin-associated Protein 1 in beta-Cells of the Rat Pancreatic Islets[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY,2010,58(3):255-263.
APA	Liao, Min, Chen, Xingxing, Han, Jinhong, Yang, Shiming, Peng, Ting, & Li, He. (2010). Selective Expression of Huntingtin-associated Protein 1 in beta-Cells of the Rat Pancreatic Islets. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 58(3), 255-263.
MLA	Liao, Min,et al."Selective Expression of Huntingtin-associated Protein 1 in beta-Cells of the Rat Pancreatic Islets".JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 58.3(2010):255-263.