Overexpression of NT3P75-2 gene modified bone marrow mesenchymal stem cells supernatant promotes neurological function recovery in ICH rats

doi:10.1016/j.neulet.2023.137067

科研成果详情

题名	Overexpression of NT3P75-2 gene modified bone marrow mesenchymal stem cells supernatant promotes neurological function recovery in ICH rats
作者	Huang, Dongdong1,2; Bao, Han 1,2; Wu, Jian 1,2; Zhuge, Qichuan1,2; Yang, Jianjing1,2; Ye, Sheng1,2
发表日期	2023-02-06
发表期刊	Neuroscience letters 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Bone marrow mesenchymal stem cells Intracerebral hemorrhage M1/M2 microglia NT3(P75 - 2) Neurological function recovery
其他关键词	INTRACEREBRAL HEMORRHAGE ; NEURONAL DIFFERENTIATION ; TRANSPLANTATION ; PROLIFERATION ; NEUROTROPHIN ; STROKE ; GROWTH ; SURVIVAL ; BENEFITS ; DELIVERY
摘要	Intracerebral hemorrhage (ICH) is an acute cerebrovascular disease with high mortality and long-term disability rates. Stem cell transplantation and neurotrophic factor therapy have shown great potential in ICH. It has been established that mutated NT3 (NT3P75 - 2) can enhance the positive biological functions of NT3 by decreasing its affinity to the P75-2 receptor. The present study aimed to explore whether NT3P75-2 could further improve neurological recovery after ICH. First, we constructed three stable BMSC cell lines (GFP, GFP-NT3 overexpressed and GFP-NT3P75 - 2 overexpressed) by lentivirus infection. Next, rats were injected with fresh supernatants of these three cell lines on days 1 (24 h) and 3 (72 h) post-ICH induction. Behavioral evaluations were conducted to assess the neurological recovery of ICH rats. We further evaluated changes in microglia activation, neuron survival and proliferation of neural stem cells. Compared with the GFP group and the GFP-NT3 group, animals in the GFP-NT3P75 - 2 group exhibited better motor function improvements and milder neuroinflammation response. Meanwhile, overexpression of NT3P75 - 2 significantly decreased neuronal apoptosis and increased number of SOX2 - positive cells. Taken together, our study demonstrated that early administration of NT3P75 - 2 enriched BMMSC supernatants significantly enhanced neuro-functional recovery after ICH by regulating neuroinflammation response, neuronal survival and increasing neural stem cell number, providing a new therapeutic strategy and direction for early treatment of ICH
资助项目	International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China [81820108011]; National Natural Science Foundation of China [82103216]; Zhejiang Provincial Natural Sci-ence Foundation of China [LQ20H090005]
出版者	ELSEVIER IRELAND LTD
ISSN	0304-3940
EISSN	1872-7972
卷号	796
DOI	10.1016/j.neulet.2023.137067
页数	12
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000921037700001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-01
URL	查看原文
Pubmed记录号	36641043
Scopus记录号	2-s2.0-85146474078
通讯作者地址	[Yang, Jianjing]Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
scopus学科分类	Neuroscience (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172422
专题	第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室附属第一医院_神经外科
通讯作者	Yang, Jianjing
作者单位	1.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Neurosurgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, Dongdong,Bao, Han,Wu, Jian,et al. Overexpression of NT3P75-2 gene modified bone marrow mesenchymal stem cells supernatant promotes neurological function recovery in ICH rats[J]. Neuroscience letters,2023,796.
APA	Huang, Dongdong, Bao, Han, Wu, Jian, Zhuge, Qichuan, Yang, Jianjing, & Ye, Sheng. (2023). Overexpression of NT3P75-2 gene modified bone marrow mesenchymal stem cells supernatant promotes neurological function recovery in ICH rats. Neuroscience letters, 796.
MLA	Huang, Dongdong,et al."Overexpression of NT3P75-2 gene modified bone marrow mesenchymal stem cells supernatant promotes neurological function recovery in ICH rats".Neuroscience letters 796(2023).