Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR-188-3p/GRHL2 axis

doi:10.1111/aji.13657

科研成果详情

题名	Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR-188-3p/GRHL2 axis
作者	Zhou, Meixi 1; Yu, Xiang 2; Li, Chenping3; Lou, Lejing1; Yang, Song1; Cai, Jihao 4; Cai, Chang3
发表日期	2023-03
发表期刊	AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	circ_0111277 GRHL2 miR-188-3p PE trophoblast cells
其他关键词	CIRCULAR RNAS ; PREECLAMPSIA ; CANCER
摘要	ProblemPreeclampsia (PE) is the main factor threatening the life of primipara. Defective migration and invasion of trophoblast cells was one of the causes of PE. Circ_0111277 had been reported to be related to the development of PE, but the mechanism of its effect on trophoblast cells needed further study. Method of StudyThe expression of circ_0111277, microRNA-188-3p (miR-188-3p) and grainyhead-like 2 (GRHL2) mRNA were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) assay, 5-Ethynyl-20-deoxyuridine (EdU) and colony formation assay were used to examine cell proliferation ability. Tube formation and transwell assay were performed to assess the angiogenesis and metastasis ability of cells. Western blot was applied to measure the levels of epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin and Vimentin) and GRHL2 protein. The relationship between miR-188-3p and circ_0111277 or GRHL2 was verified by the dual luciferase reporter experiment. ResultsCirc_0111277 and GRHL2 were elevated, and miR-188-3p was declined in PE patients. Overexpression of circ_0111277 could inhibit the proliferation, angiogenesis, migration, invasion and EMT of trophoblast cells (HTR-8/Svneo). Circ_0111277 was the molecular sponge of miR-188-3p. MiR-188-3p up-regulation could reduce the inhibition of HTR-8/Svneo cell growth caused by overexpression of circ_0111277. GRHL2 was a target gene of miR-188-3p, and GRHL2 silencing relieved the adverse effects of miR-188-3p inhibitors on HTR-8/Svneo. In general, circ_0111277 up-regulated GRHL2 expression through sponge miR-188-3p. ConclusionHighly expressed circ_0111277 up-regulated the expression of GRHL2 through sponge miR-188-3p, thereby inhibiting trophoblast cells function, which suggested a new molecular mechanism for the pathogenesis of PE.
资助项目	ACKNOWLEDGEMENTS National Natural Science Foundation of China; [81470225]
出版者	WILEY
出版地	HOBOKEN
ISSN	1046-7408
EISSN	1600-0897
卷号	89 期号:3
DOI	10.1111/aji.13657
页数	11
WOS类目	Immunology ; Reproductive Biology
WOS研究方向	Immunology ; Reproductive Biology
WOS记录号	WOS:000891510500001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2022-11
URL	查看原文
PubMed ID	36409529
SCOPUSEID	2-s2.0-85142888422
通讯作者地址	[Cai, Chang]Shangcai Village, Nanbai Elephant Street, Ouhai District, Zhejiang, Wenzhou, 325000, China
Scopus学科分类	Immunology and Allergy;Immunology;Reproductive Medicine;Obstetrics and Gynecology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172089
专题	附属第一医院仁济学院第一临床医学院（信息与工程学院）、附属第一医院_内科学_呼吸与危重症医学科
通讯作者	Cai, Chang
作者单位	1.Department of Gynecology and Obstetrics,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of pulmonary and critical care medicine,Yongjia Hospital of traditional Chinese Medicine,Wenzhou,China; 3.Department of pulmonary and critical care medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Student Department,Renji College of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhou, Meixi,Yu, Xiang,Li, Chenping,et al. Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR-188-3p/GRHL2 axis[J]. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY,2023,89(3).
APA	Zhou, Meixi., Yu, Xiang., Li, Chenping., Lou, Lejing., Yang, Song., ... & Cai, Chang. (2023). Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR-188-3p/GRHL2 axis. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 89(3).
MLA	Zhou, Meixi,et al."Circ_0111277 suppresses trophoblast cell proliferation, angiogenesis, migration, invasion and EMT via regulating miR-188-3p/GRHL2 axis".AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY 89.3(2023).