Hsa_circ_0079480 enhances cell proliferation, migration, and invasion in colorectal cancer through miR-498/ATP5E axis

doi:10.1002/kjm2.12616

科研成果详情

题名	Hsa_circ_0079480 enhances cell proliferation, migration, and invasion in colorectal cancer through miR-498/ATP5E axis
作者	Lin, Ruo-Yang; Huang, Zhi-Ming
发表日期	2023-03
发表期刊	KAOHSIUNG JOURNAL OF MEDICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	ATP5E colorectal cancer hsa_circ_0079480 miR-498
其他关键词	IDENTIFICATION ; RNA
摘要	Circular RNAs play critical roles in tumorigenesis. hsa_circ_0079480 was reported to be upregulated in colorectal cancer (CRC). However, its specific molecule in CRC is poorly understood. Hsa_circ_0079480, miR-498, and ATP5E expressions in CRC tissues and CRC cells were determined using quantitative real-time polymerase chain reaction assay. ATP5E protein level was assessed using Western blot. Cell proliferation, migration, and invasion were examined by 3-(4, 5-Dimethylthiazolyl2)-2, 5-diphenyltetrazolium bromide assay and Transwell assays, respectively. Dual-luciferase reporter gene assay was performed to analyze the interactions between hsa_circ_0079480, miR-498, and ATP5E. This study results showed that hsa_circ_0079480 and ATP5E expressions were significantly increased in CRC tissues and CRC cells, while miR-498 was downregulated. Hsa_circ_0079480 knockdown dramatically suppressed CRC cell proliferation, migration, and invasion. Meanwhile, it turned out that hsa_circ_0079480 knockdown inhibited CRC tumor growth in vivo. Hsa_circ_0079480 could negatively regulate miR-498 expression by directly targeting miR-498. MiR-498 overexpression dramatically inhibited CRC cell malignant behaviors. miR-498 negatively regulated ATP5E expression by directly binding to ATP5E. ATP5E knockdown suppressed CRC cell malignant behaviors. ATP5E overexpression mitigated the inhibitory effect of hsa_circ_0079480 on CRC cell malignant behaviors. Since hsa_circ_0079480 knockdown inhibited CRC cells malignant behaviors through regulation of the miR-498/ATP5E axis, it can be concluded that hsa_circ_0079480 might have great potential as therapeutic target for CRC.
出版者	WILEY
出版地	HOBOKEN
ISSN	1607-551X
EISSN	2410-8650
卷号	39 期号:3 页码:209-220
DOI	10.1002/kjm2.12616
页数	12
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000911314300001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2023-01
URL	查看原文
PubMed ID	36625260
SCOPUSEID	2-s2.0-85146188163
通讯作者地址	[Huang, Zhi-Ming]Department of Gastroenterology and Hepatology,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Medicine (all)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172021
专题	附属第一医院附属第一医院_肝胆外科
通讯作者	Huang, Zhi-Ming
作者单位	Department of Gastroenterology and Hepatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Ruo-Yang,Huang, Zhi-Ming. Hsa_circ_0079480 enhances cell proliferation, migration, and invasion in colorectal cancer through miR-498/ATP5E axis[J]. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES,2023,39(3):209-220.
APA	Lin, Ruo-Yang, & Huang, Zhi-Ming. (2023). Hsa_circ_0079480 enhances cell proliferation, migration, and invasion in colorectal cancer through miR-498/ATP5E axis. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES, 39(3), 209-220.
MLA	Lin, Ruo-Yang,et al."Hsa_circ_0079480 enhances cell proliferation, migration, and invasion in colorectal cancer through miR-498/ATP5E axis".KAOHSIUNG JOURNAL OF MEDICAL SCIENCES 39.3(2023):209-220.