AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii

doi:10.3389/fmicb.2022.1035651

科研成果详情

题名	AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii
作者	Gao, Mengdi1,2,4; Feng, Chunlin2,4; Ji, Yongan1; Shi, Yaokai1; Shi, Weina2,4; Zhang, Lei2,4; Liu, Shuang2,4; Li, Anqi2,4; Zhang, Xueya1,2,4; Li, Qiaoling1,2,4; Lu, Junwan 3; Bao, Qiyu1,2,3,4; Zhang, Hailin1
发表日期	2022-11-01
发表期刊	FRONTIERS IN MICROBIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	AadA36 Providencia stuartii aminoglycoside nucleotidyltransferase novel aminoglycoside resistance gene kinetic analysis
其他关键词	CLASS 1 INTEGRON ; ANTIBIOTIC-RESISTANCE ; ANTIMICROBIAL RESISTANCE ; GENOME VISUALIZATION ; CLASS-1 INTEGRON ; GENE CASSETTE ; SEQUENCE ; PATHOGENICITY ; STREPTOMYCIN ; PROTEUS
摘要	In this study, we characterized a novel chromosome-encoded aminoglycoside nucleotidyltransferase (ANT), AadA36, from the Providencia stuartii strain P14 isolated from the sputum specimen of a burn patient at a hospital in Wenzhou, China. Among the functionally characterized ANTs, AadA36 shared the highest amino acid sequence identity of 51.91% with AadA14. The whole genome of P. stuartii P14 consisted of one chromosome and two plasmids (designated pP14-166 and pP14-114). A total of 19 genes with >= 80% similarity with functionally characterized antimicrobial resistance genes (ARGs) were identified in the whole genome, including aminoglycosides [aac(2 ')-Ia, aph(6)-Id, aph(3 '')-Ib, aac(6 ')-Ib, ant(3 '')-IIa, aph(3 ')-Ia], beta-lactams (bla(CMY-2) and bla(OXA-10)) and so on. Antimicrobial susceptibility testing showed that the aadA36 gene conferred specific resistance to spectinomycin and streptomycin, and the minimum inhibitory concentration (MIC) of these antimicrobials increased 128- and 64-fold compared with the control strain. The kinetic parameters of AadA36 were consistent with the MIC data of spectinomycin and streptomycin, with k(cat)/K-m ratios of (1.07 +/- 2.23) x 10(4) M-1 s(-1) and (8.96 +/- 1.01) x 10(3) M-1 s(-1), respectively. The identification of a novel aminoglycoside resistance gene will help us further understand the complexity of the resistance mechanisms and provide deep insights into the dissemination of resistance genes in the microbial population.
资助项目	Science & Technology Project of Wenzhou City[N20210001];Natural Science Foundation of China[81973382];Zhejiang Provincial Natural Science Foundation of China[LQ17H190001,LY19C060002];
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-302X
EISSN	1664-302X
卷号	13
DOI	10.3389/fmicb.2022.1035651
页数	11
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000885315200001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	36386671
SCOPUSEID	2-s2.0-85142040774
通讯作者地址	[Bao, Qiyu]Department of Children’s Respiration Disease,The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China ; [Zhang, Hailin]Department of Children’s Respiration Disease,The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Microbiology;Microbiology (medical)
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/171653
专题	附属第二医院检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Bao, Qiyu; Zhang, Hailin
作者单位	1.Department of Children’s Respiration Disease,The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China; 2.Key Laboratory of Medical Genetics of Zhejiang Province,Key Laboratory of Laboratory Medicine,Ministry of Education,China; 3.Medical Molecular Biology Laboratory,School of Medicine,Jinhua Polytechnic,Jinhua,China; 4.School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第二医院; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Gao, Mengdi,Feng, Chunlin,Ji, Yongan,et al. AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii[J]. FRONTIERS IN MICROBIOLOGY,2022,13.
APA	Gao, Mengdi., Feng, Chunlin., Ji, Yongan., Shi, Yaokai., Shi, Weina., ... & Zhang, Hailin. (2022). AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii. FRONTIERS IN MICROBIOLOGY, 13.
MLA	Gao, Mengdi,et al."AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of Providencia stuartii".FRONTIERS IN MICROBIOLOGY 13(2022).