A Novel and Reversible Experimental Primate Ocular Hypertension Model: Blocking Schlemm's Canal

doi:10.1159/000527099

科研成果详情

题名	A Novel and Reversible Experimental Primate Ocular Hypertension Model: Blocking Schlemm's Canal
作者	Jiang, Junhong 1; Xu, Jing1; Tao, Yan 1; Hu, Cheng 1; Zhang, Cong1; Sun, Xinmin 2; Ye, Cong1,3; Zhang, Shaodan1,3; Liang, Yuanbo1,3
发表日期	2022-11-15
发表期刊	OPHTHALMIC RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
其他关键词	ELEVATED INTRAOCULAR-PRESSURE ; EXPERIMENTAL GLAUCOMA ; TRABECULAR MESHWORK ; OUTFLOW RESISTANCE ; MOUSE MODEL ; RAT MODEL ; PROGRESSION ; CANALOPLASTY ; DAMAGE ; CELLS
摘要	Introduction: The purpose of this study was to establish a novel and reversible experimental ocular hypertension primate model by blocking Schlemm's canal. Methods: A model was induced in adult cynomolgus monkeys (n=4) by blocking Schlemm's canal with an inserted microcatheter (200 mu m diameter); it was removed 6 weeks later from one monkey to reverse the elevated intraocular hypertension. All animals were monitored for 11 months; weekly measurements of intraocular pressure and biweekly examinations with spectral domain optical coherence tomography and disc photography were performed. Histopathology of the eye and retinal ganglion cell counts were completed at the end of the study. Results: The intraocular pressure of the blocked eyes was significantly higher than that of the contralateral eyes at 1 month after the blockage (P <0.001); the mean intraocular pressure was similar to the contralateral eye from 1 week to 11 months after the microcatheter was removed in monkey A (P=0.170). The mean intraocular pressure of the blocked eyes of the remaining monkeys was significantly higher than that of the contralateral eyes throughout the follow-up period (P <0.001). The fundus imaging showed decreases in the retinal nerve fibre layer thickness, and localised defects were observed in two blocked eyes. A histological examination demonstrated that the number of retinal ganglion cells in blocked eyes of monkeys A, B, and C was significantly decreased compared with the control. Conclusion: Schlemm's canal blockage alone in the monkey model produces sustained elevation of intraocular pressure, which present a novel animal model for studying the pathogenesis of glaucoma.
资助项目	National Natural Science Foundation of China [81770919]; Natural Science Foundation of Zhejiang Province [LY17H120004]; Zhejiang Province Health innovation Talents Fund [2016025, LQ18H120010]; Zhejiang Provincial National science foundation of China [ZJCX-CZ-2016015]
出版者	KARGER
出版地	BASEL
ISSN	0030-3747
EISSN	1423-0259
卷号	66 期号:1 页码:354-366
DOI	10.1159/000527099
页数	20
WOS类目	Ophthalmology
WOS研究方向	Ophthalmology
WOS记录号	WOS:000888280900001
收录类别	SCIE ; SCOPUS
在线发表日期	2022-11
URL	查看原文
PubMed ID	36380650
SCOPUSEID	2-s2.0-85168676802
通讯作者地址	[Liang, Yuanbo]The Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Ophthalmology;Sensory Systems;Cellular and Molecular Neuroscience
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/171644
专题	眼视光学院（生物医学工程学院）、附属眼视光医院眼视光学院（生物医学工程学院）、附属眼视光医院_杭州院区_青光眼专科
通讯作者	Liang, Yuanbo
作者单位	1.The Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Wenzhou,China; 2.JOINN Laboratories,Suzhou,China; 3.Glaucoma Institute,Wenzhou Medical University,Wenzhou,China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Jiang, Junhong,Xu, Jing,Tao, Yan,et al. A Novel and Reversible Experimental Primate Ocular Hypertension Model: Blocking Schlemm's Canal[J]. OPHTHALMIC RESEARCH,2022,66(1):354-366.
APA	Jiang, Junhong., Xu, Jing., Tao, Yan., Hu, Cheng., Zhang, Cong., ... & Liang, Yuanbo. (2022). A Novel and Reversible Experimental Primate Ocular Hypertension Model: Blocking Schlemm's Canal. OPHTHALMIC RESEARCH, 66(1), 354-366.
MLA	Jiang, Junhong,et al."A Novel and Reversible Experimental Primate Ocular Hypertension Model: Blocking Schlemm's Canal".OPHTHALMIC RESEARCH 66.1(2022):354-366.