A newly isolated bacteriophage vB8388 and its synergistic effect with aminoglycosides against multi-drug resistant Klebsiella oxytoca strain FK-8388.

doi:10.1016/j.micpath.2022.105906

科研成果详情

题名	A newly isolated bacteriophage vB8388 and its synergistic effect with aminoglycosides against multi-drug resistant Klebsiella oxytoca strain FK-8388.
作者	Zhao, Yining1; Feng, Luozhu2; Zhou, Beibei1; Zhang, Xiaodong1; Yao, Zhuocheng2; Wang, Lingbo 1; Wang, Zhongyong1; Zhou, Tieli1; Chen, Lijiang1
发表日期	2023-01
发表期刊	Microbial pathogenesis 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Aminoglycoside Bacteriophage Klebsiella oxytoca Multidrug resistance Synergistic effect
其他关键词	INFECTION
摘要	The bacteriophage vB8388 can lyse multi-drug resistant Klebsiella oxytoca strain FK-8388 and maintain stability in a wide range of temperatures (from 4 °C to 80 °C) and pHs (3-11). Bioinformatics analysis showed that vB8388 is a linear double-stranded DNA virus that is 39,750 long with 50.65% G + C content and 44 putative open reading frames (ORFs). Phage vB8388 belongs to the family Autographviridae and possesses a non-contractile tail. The latency period of vB8388 was approximately 20 min. The combination of phage vB8388 and gentamicin, amikacin, or tobramycin could effectively inhibit the growth of K. oxytoca strain FK-8388, with a decrease of more than 4 log units within 12 h in vitro. Phage vB8388 showed a strong synergistic effect with gentamicin that could enhance the anti-biofilm effect of vB8388. The phage + gentamicin combination also showed synergy in vivo in the larval infection model of Galleria mellonella. In conclusion, the findings of this study suggest the potential of phage + antibiotic combination therapy to be used as an alternative therapeutic approach for treating infectious diseases caused by multidrug-resistant bacteria.
资助项目	National Natural Science Foundation of China; Major Projects of Wenzhou Science and Technology Bureau [82172328]; Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province [ZY2020006]; [2022E10022]
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
ISSN	0882-4010
EISSN	1096-1208
卷号	174 页码:105906
DOI	10.1016/j.micpath.2022.105906
页数	9
WOS类目	Immunology ; Microbiology
WOS研究方向	Immunology ; Microbiology
WOS记录号	WOS:000918240500004
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2022-12
URL	查看原文
PubMed ID	36494020
SCOPUSEID	2-s2.0-85143731225
通讯作者地址	[Zhou, Tieli]Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,Zhejiang Province,Wenzhou,China ; [Chen, Lijiang]Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,Zhejiang Province,Wenzhou,China
Scopus学科分类	Microbiology;Infectious Diseases
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/171544
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Zhou, Tieli; Chen, Lijiang
作者单位	1.Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province,Zhejiang Province,Wenzhou,China; 2.Department of Medical Lab Science,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Zhejiang Province,Wenzhou,China
第一作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 临床检验诊断学; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhao, Yining,Feng, Luozhu,Zhou, Beibei,et al. A newly isolated bacteriophage vB8388 and its synergistic effect with aminoglycosides against multi-drug resistant Klebsiella oxytoca strain FK-8388.[J]. Microbial pathogenesis,2023,174:105906.
APA	Zhao, Yining., Feng, Luozhu., Zhou, Beibei., Zhang, Xiaodong., Yao, Zhuocheng., ... & Chen, Lijiang. (2023). A newly isolated bacteriophage vB8388 and its synergistic effect with aminoglycosides against multi-drug resistant Klebsiella oxytoca strain FK-8388.. Microbial pathogenesis, 174, 105906.
MLA	Zhao, Yining,et al."A newly isolated bacteriophage vB8388 and its synergistic effect with aminoglycosides against multi-drug resistant Klebsiella oxytoca strain FK-8388.".Microbial pathogenesis 174(2023):105906.