bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1-JNK Pathways

doi:10.7150/ijbs.11921

科研成果详情

题名	bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1-JNK Pathways
作者	Shi, Hongxue1; Cheng, Yi 1; Ye, Jingjing1; Cai, Pingtao 1; Zhang, Jinjing 1; Li, Rui 1; Yang, Ying 1; Wang, Zhouguang 1; Zhang, Hongyu1; Lin, Cai2; Lu, Xianghong 3; Jiang, Liping1; Hu, Aiping1; Zhu, Xinbo1; Zeng, Qiqiang 2; Fu, Xiaobing 4; Li, Xiaokun1; Xiao, Jian1
发表日期	2015-12-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	bFGF human dermal fibroblast
其他关键词	GROWTH-FACTOR ; CELL-MIGRATION ; ADVANCED GLYCATION ; OXIDATIVE STRESS ; NADPH OXIDASE ; HIGH-GLUCOSE ; SIGNALING PATHWAY ; BINDING-PROTEIN ; RAT MODEL ; ROS
摘要	Fibroblasts play a pivotal role in the process of cutaneous wound repair, whereas their migratory ability under diabetic conditions is markedly reduced. In this study, we investigated the effect of basic fibroblast growth factor (bFGF) on human dermal fibroblast migration in a high-glucose environment. bFGF significantly increased dermal fibroblast migration by increasing the percentage of fibroblasts with a high polarity index and reorganizing F-actin. A significant increase in intracellular reactive oxygen species (ROS) was observed in dermal fibroblasts under diabetic conditions following bFGF treatment. The blockage of bFGF-induced ROS production by either the ROS scavenger N-acetyl-L-cysteine (NAC) or the NADPH oxidase inhibitor diphenylene iodonium chloride (DPI) almost completely neutralized the increased migration rate of dermal fibroblasts promoted by bFGF. Akt, Rac1 and JNK were rapidly activated by bFGF in dermal fibroblasts, and bFGF-induced ROS production and promoted dermal fibroblast migration were significantly attenuated when suppressed respectively. In addition, bFGF-induced increase in ROS production was indispensable for the activation of focal adhesion kinase (FAK) and paxillin. Therefore, our data suggested that bFGF promotes the migration of human dermal fibroblasts under diabetic conditions through increased ROS production via the PI3K/Akt-Rac1-JNK pathways.
资助项目	Zhejiang Provincial Program for the Cultivation of High-level Innovative Health talents; National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112, 81372064, 81302775, 81472165]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y14H150023, Y14H090062, LY13H030008, LY14H150008]; State Key Basic Research Development Program [2012CB518105]; Zhejiang Provincial Project of Key Group [2010R50042]
出版者	IVYSPRING INT PUBL
出版地	LAKE HAVEN
ISSN	1449-2288
卷号	11 期号:7 页码:845-859
DOI	10.7150/ijbs.11921
页数	15
WOS类目	Biochemistry & Molecular Biology ; Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000359007700012
收录类别	SCIE ; SCOPUS
URL	查看原文
PubMed ID	26078726
SCOPUSEID	2-s2.0-84933497356
通讯作者地址	[Xiao, Jian]School of Pharmaceutical Sciences,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Ecology, Evolution, Behavior and Systematics;Applied Microbiology and Biotechnology;Molecular Biology;Developmental Biology;Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17091
专题	药学院（分析测试中心）_生物制药系_生物制药工程附属第一医院研究生工作部（研究生院）其他_附属第六医院（丽水市人民医院）
通讯作者	Xiao, Jian
作者单位	1.School of Pharmaceutical Sciences,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou,325035,China; 2.The First Affiliate Hospital,Wenzhou Medical University,Wenzhou,325035,China; 3.Translation Medicine Research Center,Lishui People’s Hospital,Wenzhou Medical University,Lishui,323000,China; 4.Wound Healing and Cell Biology Laboratory,Institute of Basic Medical Science,Chinese PLA General Hospital,Beijing,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Shi, Hongxue,Cheng, Yi,Ye, Jingjing,et al. bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1-JNK Pathways[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2015,11(7):845-859.
APA	Shi, Hongxue., Cheng, Yi., Ye, Jingjing., Cai, Pingtao., Zhang, Jinjing., ... & Xiao, Jian. (2015). bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1-JNK Pathways. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 11(7), 845-859.
MLA	Shi, Hongxue,et al."bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1-JNK Pathways".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 11.7(2015):845-859.