The Possible Effect of B-Cell Epitopes of Epstein-Barr Virus Early Antigen, Membrane Antigen, Latent Membrane Protein-1, and-2A on Systemic Lupus Erythematosus

doi:10.3389/fimmu.2018.00187

科研成果详情

题名	The Possible Effect of B-Cell Epitopes of Epstein-Barr Virus Early Antigen, Membrane Antigen, Latent Membrane Protein-1, and-2A on Systemic Lupus Erythematosus
作者	Tu, Jianxin1; Wang, Xiaobing1; Geng, Guannan 2; Xue, Xiangyang3; Lin, Xiangyang4; Zhu, Xiaochun1; Sun, Li1
发表日期	2018-02-12
发表期刊	FRONTIERS IN IMMUNOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Epstein-Barr virus systemic lupus erythematosus B-cell epitopes cross-reactivity early antigen protein D envelope glycoprotein GP340/membrane antigen latent membrane protein-1 latent membrane protein-2A
其他关键词	KEYHOLE LIMPET HEMOCYANIN ; MOLECULAR MIMICRY ; INFECTIOUS-MONONUCLEOSIS ; AUTOIMMUNE-DISEASE ; NUCLEAR ANTIGEN-1 ; 2 LMP2 ; EBV ; INTERFERON ; EXPRESSION ; ANTIBODIES
摘要	This study was aimed to evaluate the role of B-cell epitopes of Epstein-Barr virus (EBV) Early antigen protein D (EA), envelope glycoprotein GP340/membrane antigen (MA), latent membrane protein (LMP)-1, and LMP-2A in systemic lupus erythematosus (SLE). B-cell epitopes were predicted by analyzing secondary structure, transmembrane domains, surface properties, and homological comparison. 60 female mice were randomized equally into 12 groups: 1-10 groups were immunized by epitope peptides (EPs) 1-10, respectively, while 11 and 12 groups were PBS and Keyhole limpet hemocyanin (KLH) control groups. Immunoglobulin G (IgG) and autoantibody to nuclear antigen (ANA) concentrations in mice serum were determined at week 8. Indirect levels of EP1-10 were further detected by enzyme-linked immuno sorbent assay (ELISA) in 119 SLE patients and 64 age-and gender-matched health controls (HCs). 10 probable EBV EA, MA, LMP-1, and LMP-2A B-cell epitopes related to SLE self-antigens were predicted and corresponding EP1-10 were synthesized. IgG concentrations at week 8 were increased in EP1-10 and KLH groups compared with PBS group in mice; while ANA levels were elevated in only EP1-4, EP6-7, and EP10 groups compared to KLH group by ELISA, and ANA-positive rates were increased in only EP1, EP2, EP4, EP6, and EP10 groups by indirect immunofluorescence assay. EP1-4, EP6, and EP10 indirect levels were increased in SLE patients than HCs, while EP1, EP3, EP6, and EP9 were correlated with SLE disease activity index score. In conclusion, EBV EA, MA, LMP-1, and LMP-2A B-cell EPs increased SLE-related autoantibodies in mice, and their indirect levels might be served as potential biomarkers for SLE diagnosis and disease severity.
资助项目	Medical Scientific Research Foundation of Zhejiang Province, China [2012KYA130]; Science and Technology Planning Project of Wenzhou [Y20170056]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-3224
卷号	9 期号:FEB 页码:187
DOI	10.3389/fimmu.2018.00187
页数	13
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:000424779800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29497417
PMC记录号	PMC5819577
SCOPUSEID	2-s2.0-85041900435
通讯作者地址	[Sun, Li]Department of Rheumatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17049
专题	附属第一医院基础医学院（机能实验教学中心）基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Sun, Li
作者单位	1.Department of Rheumatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Central Laboratory,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China; 3.Department of Microbiology and Immunology,Basic Medical College of Wenzhou Medical University,Wenzhou,China; 4.Medical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Tu, Jianxin,Wang, Xiaobing,Geng, Guannan,et al. The Possible Effect of B-Cell Epitopes of Epstein-Barr Virus Early Antigen, Membrane Antigen, Latent Membrane Protein-1, and-2A on Systemic Lupus Erythematosus[J]. FRONTIERS IN IMMUNOLOGY,2018,9(FEB):187.
APA	Tu, Jianxin., Wang, Xiaobing., Geng, Guannan., Xue, Xiangyang., Lin, Xiangyang., ... & Sun, Li. (2018). The Possible Effect of B-Cell Epitopes of Epstein-Barr Virus Early Antigen, Membrane Antigen, Latent Membrane Protein-1, and-2A on Systemic Lupus Erythematosus. FRONTIERS IN IMMUNOLOGY, 9(FEB), 187.
MLA	Tu, Jianxin,et al."The Possible Effect of B-Cell Epitopes of Epstein-Barr Virus Early Antigen, Membrane Antigen, Latent Membrane Protein-1, and-2A on Systemic Lupus Erythematosus".FRONTIERS IN IMMUNOLOGY 9.FEB(2018):187.