MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A

doi:10.3892/or.2014.3303

科研成果详情

题名	MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A
作者	Ye, Wenwei 1,3; Xue, Jisen1; Zhang, Qian 1; Li, Fuyao1; Zhang, Wei 2; Chen, Huijun 1; Huang, Yibo 1; Zheng, Feiyun 1
发表日期	2014-09
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	microRNA-449a endometrial cancer proliferation apoptosis invasion CDC25A
其他关键词	HEPATOCELLULAR-CARCINOMA CELLS ; GROWTH ; PROLIFERATION ; MICRORNAS
摘要	Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development by functioning either as oncogenes or as tumor suppressors. The role of microRNA-449a (miR-449a) in endometrial cancer remains unclear. We examined the levels of miR-449a and miR-449b in benign endometrium, type I and type II endometrial cancer tissues by quantitative real-time polymerase chain reaction. To further investigate the roles of miR-449a in regulating the behavior of endometrial cancer cells, we overexpressed miR-449a in the endometrial cancer cell line HEC-1B, which had low endogenous miR-449a expression. We analyzed the effects of miR-449a overexpression on CDC25 expression, proliferation, invasion and apoptosis of HEC-1B cells. We found that miR-449a and miR-449b levels were markedly reduced in type II endometrial cancer tissues but not in type I endometrial cancer tissues compared with normal endometrium. Overexpression of miR-449a significantly inhibited the proliferation, invasion and clonogenic survival of HEC-1B cells. MiR-449a overexpression also induced apoptosis in HEC-1B cells. In addition, real-time RT-PCR and western blot analysis showed that CDC25A expression was suppressed by miR-449a overexpression. Our results suggest that miR-449a may act as a tumor suppressor by targeting CDC25A in endometrial cancer.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2090699]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	32 期号:3 页码:1193-1199
DOI	10.3892/or.2014.3303
页数	7
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000339983200042
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	24993091
SCOPUSEID	2-s2.0-84923068196
通讯作者地址	[Zheng, Feiyun]Department of Gynecology, Wenzhou Medical University,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16966
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_外科学_肿瘤外科
通讯作者	Zheng, Feiyun
作者单位	1.Department of Gynecology, Wenzhou Medical University,China; 2.Department of Surgical Oncology, Wenzhou Medical University,China; 3.Department of Gynecology, Taizhou Municipal Hospital,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Ye, Wenwei,Xue, Jisen,Zhang, Qian,et al. MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A[J]. ONCOLOGY REPORTS,2014,32(3):1193-1199.
APA	Ye, Wenwei., Xue, Jisen., Zhang, Qian., Li, Fuyao., Zhang, Wei., ... & Zheng, Feiyun. (2014). MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A. ONCOLOGY REPORTS, 32(3), 1193-1199.
MLA	Ye, Wenwei,et al."MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A".ONCOLOGY REPORTS 32.3(2014):1193-1199.