通过ERK信号通路逆转IL-1β介导的人胚肺成纤维细胞转分化(英文)

科研成果详情

题名	通过ERK信号通路逆转IL-1β介导的人胚肺成纤维细胞转分化(英文)
作者	Long-teng Jin ; He Zhang ; Chang-chong Li ; Li Lin ; Wei-xi Zhang
会议录名称	2014年浙江省医学会儿科学分会学术年会暨儿内科疾病诊治新进展国家级继续教育学习班论文汇编影响因子和分区
语种	英语
原始文献类型	中国会议
摘要	Objective:The purpose of the present study was to determine whether Interleukin(IL)-1β-mediated human embryonic pulmonary fibroblast transdifferentiation could be reversed by targeting of the ERK signaling pathway.Methods:The human embryonic pulmonary fibroblast MRC-5 cell line was used as a model to observe IL-1β-mediated transdifferentiation as well as the inhibitory effects of lentinan(LNT).Cell proliferation was examined using a CCK-8 assay.ERK signaling activity was detected using immonublotting with phospho-ERK antibody.Expression levels of fibronectin(FN),Col Ⅰ and α-smooth muscle actin(α-SMA)were assessed by either reverse transcription-PCR or the SABC assay.Results:IL-1β-induced ERK signaling activation in MRC-5 cells could be inhibited by pretreatment with the LNT or ERK inhibitor U0126.Meanwhile,IL-1β-enhanced cell proliferation and expression of FN,Col Ⅰ and a-SMA were also attenuated by treatment with LNT.Conclusion:Our study revealed that activation of ERK signaling is involved in IL-1β-mediated human embryonic pulmonary fibroblast proliferation,phenotypic switching and collagen secretion.However,these transdifferentiation events in MRC-5 cells could be reversed with LNT treatment by targeting of the ERK signaling pathway.
页码	1
收录类别	CNKI
发表日期	2014-05-15
文献类型	会议论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167922
专题	第二临床医学院，附属第二医院、育英儿童医院
作者单位	1.Department of Pediatric infectious disease 2.The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
推荐引用方式 GB/T 7714	Long-teng Jin,He Zhang,Chang-chong Li,等. 通过ERK信号通路逆转IL-1β介导的人胚肺成纤维细胞转分化(英文)[C],2014:1.