Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury

doi:10.4103/1673-5374.295347

科研成果详情

题名	Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury
作者	Ye, Lu-Xia 1; An, Ning-Chen 1; Huang, Peng2; Li, Duo-Hui 1; Zheng, Zhi-Long 1; Ji, Hao 3; Li, Hao 4; Chen, Da-Qing 4; Wu, Yan-Qing 3; Xiao, Jian1; Xu, Ke 3; Li, Xiao-Kun1; Zhang, Hong-Yu1
发表日期	2021-04
发表期刊	NEURAL REGENERATION RESEARCH 影响因子和分区
语种	英语
原始文献类型	Article
关键词	autophagy blood-spinal cord barrier central nervous system locomotor function neurovascular unit platelet-derived growth factor spinal cord spinal cord injury
其他关键词	ENDOPLASMIC-RETICULUM STRESS ; FUNCTIONAL RECOVERY ; PDGF-BB ; BARRIER DISRUPTION ; LOCOMOTOR RECOVERY ; SECONDARY INJURY ; AUTOPHAGY ; INTEGRITY ; DELIVERY ; DISEASE
摘要	The blood-spinal cord barrier plays a vital role in recovery after spinal cord injury. The neurovascular unit concept emphasizes the relationship between nerves and vessels in the brain, while the effect of the blood-spinal cord barrier on the neurovascular unit is rarely reported in spinal cord injury studies. Mouse models of spinal cord injury were established by heavy object impact and then immediately injected with platelet-derived growth factor (80 mu g/kg) at the injury site. Our results showed that after platelet-derived growth factor administration, spinal cord injury, neuronal apoptosis, and blood-spinal cord barrier permeability were reduced, excessive astrocyte proliferation and the autophagy-related apoptosis signaling pathway were inhibited, collagen synthesis was increased, and mouse locomotor function was improved. In vitro, human umbilical vein endothelial cells were established by exposure to 200 mu M H2O2. At 2 hours prior to injury, in vitro cell models were treated with 5 ng/mL platelet-derived growth factor. Our results showed that expression of blood-spinal cord barrier-related proteins, including Occludin, Claudin 5, and beta-catenin, was significantly decreased and autophagy was significantly reduced. Additionally, the protective effects of platelet-derived growth factor could be reversed by intraperitoneal injection of 80 mg/kg chloroquine, an autophagy inhibitor, for 3 successive days prior to spinal cord injury. Our findings suggest that platelet-derived growth factor can promote endothelial cell repair by regulating autophagy, improve the function of the blood-spinal cord barrier, and promote the recovery of locomotor function post-spinal cord injury. Approval for animal experiments was obtained from the Animal Ethics Committee, Wenzhou Medical University, China (approval No. wydw2018-0043) in July 2018.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81802251, 81772450, 81801233]; Natural Science Foundation of Zhejiang Province of ChinaNatural Science Foundation of Zhejiang Province [LQ18H150003, LY19H150001, LQ18H090011, LQ20C200015]; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [YKFJ3-011]
出版者	WOLTERS KLUWER MEDKNOW PUBLICATIONS
出版地	MUMBAI
ISSN	1673-5374
EISSN	1876-7958
卷号	16 期号:4 页码:765-771
DOI	10.4103/1673-5374.295347
页数	7
WOS类目	Cell Biology ; Neurosciences
WOS研究方向	Cell Biology ; Neurosciences & Neurology
WOS记录号	WOS:000585772900033
收录类别	SCIE ; PUBMED ; SCOPUS ; CSCD
学科领域	医药、卫生 ; 基础医学 ; 临床医学 ; 神经病学与精神病学
URL	查看原文
CSCD记录号	CSCD:6902973
PubMed ID	33063740
PMC记录号	PMC8067950
SCOPUSEID	2-s2.0-85094600726
通讯作者地址	[Li, Xiao-Kun]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,China ; [Zhang, Hong-Yu]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,China ; [Xu, Ke]Institute of Life Sciences,Wenzhou University,Wenzhou, Zhejiang Province,China
Scopus学科分类	Developmental Neuroscience
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16756
专题	药学院（分析测试中心）附属第二医院第二临床医学院、附属第二医院、育英儿童医院研究生工作部（研究生院）其他_附属第三医院（瑞安市人民医院）其他_附属第六医院（丽水市人民医院）
通讯作者	Xu, Ke; Li, Xiao-Kun; Zhang, Hong-Yu
作者单位	1.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou, Zhejiang Province,China; 2.Department of Pharmacy,Ruian People's Hospital,Third Affiliated Hospital of Wenzhou Medical University,Wenzhou, Zhejiang Province,China; 3.Institute of Life Sciences,Wenzhou University,Wenzhou, Zhejiang Province,China; 4.Department of Orthopedics Surgery,Lishui People's Hospital,Sixth Affiliated Hospital of Wenzhou Medical University,Lishui, Zhejiang Province,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Ye, Lu-Xia,An, Ning-Chen,Huang, Peng,et al. Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury[J]. NEURAL REGENERATION RESEARCH,2021,16(4):765-771.
APA	Ye, Lu-Xia., An, Ning-Chen., Huang, Peng., Li, Duo-Hui., Zheng, Zhi-Long., ... & Zhang, Hong-Yu. (2021). Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury. NEURAL REGENERATION RESEARCH, 16(4), 765-771.
MLA	Ye, Lu-Xia,et al."Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury".NEURAL REGENERATION RESEARCH 16.4(2021):765-771.

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