A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients

doi:10.1177/030089160909500311

科研成果详情

题名	A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients
作者	Wu, Cun-Zao 1; Ni, Xiao-Jie1; Zheng, Shao-Ling1; Yang, Yi-Rong1; Xia, Peng1; Zeng, Yan-Jun 2; Chen, Bi-Cheng 1
发表日期	2009-05
发表期刊	TUMORI JOURNAL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	ATP-binding cassette subfamily B member 1 (ABCB1) single nucleotide polymorphisms (SNPs) sequence-specific primer polymerase chain reaction (SSP-PCR)
其他关键词	MULTIDRUG-RESISTANCE GENE ; SINGLE-NUCLEOTIDE POLYMORPHISMS ; POLYMERASE-CHAIN-REACTION ; HUMAN MDR1 GENE ; P-GLYCOPROTEIN ; SEQUENCE VARIATIONS ; SUBSTRATE ; EXPRESSION ; DIGOXIN ; ALLELE
摘要	Aim. P-glycoprotein, the product of the ATP-binding cassette subfamily B member 1. (ABCB1) gene (or the so-called multidrug resistance I gene), is an ATP-driven efflux pump contributing to the pharmacokinetics as well as the pharmacokinetics of drugs that are P-glycoprotein substrates, such as tacrolimus. This paper describes the development of a new method for detection of the 3435C/T and 2677G/T/A single nucleotide polymorphisms of the ABCB1 gene. The method is a simple sequence-specific primer polymerase chain reaction (SSP-PCR). Methods. 158 Chinese health checkup examinees and 214 transplant recipients were included in the study. Genomic DNA was extracted from peripheral blood and amplified with SSP-PCR to detect the 3435C/T and 2677G/T/A mutations in ABCB1. The SSP-PCR condition was optimized, and the PCR results were compared with those of DNA sequencing. Results. In the optimized condition, the two polymorphisms could be clearly distinguished after one-step PCR and electrophoresis. The ABCB1 3435C/T and 2677G/T/A genotypes of the subjects were scanned, and allele-specific bands were successfully amplified by SSP-PCR, which were in full accordance with the results of sequencing. Conclusion. As a fast, simple and inexpensive genotyping tool, the method would be practicable in large clinical studies on interindividual pharmacokinetics.
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	0300-8916
EISSN	2038-2529
卷号	95 期号:3 页码:338-342
DOI	10.1177/030089160909500311
页数	5
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000268054000011
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	19688973
SCOPUSEID	2-s2.0-67849109681
通讯作者地址	[Zeng, Yan-Jun]Biomechanics and Medical Information Institute,Beijing University of Technology,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16726
专题	第一临床医学院（信息与工程学院）、附属第一医院_外科学_泌尿外科附属第一医院
通讯作者	Zeng, Yan-Jun
作者单位	1.Transplantation Center,Wenzhou Medical College,China; 2.Biomechanics and Medical Information Institute,Beijing University of Technology,China
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Wu, Cun-Zao,Ni, Xiao-Jie,Zheng, Shao-Ling,et al. A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients[J]. TUMORI JOURNAL,2009,95(3):338-342.
APA	Wu, Cun-Zao., Ni, Xiao-Jie., Zheng, Shao-Ling., Yang, Yi-Rong., Xia, Peng., ... & Chen, Bi-Cheng. (2009). A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients. TUMORI JOURNAL, 95(3), 338-342.
MLA	Wu, Cun-Zao,et al."A fast SSP-PCR method for genotyping the ATP-binding cassette subfamily B member 1 gene C3435T and G2677T polymorphisms in Chinese transplant recipients".TUMORI JOURNAL 95.3(2009):338-342.