A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits

doi:10.14336/AD.2022.0722

科研成果详情

题名	A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits
作者	Lin, Xiao1,2; Wang, Hua1,2; Huang, Shengwei 1,2; Chen, Lefu1,2; Yang, Su 2; Zhao, Peiqi 1,2; Lin, Zhongxiao 1,2; Yang, Jianjing1,2; Ruan, Linhui1,2; Ni, Haoqi1,2; Wang, Kankai1,2; Wen, Min 3; Jin, Kunlin 4; Zhuge, Qichuan1,2
发表日期	2023-02
发表期刊	AGING AND DISEASE 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	ferric chloride cynomolgus macaque nonhuman primate ischemic stroke middle cerebral artery
其他关键词	MIDDLE CEREBRAL-ARTERY ; PLASMINOGEN-ACTIVATOR INHIBITOR-1 ; BRAIN-DAMAGE ; OCCLUSION ; THROMBOLYSIS ; THROMBOSIS ; PRASUGREL ; MONKEYS ; INJURY ; REPAIR
摘要	A nonhuman primate model of ischemic stroke is considered as an ideal preclinical model to replicate various aspects of human stroke because of their similarity to humans in genetics, neuroanatomy, physiology, and immunology. However, it remains challenging to produce a reliable and reproducible stroke model in nonhuman primates due to high mortality and variable outcomes. Here, we developed a focal cerebral ischemic model induced by topical application of 50% ferric chloride (FeCl3) onto the MCA-M1 segment through a cranial window in the cynomolgus monkeys. We found that FeCl3 rapidly produced a stable intraarterial thrombus that caused complete occlusion of the MCA, leading to the quick decrease of the regional cerebral blood flow in 10 min. A typical cortical infarct was detected 24 hours by magnetic resonance imaging (MRI) and was stable at least for 1 month after surgery. The sensorimotor deficit assessed by nonhuman primate stroke scale was observed at 1 day and up to 3 months after ischemic stroke. No spontaneous revascularization or autolysis of thrombus was observed, and vital signs were not affected. All operated cynomolgus monkeys survived. Our data suggested that FeCl3-induced stroke in nonhuman primates was a replicable and reliable model that is necessary for the correct prediction of the relevance of experimental therapeutic approaches in human beings.
资助项目	National Natural Science Foundation of China; Zhejiang provincial natural Science Foundation; Major Science and Technology Project of Wenzhou Science and Technology Bureau; [81820108011]; [82001225]; [2020C03022]; [2017C03027]; [ZS2017007]
出版者	INT SOC AGING & DISEASE
出版地	FORT WORTH
ISSN	2152-5250
卷号	14 期号:1 页码:245-255
DOI	10.14336/AD.2022.0722
页数	11
WOS类目	Geriatrics & Gerontology
WOS研究方向	Geriatrics & Gerontology
WOS记录号	WOS:000868287700001
收录类别	SCIE ; SCOPUS ; PUBMED
在线发表日期	2022-08
URL	查看原文
PubMed ID	36818571
SCOPUSEID	2-s2.0-85161688785
通讯作者地址	[Zhuge, Qichuan]First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Pathology and Forensic Medicine;Geriatrics and Gerontology;Neurology (clinical);Cell Biology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167247
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_老化与神经疾病重点实验室
通讯作者	Zhuge, Qichuan
作者单位	1.Department of Neurosurgery,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,Wenzhou Medical University,Wenzhou,China; 3.Department of Neurology,Guangzhou First People's Hospital,Guangzhou,China; 4.Department of Pharmacology and Neuroscience,University of North Texas Health Science Center,Fort Worth,76107,United States
第一作者单位	附属第一医院; 温州医科大学
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Lin, Xiao,Wang, Hua,Huang, Shengwei,et al. A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits[J]. AGING AND DISEASE,2023,14(1):245-255.
APA	Lin, Xiao., Wang, Hua., Huang, Shengwei., Chen, Lefu., Yang, Su., ... & Zhuge, Qichuan. (2023). A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits. AGING AND DISEASE, 14(1), 245-255.
MLA	Lin, Xiao,et al."A Reliable Nonhuman Primate Model of Ischemic Stroke with Reproducible Infarct Size and Long-term Sensorimotor Deficits".AGING AND DISEASE 14.1(2023):245-255.