The dual role of interleukin-17 in the growth of human papillomavirus-negative cervical cancer cells

doi:10.1177/1721727X221128089

科研成果详情

题名	The dual role of interleukin-17 in the growth of human papillomavirus-negative cervical cancer cells
作者	Pan, Yifan 1; Luo, Xishao 2; Ma, Jinghang2; Feng, Zhen3; Hu, Yan2; Xue, Jisen2
发表日期	2022-09
发表期刊	EUROPEAN JOURNAL OF INFLAMMATION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	interleukin-17 cervical cancer apoptosis vascular endothelial growth factor
其他关键词	IL-17 FAMILY ; CYTOKINES
摘要	To explore the relationship between IL-17 mediated immune response and HPV-negative cervical cancer, we established the co-culture system of cervical cancer C-33A cells and PBMC, and treated with different concentrations of rhIL-17. PBMC were extracted from 10ml anticoagulant blood provided by healthy female volunteers and colleagues in our hospital, which were co-cultured with cervical cancer C-33A cells. After treatment with different concentrations of rhIL-17, the proliferation, apoptosis, invasion behavior and VEGF expression level of cancer cells in each group, were determined by CCK-8, flow cytometry, transwell invasion assay and ELISA, respectively. The proliferation rate of C-33A cells slightly increased with the ascending of IL-17 concentration, but no statistical significance was found among each group (P > 0.05). When at low concentration of IL-17, the cell apoptosis rate seems to slightly decline. With the increase of concentration, the decrease of apoptosis rate became more obvious (P < 0.05). However, differing from those shown above, we found that IL-17 slightly inhibited the cancer cell invasion at high concentration, while it was more obvious at low concentration (P < 0.05). Furthermore, we found that there was no significant correlation between IL-17 and VEGF (P > 0.05). In summary, there is a close correlation between IL-17 mediated immune response and HPV-negative cervical cancer. Also, we think that IL-17 may play a dual role in tumor progression and could be a possible significant influence on tumor proliferation, apoptosis, and metastasis, which provides a basic theoretical basis for the further study of immunotherapy measures for cervical cancer.
资助项目	Wenzhou Municipal Science and Technology Bureau [Y20170119]
出版者	SAGE PUBLICATIONS INC
出版地	THOUSAND OAKS
ISSN	1721-727X
EISSN	2058-7392
卷号	20
DOI	10.1177/1721727X221128089
页数	8
WOS类目	Immunology
WOS研究方向	Immunology
WOS记录号	WOS:000868695300001
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85139677956
通讯作者地址	[Hu, Yan]Department of Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China ; [Xue, Jisen]Department of Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Immunology and Allergy;Immunology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167205
专题	附属第一医院_麻醉科第一临床医学院（信息与工程学院）、附属第一医院_妇产科学_妇科
通讯作者	Hu, Yan; Xue, Jisen
作者单位	1.Department of Anesthesiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Gynecology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 3.Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Pan, Yifan,Luo, Xishao,Ma, Jinghang,et al. The dual role of interleukin-17 in the growth of human papillomavirus-negative cervical cancer cells[J]. EUROPEAN JOURNAL OF INFLAMMATION,2022,20.
APA	Pan, Yifan, Luo, Xishao, Ma, Jinghang, Feng, Zhen, Hu, Yan, & Xue, Jisen. (2022). The dual role of interleukin-17 in the growth of human papillomavirus-negative cervical cancer cells. EUROPEAN JOURNAL OF INFLAMMATION, 20.
MLA	Pan, Yifan,et al."The dual role of interleukin-17 in the growth of human papillomavirus-negative cervical cancer cells".EUROPEAN JOURNAL OF INFLAMMATION 20(2022).