Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo.

doi:10.1016/j.hbpd.2022.10.007

科研成果详情

题名	Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo.
作者	Zhang, Jian-Se 1,2,3; Wang, Zhi-Bin1,2,3; Lai, Zhi-Zhen 4; Yang, Jing-Wen5; Song, Wen-Jing 6; Wei, Yu-Bing1; Mei, Jin 2,7; Wang, Jian-Guang8
发表日期	2022-10-23
发表期刊	Hepatobiliary & pancreatic diseases international : HBPD INT 影响因子和分区
语种	英语
原始文献类型	Journal Article ; Article
关键词	Angiogenesis Decellularization Liver tissue engineering Polyethylene glycol Single liver lobe
其他关键词	EXTRACELLULAR-MATRIX ; IMMUNOGENICITY ; DELIVERY ; PEG
摘要	Improving the mechanical properties and angiogenesis of acellular scaffolds before transplantation is an important challenge facing the development of acellular liver grafts. The present study aimed to evaluate the cytotoxicity and angiogenesis of polyethylene glycol (PEG) crosslinked decellularized single liver lobe scaffolds (DLSs), and establish its suitability as a graft for long-term liver tissue engineering., Using mercaptoacrylate produced by the Michael addition reaction, DLSs were first modified using N-succinimidyl S-acetylthioacetate (SATA), followed by cross-linking with PEG as well as vascular endothelial growth factor (VEGF). The optimal concentration of agents and time of the individual steps were identified in this procedure through biomechanical testing and morphological analysis. Subsequently, human umbilical vein endothelial cells (HUVECs) were seeded on the PEG crosslinked scaffolds to detect the proliferation and viability of cells. The scaffolds were then transplanted into the subcutaneous tissue of Sprague-Dawley rats to evaluate angiogenesis. In addition, the average number of blood vessels was evaluated in the grafts with or without PEG at days 7, 14, and 21 after implantation., The PEG crosslinked DLS maintained their three-dimensional structure and were more translucent after decellularization than native DLS, which presented a denser and more porous network structure. The results for Young's modulus proved that the mechanical properties of 0.5 PEG crosslinked DLS were the best and close to that of native livers. The PEG-VEGF-DLS could better promote cell proliferation and differentiation of HUVECs compared with the groups without PEG cross-linking. Importantly, the average density of blood vessels was higher in the PEG-VEGF-DLS than that in other groups at days 7, 14, and 21 after implantation in vivo., The PEG crosslinked DLS with VEGF could improve the biomechanical properties of native DLS, and most importantly, their lack of cytotoxicity provides a new route to promote the proliferation of cells in vitro and angiogenesis in vivo in liver tissue engineering.
资助项目	Wenzhou Medical University[2021-0469];National Natural Science Foundation of China[81970653];Natural Science Foundation of Zhejiang Province[LY20H180011];Medical and Health Science and Technology project of Zhejiang[2016KYA061];
出版者	ELSEVIER
ISSN	1499-3872
EISSN	2352-9377
卷号	22 期号:6 页码:622-631
DOI	10.1016/j.hbpd.2022.10.007
页数	10
WOS类目	Gastroenterology & Hepatology
WOS研究方向	Gastroenterology & Hepatology
WOS记录号	WOS:001110196200001
收录类别	PUBMED ; SCOPUS ; SCIE
在线发表日期	2023-11
URL	查看原文
PubMed ID	36335030
SCOPUSEID	2-s2.0-85143511037
通讯作者地址	[Wang, Jian-Guang]Department of Biochemistry,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Hepatology;Gastroenterology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167119
专题	基础医学院（机能实验教学中心）附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_生物化学与分子生物学系检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Wang, Jian-Guang
作者单位	1.Anatomy Department,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325000,China; 2.Institute of Bioscaffold Transplantation and Immunology,Wenzhou Medical University,Wenzhou,325000,China; 3.Institute of Hypoxic Medicine,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325000,China; 4.Intensive Care Unit,Tongde Hospital of Zhejiang Province,Hangzhou,310012,China; 5.Department of Geriatric Medicine,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 6.Department of Microbiology and Immunology,Wenzhou Medical University,Wenzhou,325000,China; 7.Medical Research Center,Ningbo City First Hospital,Ningbo,315000,China; 8.Department of Biochemistry,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	基础医学院（机能实验教学中心）; 温州医科大学
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Zhang, Jian-Se,Wang, Zhi-Bin,Lai, Zhi-Zhen,et al. Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo.[J]. Hepatobiliary & pancreatic diseases international : HBPD INT,2022,22(6):622-631.
APA	Zhang, Jian-Se., Wang, Zhi-Bin., Lai, Zhi-Zhen., Yang, Jing-Wen., Song, Wen-Jing., ... & Wang, Jian-Guang. (2022). Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo.. Hepatobiliary & pancreatic diseases international : HBPD INT, 22(6), 622-631.
MLA	Zhang, Jian-Se,et al."Polyethylene glycol crosslinked decellularized single liver lobe scaffolds with vascular endothelial growth factor promotes angiogenesis in vivo.".Hepatobiliary & pancreatic diseases international : HBPD INT 22.6(2022):622-631.