Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq.

doi:10.3389/fgene.2022.1023041

科研成果详情

题名	Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq.
作者	Yang, Xu1; Ji, Jingzhang1; Cui, Hongdi 1; Zhao, Qi 2; Ding, Chunming1; Xu, Chang 3
发表日期	2022-10-13
发表期刊	Frontiers in genetics 影响因子和分区
语种	英语
原始文献类型	Article
关键词	LTR RNA-seq embryo lncRNA pig small RNA-seq
其他关键词	CAP ANALYSIS ; GENE ; EXPRESSION ; ACTIVATION ; PROMOTERS
摘要	Long noncoding RNAs (lncRNAs) are increasingly being recognized as modulators of early embryonic development in mammals. However, they are seldom investigated in pigs. Here, to annotate full-length RNA transcripts, we performed annotation using a newly developed computational pipeline-an RNA-seq and small RNA-seq combined strategy-using our previously obtained RNA-seq and small RNA-seq data from porcine oocytes and zygotes. As evidenced by the length comparison, the frequency of the core promoter, and the polyadenylation signal motifs, the transcripts appear to be full-length. Furthermore, our strategy allowed the identification of a large number of endogenous retrovirus-associated lncRNAs (ERV-lncRNAs) and found that some of them were highly expressed in porcine zygotes, as compared to oocytes. Through the knockdown strategy, two ERV-lncRNAs (TCONS_00035465 and TCONS_00031520) were identified as playing potential roles in the early embryo development of pigs, laying a foundation for future research.
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-8021
EISSN	1664-8021
卷号	13 页码:1023041
DOI	10.3389/fgene.2022.1023041
页数	10
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000876513700001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36313467
SCOPUSEID	2-s2.0-85140711006
通讯作者地址	[Ding, Chunming]School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Xu, Chang]Department of Colorectal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Molecular Medicine;Genetics;Genetics (clinical)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167071
专题	检验医学院（生命科学学院、生物学实验教学中心）附属第一医院其他_瓯江实验室
通讯作者	Ding, Chunming; Xu, Chang
作者单位	1.School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Oujiang Laboratory,Zhejiang Lab for Regenerative Medicine,Vision and Brain Health,Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.Department of Colorectal Surgery,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）; 附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Yang, Xu,Ji, Jingzhang,Cui, Hongdi,et al. Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq.[J]. Frontiers in genetics,2022,13:1023041.
APA	Yang, Xu, Ji, Jingzhang, Cui, Hongdi, Zhao, Qi, Ding, Chunming, & Xu, Chang. (2022). Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq.. Frontiers in genetics, 13, 1023041.
MLA	Yang, Xu,et al."Functional evaluation of LTR-derived lncRNAs in porcine oocytes and zygotes with RNA-seq and small RNA-seq.".Frontiers in genetics 13(2022):1023041.