Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis

doi:10.1016/j.neulet.2015.12.047

科研成果详情

题名	Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis
作者	Sun, Jing 1; Ling, Zongxin 2; Wang, Fangyan3; Chen, Wenqian 4; Li, Haixiao 4; Jin, Jiangtao 4; Zhang, Huiqing 4; Pang, Mengqi 4; Yu, Junjie 4; Liu, Jiaming4
发表日期	2016-02-02
发表期刊	NEUROSCIENCE LETTERS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cerebral ischemic/reperfusion injury Anti-oxidative Anti-apoptosis Neuroprotective effects Butyrate
其他关键词	OXIDATIVE STRESS ; MICROBIOTA ; PREVENTS ; PROTEINS ; STROKE
摘要	Probiotics participate actively in the neuropsychiatric disorders. However, their roles on ischemic stroke remain unclear. This study aims to determine whether Clostridium butyricum (C. butyricum) could attenuate cerebral ischemia/reperfusion (I/R) injury and its possible mechanisms. Male ICR mice were intragastrically pretreated with C. butyricum for 2 successive weeks, and then subjected to cerebral I/R injury induced by the bilateral common carotid artery occlusion (BCCAO) for 20 min. After 24 h of the reperfusion, neurological deficit scores were evaluated. Histopathological changes of the hippocampus neurons were observed using Hematoxylin and eosin (H&E) and TUNEL staining. Malondialdehyde (MDA) contents and superoxide dismutase (SOD) activities in the brain were detected. The expression of Caspase-3, Bax and Bcl-2 were investigated by Western blot and immunohistochemistry analysis. The butyrate contents in the brain were determined. Our results showed that cerebral I/R injury led to neurological deficit, increased levels of Caspase-3 and Bax and decreased Bcl-2/Bax ratio. C. butyricum significantly improved neurological deficit, relieved histopathologic change, decreased MDA contents and increased SOD activities in the I/R injury mice. After C butyricum pretreatment, the expression of Caspase-3 and Bax were significantly decreased, the Bcl-2/Bax ratio was significantly increased, and butyrate contents in the brain were significantly increased. These findings suggested that C butyricum is able to exert neuroprotective effects against I/R injury mice through anti-oxidant and anti-apoptotic mechanisms, and reversing decrease of butyrate contents in the brain might be involved in its neuroprotection. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
资助项目	Zhejiang Medical Science and Technology Funds [2016136369]; Zhejiang Science and Technology Development Funds [2015C37010]
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0304-3940
EISSN	1872-7972
卷号	613 页码:30-35
DOI	10.1016/j.neulet.2015.12.047
页数	6
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000369463200006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	26733300
SCOPUSEID	2-s2.0-84952780110
通讯作者地址	[Liu, Jiaming]Department of Preventive Medicine,Wenzhou Medical University,1210 University Town,Wenzhou, Zhejiang,325035,China
Scopus学科分类	Neuroscience (all)
引用统计	被引频次：96[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16693
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院公共卫生学院_预防医学系基础医学院（机能实验教学中心）_病理学与病理生理学系
通讯作者	Liu, Jiaming
作者单位	1.Department of Neurology,The Second Affiliated Hospital of Wenzhou Medical University,109 College West Road,Wenzhou, Zhejiang,325027,China; 2.Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou, Zhejiang,310003,China; 3.Department of Pathophysiology,Wenzhou Medical University,1210 University Town,Wenzhou, Zhejiang,325035,China; 4.Department of Preventive Medicine,Wenzhou Medical University,1210 University Town,Wenzhou, Zhejiang,325035,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
通讯作者单位	预防医学系
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Sun, Jing,Ling, Zongxin,Wang, Fangyan,et al. Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis[J]. NEUROSCIENCE LETTERS,2016,613:30-35.
APA	Sun, Jing., Ling, Zongxin., Wang, Fangyan., Chen, Wenqian., Li, Haixiao., ... & Liu, Jiaming. (2016). Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis. NEUROSCIENCE LETTERS, 613, 30-35.
MLA	Sun, Jing,et al."Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis".NEUROSCIENCE LETTERS 613(2016):30-35.