Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola

doi:10.3389/fmicb.2022.990739

科研成果详情

题名	Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola
作者	Feng, Chunlin1,2; Gao, Mengdi2; Jiang, Weiyan1; Shi, Weina2; Li, Anqi2; Liu, Shuang2; Zhang, Lei2; Zhang, Xueya1; Li, Qiaoling1; Lin, Hailong1; Lu, Junwan 2,3; Li, Kewei2; Zhang, Hailin1; Hu, Yunliang1; Bao, Qiyu1,2,3; Lin, Xi1,2
发表日期	2022-09-13
发表期刊	Frontiers in microbiology 影响因子和分区
语种	英语
原始文献类型	Article
关键词	AadA33 Providencia vermicola aminoglycoside O-nucleotidyltransferase enzyme kinetics novel antimicrobial resistance gene
其他关键词	RESISTANCE GENES ; PROTEIN ; VISUALIZATION ; EMERGENCE ; OUTBREAK ; INTEGRON ; CASSETTE
摘要	A novel chromosome-encoded aminoglycoside O-nucleotidyltransferase AadA33 was identified in Providencia vermicola strain P13. The AadA33 shares the highest amino acid identity of 51.28% with the function characterized AadA31. Antibiotic susceptibility testing and enzyme kinetics analysis revealed that the function of AadA33 is to mediate spectinomycin and streptomycin resistance. The recombinant strain harboring aadA33 (pUCP20-aadA33/Escherichia coli DH5α) displayed >256- and 128-fold increases in the minimum inhibitory concentration levels to spectinomycin and streptomycin, respectively, compared with the control strains pUCP20/DH5α. Enzyme kinetic parameters manifested the substrate of AadA33 including spectinomycin and streptomycin, with k cat/K m of 3.28 × 104 (M-1 s-1) and 3.37 × 104 (M-1 s-1), respectively. Bioinformatics analysis revealed its structural mechanism of antimicrobial resistance, genetic context, and phylogenetic relationship with other aminoglycoside O-nucleotidyltransferases. This study of AadA33 contributed to understanding the function and resistance mechanism of aminoglycoside O-nucleotidyltransferase
资助项目	Science & Technology Project of Wenzhou City[N20210001,Y2020112];Natural Science Foundation of China[81973382];Zhejiang Provincial Natural Science Foundation of China[LQ17H190001,LY19C060002];
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	1664-302X
EISSN	1664-302X
卷号	13 页码:990739
DOI	10.3389/fmicb.2022.990739
页数	11
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000861179400001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36177473
SCOPUSEID	2-s2.0-85138909311
通讯作者地址	[Bao, Qiyu]The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China ; [Lin, Xi]The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Microbiology;Microbiology (medical)
TOP期刊	TOP期刊
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/166791
专题	附属第二医院检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Bao, Qiyu; Lin, Xi
作者单位	1.The Second Affiliated Hospital and Yuying Children’s Hospital,Wenzhou Medical University,Wenzhou,China; 2.Key Laboratory of Medical Genetics of Zhejiang Province,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,China; 3.Medical Molecular Biology Laboratory,School of Medicine,Jinhua Polytechnic,Jinhua,China
第一作者单位	附属第二医院; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Feng, Chunlin,Gao, Mengdi,Jiang, Weiyan,et al. Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola[J]. Frontiers in microbiology,2022,13:990739.
APA	Feng, Chunlin., Gao, Mengdi., Jiang, Weiyan., Shi, Weina., Li, Anqi., ... & Lin, Xi. (2022). Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola. Frontiers in microbiology, 13, 990739.
MLA	Feng, Chunlin,et al."Identification of a novel aminoglycoside O-nucleotidyltransferase AadA33 in Providencia vermicola".Frontiers in microbiology 13(2022):990739.