The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by binding mRNA and regulating its stability.

doi:10.1016/j.omto.2022.08.006

科研成果详情

题名	The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by binding mRNA and regulating its stability.
作者	Li, Xiaofei 1,2,3; Chen, Sixian 1,2,3; Wang, Xin 4; Zhang, Ruirui 1,2,3; Yang, Jialei 2; Xu, Haotian 1,2,3; He, Wanting 2; Lai, Mingshuang 2; Wu, Shuilian 4; Nan, Aruo1,2,3,4
发表日期	2022-09-15
发表期刊	Molecular therapy oncolytics 影响因子和分区
语种	英语
原始文献类型	Article
关键词	arsenic exposure circBRWD1 lung cancer mRNA stability
其他关键词	INDUCED MALIGNANT-TRANSFORMATION ; BEAS-2B CELLS ; C-MYC ; MECHANISMS ; PROGRESSION ; WORLDWIDE ; DAMAGE ; CYCLE
摘要	Multiple studies have indicated that circular RNAs (circRNAs) play a regulatory role in different stages of tumors by interacting with various molecules. With continuous in-depth research on the biological functions of circRNAs, increasing evidence has shown that circRNAs play important roles in carcinogenesis caused by environmental pollutants. However, the function and mechanism of circRNAs in arsenic exposure-induced lung cancer occurrence have not been reported. In this study, RNA sequencing and qPCR assays revealed that the expression of circBRWD1 was decreased in BEAS-2B-As cells and multiple lung cancer cell lines. Silencing circBRWD1 promoted cell viability and proliferation, inhibited cell apoptosis, and accelerated the G0/G1 phase transition in BEAS-2B-As cells; however, these functions were abrogated by circBRWD1 overexpression. Mechanistically, under arsenic exposure, expression of decreased circBRWD1 led to enhanced stability of the mRNA to which it directly binds (c-JUN, c-MYC, and CDK6 mRNA), increasing its expression. This mechanism promotes the malignant transformation of lung cells and ultimately leads to lung cancer. Our findings thus reveal the molecular mechanism of arsenic carcinogenesis.
资助项目	National Natural Science Foundation of China
出版者	CELL PRESS
出版地	CAMBRIDGE
ISSN	2372-7705
卷号	26 页码:399-412
DOI	10.1016/j.omto.2022.08.006
页数	14
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000859616000001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36159776
SCOPUSEID	2-s2.0-85137634047
通讯作者地址	[Nan, Aruo]Department of Toxicology,School of Public Health,Guangxi Medical University,Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Nanning,530021,China
Scopus学科分类	Molecular Medicine;Oncology;Cancer Research;Pharmacology (medical)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/166753
专题	检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Nan, Aruo
作者单位	1.Department of Toxicology,School of Public Health,Guangxi Medical University,Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Nanning,530021,China; 2.Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Guangxi Medical University,Nanning,530021,China; 3.Guangxi Key Laboratory of Environment and Health Research,Guangxi Medical University,Nanning,530021,China; 4.Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou,325035,China
推荐引用方式 GB/T 7714	Li, Xiaofei,Chen, Sixian,Wang, Xin,et al. The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by binding mRNA and regulating its stability.[J]. Molecular therapy oncolytics,2022,26:399-412.
APA	Li, Xiaofei., Chen, Sixian., Wang, Xin., Zhang, Ruirui., Yang, Jialei., ... & Nan, Aruo. (2022). The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by binding mRNA and regulating its stability.. Molecular therapy oncolytics, 26, 399-412.
MLA	Li, Xiaofei,et al."The pivotal regulatory factor circBRWD1 inhibits arsenic exposure-induced lung cancer occurrence by binding mRNA and regulating its stability.".Molecular therapy oncolytics 26(2022):399-412.