Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics

doi:10.3389/fpubh.2022.1007130

科研成果详情

题名	Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics
作者	Zhang, Tiemin 1; Ren, Guangxu 2,3,4; Zhou, Huanhuan 2,3,4; Qiang, Yu 2,3,4; Li, Jiaqi 2,3,4; Zhang, Yun 2,3,4; Li, Tingting 2,3,4; Zhou, Yunfei 2,3,4; Wang, Yuan 2,3,4; Lai, Xiuyi 2,3,4; Lei, Shen 2,3,4; Tan, Feng5; Liu, Rui 6; Li, Wenting 6; He, Jing 6; Zhao, Wei5; Zhu, Chuanlong 6; Lu, Gang 2,3,4,6
发表日期	2022-09-06
发表期刊	Frontiers in public health 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Enterocytozoon bieneusi Hainan genotype human zoonotic
其他关键词	GENOTYPES ; CRYPTOSPORIDIUM ; CHILDREN
摘要	Enterocytozoon bieneusi is a zoonotic pathogen commonly found in humans and animals all over the world. Here, we investigated the occurrence and genotype constitute of E. bieneusi among the individuals from Haikou city of Hainan, China. A total of 1,264 fecal samples of humans were collected, including 628 samples from patients with diarrhea (325 adults and 303 children) and 636 samples from the asymptomatic population (383 college students and 253 kindergarten children). E. bieneusi was detected using nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region. A phylogenetic tree was constructed using a neighbor-joining tree construction method. The overall prevalence of E. bieneusi was 3.7% (47/1,264), while it was 5.6% in the patients with diarrhea (5.8% in adults and 5.3% in children) and 1.9% in the asymptomatic population (2.9% in college students and 0.4% in kindergarten children). The prevalence of E. bieneusi in humans with diarrhea was significantly higher than that in the asymptomatic population (χ2 = 36.9; P < 0.05). A total of 28 genotypes were identified, including ten known genotypes: CHG2 (n = 3), CHG3 (n = 5), CHG5 (n = 10), CM21 (n = 1), EbpA (n = 1), EbpC (n = 1), PigEBITS4 (n = 1), PigEBITS7 (n = 1), SHR1 (n = 4), Type IV (n = 2), and 18 novel genotypes (HNH-1 to HNH-18; one each). All these genotypes were categorized into three groups, including group 1 (n = 6), group 2 (n = 14), and group 13 (n = 8). This was the first study on the identification of E. bieneusi among humans in Hainan, China. The correlation between E. bieneusi infection and diarrhea was observed. The high diversity and distinctive distribution of E. bieneusi genotypes found in this study reflected the unique epidemic genetic characteristics of E. bieneusi in humans living in Hainan
资助项目	National Natural Science Foundation of China [82060375, 81760378]; Research Project of Hainan Academician Innovation Platform [YSPTZX202004]; Major Science and Technology Program of Hainan Province [ZDKJ202003]; Hainan Talent Development Project [SRC200003]; Open Foundation of Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University [2020TTM004]; Hainan Province Clinical Medical Center
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	2296-2565
EISSN	2296-2565
卷号	10 页码:1007130
DOI	10.3389/fpubh.2022.1007130
页数	8
WOS类目	Public, Environmental & Occupational Health
WOS研究方向	Public, Environmental & Occupational Health
WOS记录号	WOS:000856047900001
收录类别	PUBMED ; SCIE ; SCOPUS ; SSCI
URL	查看原文
PubMed ID	36148343
SCOPUSEID	2-s2.0-85138343256
社科自定义期刊分类	E类
通讯作者地址	[Lu, Gang]Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,China ; [Zhao, Wei]Department of Parasitology,Wenzhou Medical University,Wenzhou,China ; [Zhu, Chuanlong]Department of Tropical Diseases,The Second Affiliated Hospital of Hainan Medical University,Haikou,China
Scopus学科分类	Public Health, Environmental and Occupational Health
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/166731
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Zhao, Wei; Zhu, Chuanlong; Lu, Gang
作者单位	1.Department of General Surgery,The Second Affiliated Hospital of Hainan Medical University,Haikou,China; 2.Key Laboratory of Tropical Translational Medicine of Ministry of Education,Hainan Medical University,Haikou,China; 3.Hainan Medical University,The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases,Hainan Medical University,Haikou,China; 4.Department of Pathogenic Biology,Hainan Medical University,Haikou,China; 5.Department of Parasitology,Wenzhou Medical University,Wenzhou,China; 6.Department of Tropical Diseases,The Second Affiliated Hospital of Hainan Medical University,Haikou,China
通讯作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
推荐引用方式 GB/T 7714	Zhang, Tiemin,Ren, Guangxu,Zhou, Huanhuan,et al. Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics[J]. Frontiers in public health,2022,10:1007130.
APA	Zhang, Tiemin., Ren, Guangxu., Zhou, Huanhuan., Qiang, Yu., Li, Jiaqi., ... & Lu, Gang. (2022). Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics. Frontiers in public health, 10, 1007130.
MLA	Zhang, Tiemin,et al."Molecular prevalence and genetic diversity analysis of Enterocytozoon bieneusi in humans in Hainan Province, China: High diversity and unique endemic genetic characteristics".Frontiers in public health 10(2022):1007130.