Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats

doi:10.1007/s11010-011-1023-y

科研成果详情

题名	Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats
作者	Xia, Xiaodong2; Hu, Xiaoguang2; Xu, Hui 2; Wu, Liqin2; Dai, Yuanrong2; Yang, Lei3; Xu, Zhengjie 1
发表日期	2012-01
发表期刊	MOLECULAR AND CELLULAR BIOCHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Phosphatidylinositol 3-kinase Asthma Nitric oxide Inducible nitric oxide synthase Airway inflammation
其他关键词	ALLERGIC AIRWAY INFLAMMATION ; FACTOR-KAPPA-B ; PHOSPHOINOSITIDE 3-KINASE ; HYPERRESPONSIVENESS ; ACTIVATION ; WORTMANNIN ; RESPONSES ; MICE ; AKT
摘要	Inducible nitric oxide synthase (iNOS) is known to produce nitric oxide (NO), which is a main contributor to asthmatic airway inflammation. Recent studies have shown that phosphatidylinositol 3-kinase (PI3K) is ubiquitously expressed in airway epithelial cells and its inhibition could relieve airway inflammation and hyperresponsiveness. This study aimed to explore the interaction of PI3K and NO signaling in allergic asthma. We investigated the effects of PI3K inhibitor wortmannin on iNOS expression in bronchiole epithelial cells and NO, IL-4 and IFN-gamma levels in lung tissues of asthmatic rat model, which was prepared by 10% OVA solution sensitization and 1% OVA aerosol challenge. Our results showed that the ratio of eosinophils to total cells in BALF, PI3K activity, NO and IL-4 levels in lung tissues was increased after OVA sensitization and challenge, but then was attenuated by the administration of wortmannin. In contrast, IFN-gamma level in lung tissues was decreased after OVA sensitization and challenge and increased after the administration of wortmannin. The expression of iNOS protein in bronchiole epithelial cells, iNOS mRNA level and iNOS activity in lung tissues was markedly upregulated after OVA sensitization and challenge, but the upregulation was significantly antagonized by wortmannin. Taken together, these data provide evidence that PI3K functions upstream to modulate iNOS/NO signaling, which then promotes the development of airway inflammation in asthmatic animal model. PI3K inhibitor wortmannin could lead to reduced iNOS expression and NO production, therefore inhibiting airway inflammatory responses.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2080466]
出版者	SPRINGER
出版地	DORDRECHT
ISSN	0300-8177
EISSN	1573-4919
卷号	359 期号:1-2 页码:293-299
DOI	10.1007/s11010-011-1023-y
页数	7
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000297174700030
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	21847581
SCOPUSEID	2-s2.0-83555164967
通讯作者地址	[Xu, Zhengjie]Wenzhou Med Coll, Dept Pathophysiol, Wenzhou 325027, Zhejiang, Peoples R China.
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1663
专题	基础医学院（机能实验教学中心）_病理学与病理生理学系附属第二医院_呼吸内科检验医学院（生命科学学院、生物学实验教学中心）_生物学实验教学中心
通讯作者	Xu, Zhengjie
作者单位	1.Wenzhou Med Coll, Dept Pathophysiol, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Dept Resp Med, Affiliated Hosp 2, Wenzhou 325027, Zhejiang, Peoples R China; 3.Wenzhou Med Coll, Ctr Lab Biol, Wenzhou 325027, Zhejiang, Peoples R China
第一作者单位	附属第二医院_呼吸内科
通讯作者单位	基础医学院（机能实验教学中心）_病理学与病理生理学系
第一作者的第一单位	附属第二医院_呼吸内科
推荐引用方式 GB/T 7714	Xia, Xiaodong,Hu, Xiaoguang,Xu, Hui,et al. Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2012,359(1-2):293-299.
APA	Xia, Xiaodong., Hu, Xiaoguang., Xu, Hui., Wu, Liqin., Dai, Yuanrong., ... & Xu, Zhengjie. (2012). Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats. MOLECULAR AND CELLULAR BIOCHEMISTRY, 359(1-2), 293-299.
MLA	Xia, Xiaodong,et al."Phosphatidylinositol 3-kinase inhibitor suppresses inducible nitric oxide synthase expression in bronchiole epithelial cells in asthmatic rats".MOLECULAR AND CELLULAR BIOCHEMISTRY 359.1-2(2012):293-299.