Gradient Elution LC-MS Determination of Dasatinib in Rat Plasma and Its Pharmacokinetic Study

doi:10.1556/AChrom.27.2015.1.7

科研成果详情

题名	Gradient Elution LC-MS Determination of Dasatinib in Rat Plasma and Its Pharmacokinetic Study
作者	Wen, C.1; Zhang, Q.2; He, Y.1; Deng, M.1; Wang, X.1; Ma, J.3
发表日期	2015-03
发表期刊	ACTA CHROMATOGRAPHICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	LC-ESI-MS rat plasma gradient elution dasatinib
其他关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; BCR-ABL ; KINASE INHIBITORS ; IMATINIB ; METABOLISM ; RESISTANCE ; BMS-354825 ; NILOTINIB
摘要	A sensitive and simple liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for determination of dasatinib in rat plasma using one-step protein precipitation was developed. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm x 150 mm, 5 mu m) column with methanol-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 488.2 for dasatinib and m/z 338.7 for the IS. Calibration plots were linear over the range of 10-1000 ng mL(-1) for dasatinib in rat plasma. Lower limit of quantification (LLOQ) for dasatinib was 10 ng mL(-1). Mean recovery of dasatinib from plasma was in the range 82.2%-93.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 8%. This developed method is successfully used in pharmacokinetic study of dasatinib in rats.
出版者	AKADEMIAI KIADO RT
出版地	BUDAPEST
ISSN	1233-2356
EISSN	2083-5736
卷号	27 期号:1 页码:81-91
DOI	10.1556/AChrom.27.2015.1.7
页数	11
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:000350994200007
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-84924777875
通讯作者地址	[Ma, J.]Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China.
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16596
专题	实验动物中心基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Ma, J.
作者单位	1.Wenzhou Med Coll, Lab Anim Ctr, Wenzhou 325035, Peoples R China; 2.Shanghai Inst Pharmaceut Ind, Shanghai 200437, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou 325035, Peoples R China
第一作者单位	实验动物中心
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	实验动物中心
推荐引用方式 GB/T 7714	Wen, C.,Zhang, Q.,He, Y.,et al. Gradient Elution LC-MS Determination of Dasatinib in Rat Plasma and Its Pharmacokinetic Study[J]. ACTA CHROMATOGRAPHICA,2015,27(1):81-91.
APA	Wen, C., Zhang, Q., He, Y., Deng, M., Wang, X., & Ma, J.. (2015). Gradient Elution LC-MS Determination of Dasatinib in Rat Plasma and Its Pharmacokinetic Study. ACTA CHROMATOGRAPHICA, 27(1), 81-91.
MLA	Wen, C.,et al."Gradient Elution LC-MS Determination of Dasatinib in Rat Plasma and Its Pharmacokinetic Study".ACTA CHROMATOGRAPHICA 27.1(2015):81-91.