Dl-3-n-butylphthalide prevents the disruption of blood-spinal cord barrier via inhibiting endoplasmic reticulum stress following spinal cord injury

doi:10.7150/ijbs.21107

科研成果详情

题名	Dl-3-n-butylphthalide prevents the disruption of blood-spinal cord barrier via inhibiting endoplasmic reticulum stress following spinal cord injury
作者	Zheng, Binbin1,2,3; Zhou, Yulong2,3; Zhang, Hongyu3; Yang, Guangyong1; Hong, Zhenghua1; Han, Dandan1; Wang, Qingqing 2; He, Zili2; Liu, Yanlong3; Wu, Fenzan 4; Zhang, Xie 5; Tong, Songlin 4; Xu, Huazi2; Xiao, Jian3
发表日期	2017-12-31
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Dl-3-n-butylphthalide Blood-spinal cord barrier Spinal cord injury Endocytoplasmic reticulum stress
其他关键词	IMPROVES FUNCTIONAL RECOVERY ; FOCAL CEREBRAL-ISCHEMIA ; ER-STRESS ; INDUCED APOPTOSIS ; CHIRAL 3-N-BUTYLPHTHALIDE ; OXIDATIVE STRESS ; BRAIN-BARRIER ; MODEL ; INFLAMMATION ; ACTIVATION
摘要	After spinal cord injury (SCI), the destruction of blood-spinal cord barrier (BSCB) is shown to accelerate gathering of noxious blood-derived components in the nervous system, leading to secondary neurodegenerative damages. SCI activates endoplasmic reticulum stress (ER stress), which is considered to evoke secondary damages of neurons and glia. Recent evidence indicates that Dl-3-n-butylphthalide (NBP) has the neuroprotective effect in ischaemic brain injury, but whether it has protective effects on SCI or not is largely unclear. Here, we show that NBP prevented BSCB disruption after SCI via inhibition of ER stress. Following a moderate contusion injury of the T9 level of spinal cord, NBP was administered by oral gavage and further treated once a day. NBP significantly attenuated BSCB permeability and breakdown of adherens junction (AJ) and tight junction (TJ) proteins, then improved locomotion recovery following SCI. The protective role of NBP on BSCB disruption is associated with the restrain of ER stress caused by SCI. Furthermore, NBP considerably constrained the expression of ER stress-associated proteins and degradation of TJ and AJ in human brain microvascular endothelial cells (HBMECs) treated with TG. In conclusion, our results indicate that ER stress is associated with the disruption of BSCB integrity after injury, NBP attenuates BSCB disruption via inhibiting ER stress and improve functional recovery following SCI.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81572237, 81572227, 81501953]; Technologies Science and technology Program of Zhejiang Province [2016C33107]; Public Technology Research Program of Zhejiang Province [LGF18H060011, GF18H060032]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [Q16H090023, Y14H170002]; Ningbo City Natural Science Foundation [2015A610213, 2015A610208]; Science and Technology Planning Program of Taizhou City [1702KY04]
出版者	IVYSPRING INT PUBL
出版地	LAKE HAVEN
ISSN	1449-2288
卷号	13 期号:12 页码:1520-1531
DOI	10.7150/ijbs.21107
页数	12
WOS类目	Biochemistry & Molecular Biology ; Biology
WOS研究方向	Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:000418365000006
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29230100
PMC记录号	PMC5723918
SCOPUSEID	2-s2.0-85036550056
通讯作者地址	[Xiao, Jian]School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Ecology, Evolution, Behavior and Systematics;Applied Microbiology and Biotechnology;Molecular Biology;Developmental Biology;Cell Biology
TOP期刊	TOP期刊
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16483
专题	药学院（分析测试中心）_分子药理研究与教学中心附属第二医院第二临床医学院、附属第二医院、育英儿童医院研究生工作部（研究生院）附属台州医院其他_附属台州医院（浙江省台州医院）其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Xiao, Jian
作者单位	1.Department of Orthopaedics,Taizhou Hospital,Wenzhou Medical University,Linhai,317000,China; 2.Key Laboratory of Orthopaedics of Zhejiang Province,Department of Orthopaedics,The Second Affiliated Hospital,Wenzhou Medical University,Wenzhou,325035,China; 3.Molecular Pharmacology Research Center,School of Pharmacy,Wenzhou Medical University,Wenzhou,325035,China; 4.Department of Neurosurgery,Affiliated Cixi People’s Hospital,Wenzhou Medical University,Ningbo,315300,China; 5.Department of Gastroenterology,Ningbo Medical Treatment Center Li Hui-li Hospital,Ningbo,315040,China
第一作者单位	附属台州医院（浙江省台州医院）; 附属台州医院; 附属第二医院; 药学院（分析测试中心）; 分子药理研究与教学中心
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	附属台州医院（浙江省台州医院）
推荐引用方式 GB/T 7714	Zheng, Binbin,Zhou, Yulong,Zhang, Hongyu,et al. Dl-3-n-butylphthalide prevents the disruption of blood-spinal cord barrier via inhibiting endoplasmic reticulum stress following spinal cord injury[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES,2017,13(12):1520-1531.
APA	Zheng, Binbin., Zhou, Yulong., Zhang, Hongyu., Yang, Guangyong., Hong, Zhenghua., ... & Xiao, Jian. (2017). Dl-3-n-butylphthalide prevents the disruption of blood-spinal cord barrier via inhibiting endoplasmic reticulum stress following spinal cord injury. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 13(12), 1520-1531.
MLA	Zheng, Binbin,et al."Dl-3-n-butylphthalide prevents the disruption of blood-spinal cord barrier via inhibiting endoplasmic reticulum stress following spinal cord injury".INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES 13.12(2017):1520-1531.