Determination of Deltaline in Rat Plasma by UPLC-MS/MS and its Application to Pharmacokinetics

科研成果详情

题名	Determination of Deltaline in Rat Plasma by UPLC-MS/MS and its Application to Pharmacokinetics
作者	Qiu, Erjie 1; Wang, Hongzhe 2; Yu, Yang 2; Song, Dezhen 2; Cai, Jinzhang3; Ye, Fenfen4
发表日期	2020
发表期刊	LATIN AMERICAN JOURNAL OF PHARMACY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	bioavailability deltaline pharmacokinetics rat UPLC-MS/MS
其他关键词	LC-ESI-MS ; CYTOCHROME-P450 ISOFORMS ; MOUSE-BLOOD
摘要	A sensitive and simple UPLC-MS/MS method was developed to detect deltaline in rat plasma, and its pharmacokinetics in rats was investigated. Precipitation method with acetonitrile was used for preparation samples. A BEH C18 column was used for chromatographic separation, mobile phase was acetonitrile-0.1% formic acid with gradient elution. Multiple reaction monitoring (MRM) mode with positive electrospray ionization (ESI)ionization was applied, 508.3 -> 110.0 and 285.1 -> 193.3 for deltaline and IS. Linearity of deltaline (0.5-500 ng/mL) in rat plasma was acceptable (r > 0.995), and the lower limit of quantification (LLOQ) was 0.5 ng/mL. Accuracy of method was between 91.2 and 109.3%, precision was lower than 13%, and matrix effect was between 103.7 and 108.7%. The UPLC-MS/MS method was successfully applied in the pharmacokinetics of deltaline in rats after oral and intravenous administration. The absolute bioavailability of the deltaline was 2.0% in rats.
资助项目	first batch of agricultural and community science and technology projects in Yinzhou District in 2019 [19]
出版者	COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES
出版地	LA PLATA
ISSN	0326-2383
卷号	39 期号:6 页码:1171-1175
页数	5
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000542662900016
收录类别	SCIE ; SCOPUS
URL	查看原文
SCOPUSEID	2-s2.0-85086878589
通讯作者地址	[Ye, Fenfen]Molecular Neuropharmacology Laboratory,School of Optometry and Ophthalmology and Eye Hospital,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Pharmaceutical Science
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16465
专题	实验动物中心第二临床医学院、附属第二医院、育英儿童医院眼视光学院（生物医学工程学院）、附属眼视光医院眼视光学院（生物医学工程学院）、附属眼视光医院_分子生物学实验室
通讯作者	Ye, Fenfen
作者单位	1.Department of Clinical Pharmacy,Ningbo YinZhou No. 2 Hospital,Ningbo,315400,China; 2.Laboratory Animal Centre,Wenzhou Medical University,Wenzhou,325035,China; 3.The Second Affiliated & Yuying Children’s Hospital Wenzhou Medical University,Wenzhou,325000,China; 4.Molecular Neuropharmacology Laboratory,School of Optometry and Ophthalmology and Eye Hospital,Wenzhou Medical University,Wenzhou,325000,China
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Qiu, Erjie,Wang, Hongzhe,Yu, Yang,et al. Determination of Deltaline in Rat Plasma by UPLC-MS/MS and its Application to Pharmacokinetics[J]. LATIN AMERICAN JOURNAL OF PHARMACY,2020,39(6):1171-1175.
APA	Qiu, Erjie, Wang, Hongzhe, Yu, Yang, Song, Dezhen, Cai, Jinzhang, & Ye, Fenfen. (2020). Determination of Deltaline in Rat Plasma by UPLC-MS/MS and its Application to Pharmacokinetics. LATIN AMERICAN JOURNAL OF PHARMACY, 39(6), 1171-1175.
MLA	Qiu, Erjie,et al."Determination of Deltaline in Rat Plasma by UPLC-MS/MS and its Application to Pharmacokinetics".LATIN AMERICAN JOURNAL OF PHARMACY 39.6(2020):1171-1175.