Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis

doi:10.1016/j.gene.2020.144939

科研成果详情

题名	Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis
作者	Li, Chenlu 1; Luo, Jing 1; Xu, Xin 2; Zhou, Zihao 2; Ying, Senhong 2; Liao, Xin 2; Wu, Keke 3
发表日期	2020-10-05
发表期刊	GENE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Osteoarthritis Single-cell sequencing Cell cluster analysis Functional enrichment analysis RT-qPCR
其他关键词	CHONDROCYTES ; KNEE ; PROGRESSION ; EXPRESSION ; MIGRATION ; FIBROSIS ; DISEASE ; GROWTH ; GENES
摘要	Osteoarthritis (OA) is a chronic degenerative change with high incidence and leads to a lower quality of life and a larger socioeconomic burden. This study aimed to explore potential crucial genes and pathways associated with OA that can be used as potential biomarkers for early treatment. Single-cell gene expression profile of 1464 chondrocytes and 192 fibroblasts in OA were downloaded from the public database (GSE104782 and GSE109449) for subsequent analysis. A total of eight clusters in chondrocytes and three clusters in fibroblasts of OA were identified using the Seurat pipeline and the SingleR package for cell-type annotation. Moreover, 44 common marker-genes between fibroblastic-like chondrocytes and fibroblasts were identified and the focal adhesions pathway was further identified as a significant potential mechanism of OA via functional enrichment analysis. Further, the reverse transcription quantitative real-time PCR (RT-qPCR) experiments at tissue's and cellular level confirmed that two key marker-genes (COL6A3 and ACTG1) might participate in the progression of OA. Summarily, we inferred that chondrocytes in OA might up-regulate the expression of COL6A3 and ACTG1 to complete fibroblasts transformation through the focal adhesion pathway. These findings are expected to gain a further insight into the development of OA fibrosis process and provide a promising target for treatment for early OA.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81700062]; Natural Science Foundation of Zhejiang Province, ChinaNatural Science Foundation of Zhejiang Province [LQ16H010003]; Zhejiang Xinmiao Talents Program, China [2019R413082]; General scientific projects of Zhejiang Education Department, China [Y201942208]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	0378-1119
EISSN	1879-0038
卷号	757 页码:144939
DOI	10.1016/j.gene.2020.144939
页数	11
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000564684100011
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	32640306
SCOPUSEID	2-s2.0-85087511134
通讯作者地址	[Wu, Keke]Wenzhou Center for Disease Control and Prevention,Wenzhou,China
Scopus学科分类	Genetics
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16459
专题	附属第一医院_风湿免疫科基因组医学研究院
通讯作者	Wu, Keke
作者单位	1.Rheumatology Department,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Institute of Genomic Medicine,Wenzhou Medical University,Wenzhou,China; 3.Wenzhou Center for Disease Control and Prevention,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Li, Chenlu,Luo, Jing,Xu, Xin,et al. Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis[J]. GENE,2020,757:144939.
APA	Li, Chenlu., Luo, Jing., Xu, Xin., Zhou, Zihao., Ying, Senhong., ... & Wu, Keke. (2020). Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis. GENE, 757, 144939.
MLA	Li, Chenlu,et al."Single cell sequencing revealed the underlying pathogenesis of the development of osteoarthritis".GENE 757(2020):144939.