Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart

doi:10.1007/s00246-017-1569-x

科研成果详情

题名	Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart
作者	Geng, Zhimin1,2; Wang, Jue3; Pan, Lulu 1; Li, Ming 4; Zhang, Jitai 4; Cai, Xueli5; Chu, Maoping1
发表日期	2017-04
发表期刊	PEDIATRIC CARDIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Microarray Bioinformatics Human heart Fetal Adult
其他关键词	NEUREGULIN RECEPTOR ; CARDIAC DEVELOPMENT ; CARDIOMYOCYTES ; PROLIFERATION ; GROWTH
摘要	Although many changes have been discovered during heart maturation, the genetic mechanisms involved in the changes between immature and mature myocardium have only been partially elucidated. Here, gene expression profile changed between the human fetal and adult heart was characterized. A human microarray was applied to define the gene expression signatures of the fetal (13-17 weeks of gestation, n = 4) and adult hearts (30-40 years old, n = 4). Gene ontology analyses, pathway analyses, gene set enrichment analyses, and signal transduction network were performed to predict the function of the differentially expressed genes. Ten mRNAs were confirmed by quantificational real-time polymerase chain reaction. 5547 mRNAs were found to be significantly differentially expressed. Cell cycle was the most enriched pathway in the down-regulated genes. EFGR, IGF1R, and ITGB1 play a central role in the regulation of heart development. EGFR, IGF1R, and FGFR2 were the core genes regulating cardiac cell proliferation. The quantificational real-time polymerase chain reaction results were concordant with the microarray data. Our data identified the transcriptional regulation of heart development in the second trimester and the potential regulators that play a prominent role in the regulation of heart development and cardiac cells proliferation.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670255]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H020008, LY13H020005]; Science and Technology Department of Zhejiang Province [2015C33163]; Scientific Research Foundation of Wenzhou [Y20150015]; Zhejiang Provincial Medical and Health Science and Technology plan [2016KYB197]
出版者	SPRINGER
出版地	NEW YORK
ISSN	0172-0643
EISSN	1432-1971
卷号	38 期号:4 页码:700-706
DOI	10.1007/s00246-017-1569-x
页数	7
WOS类目	Cardiac & Cardiovascular Systems ; Pediatrics
WOS研究方向	Cardiovascular System & Cardiology ; Pediatrics
WOS记录号	WOS:000399219800008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28331934
SCOPUSEID	2-s2.0-85015909824
通讯作者地址	[Chu, Maoping]Children’s Heart Center,The Second Affiliated Hospital & Yuying Children’s Hospital,Institute of Cardiovascular Development and Translational Medicine,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Pediatrics, Perinatology and Child Health;Cardiology and Cardiovascular Medicine
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16308
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院附属第一医院基础医学院（机能实验教学中心）_基础医学研究中心_心脏再生医学研究所
通讯作者	Chu, Maoping
作者单位	1.Children’s Heart Center,The Second Affiliated Hospital & Yuying Children’s Hospital,Institute of Cardiovascular Development and Translational Medicine,Wenzhou Medical University,Wenzhou,China; 2.Tianjin Children Hospital,Tianjin,China; 3.Department of Cardiac Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Cardiac Regeneration Research Institute,Wenzhou Medical University,Wenzhou,China; 5.Department of Cardiolgy,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第二医院
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Geng, Zhimin,Wang, Jue,Pan, Lulu,et al. Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart[J]. PEDIATRIC CARDIOLOGY,2017,38(4):700-706.
APA	Geng, Zhimin., Wang, Jue., Pan, Lulu., Li, Ming., Zhang, Jitai., ... & Chu, Maoping. (2017). Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart. PEDIATRIC CARDIOLOGY, 38(4), 700-706.
MLA	Geng, Zhimin,et al."Microarray Analysis of Differential Gene Expression Profile Between Human Fetal and Adult Heart".PEDIATRIC CARDIOLOGY 38.4(2017):700-706.