Microarray analysis of long non-coding RNA expression profiles in Marfan syndrome

doi:10.3892/etm.2020.9093

科研成果详情

题名	Microarray analysis of long non-coding RNA expression profiles in Marfan syndrome
作者	Gu, Lizhong1,2; Ni, Jiangwei 3; Sheng, Sunpeng 4; Zhao, Kaixiang 5; Sun, Chengchao4; Wang, Jue4
发表日期	2020-10
发表期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	marfan syndrome long noncoding RNA microarray bioinformatics analyses
其他关键词	THORACIC AORTIC-ANEURYSMS ; CELLS ; INSIGHTS ; MECHANISMS ; RESPONSES ; CANCER ; ROLES
摘要	Long non-coding RNAs (lncRNAs) serve a crucial role in every aspect of cell biological functions as well as in a variety of diseases, including cardiovascular disease, cancer and nervous system disease. However, the differential expression profiles of lncRNAs in Marfan syndrome (MFS) have not been reported. The aim of the present study was to identify potential target genes behind the pathogenesis of MFS by analyzing microarray profiles of lncRNA in aortic tissues from individuals with MFS and normal aortas (NA). The differentially expressed lncRNA profiles between MFS (n=3) and NA (n=4) tissues were analyzed using microarrays. Bioinformatics analyses were used to further investigate the candidate lncRNAs. Reverse transcription-quantitative (RT-qPCR) was applied to validate the results. In total, the present study identified 294 lncRNAs (245 upregulated and 49 downregulated) and 644 mRNAs (455 upregulated and 189 downregulated) which were differential expressed between MFS and NA tissues (fold change >= 1.5; P<0.05). Gene Ontology enrichment analysis indicated that the differentially expressed mRNAs were involved in cell adhesion, elastic fiber assembly, extracellular matrix (ECM) organization, the response to virus and the inflammatory response. Kyoto Encyclopedia of Gene and Genomes pathway analysis indicated that the differentially expressed mRNAs were mainly associated with focal adhesion, the ECM-receptor interaction, the mitogen-activated protein kinase signaling pathway and the tumor necrosis factor signaling pathway. The lncRNA-mRNA coexpression network analysis further elucidated the interaction between the lncRNAs and mRNAs. A total of five lncRNAs (uc003jka.1, uc003jox.1, X-inactive specific transcript, linc-lysophosphatidic acid receptor 1 and linc-peptidylprolyl isomerase domain and WD repeat containing 1) with the highest degree of coexpression were selected and confirmed using RT-qPCR. In the present study, expression profiles of lncRNA and mRNA in MFS were revealed using microarray analysis. These results provided novel candidates for further investigation of the molecular mechanisms and effective targeted therapies for MFS.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670255]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H020008]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1792-0981
EISSN	1792-1015
卷号	20 期号:4 页码:3615-3624
DOI	10.3892/etm.2020.9093
页数	10
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000575004100088
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	32855713
PMC记录号	PMC7444390
通讯作者地址	[Wang, Jue]Wenzhou Med Univ, Dept Cardiac Surg, Affiliated Hosp 1, Nanbaixiang St, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16218
专题	附属第二医院_心胸外科附属第一医院第二临床医学院，附属第二医院、育英儿童医院附属第一医院_心胸外科
通讯作者	Wang, Jue
作者单位	1.Wenzhou Med Univ, Dept Cardiothorac Surg, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Dept Thorac Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Dept Cardiac Surg, Affiliated Hosp 1, Nanbaixiang St, Wenzhou 325000, Zhejiang, Peoples R China; 5.Zhejiang Hosp, Dept Cardiothorac Surg, Hangzhou 310000, Zhejiang, Peoples R China
第一作者单位	附属第二医院_心胸外科; 第二临床医学院，附属第二医院、育英儿童医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第二医院_心胸外科
推荐引用方式 GB/T 7714	Gu, Lizhong,Ni, Jiangwei,Sheng, Sunpeng,et al. Microarray analysis of long non-coding RNA expression profiles in Marfan syndrome[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2020,20(4):3615-3624.
APA	Gu, Lizhong, Ni, Jiangwei, Sheng, Sunpeng, Zhao, Kaixiang, Sun, Chengchao, & Wang, Jue. (2020). Microarray analysis of long non-coding RNA expression profiles in Marfan syndrome. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 20(4), 3615-3624.
MLA	Gu, Lizhong,et al."Microarray analysis of long non-coding RNA expression profiles in Marfan syndrome".EXPERIMENTAL AND THERAPEUTIC MEDICINE 20.4(2020):3615-3624.