Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis

doi:10.1016/j.diagmicrobio.2017.02.001

科研成果详情

题名	Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis
作者	Zhang, Liang 1; Guo, Gangqiang2; Xu, Jianfeng 1; Sun, Xiangwei1; Chen, Wenjing 1; Jin, Jinji 1; Hu, Changyuan1; Zhang, Peichen1; Shen, Xian1,3; Xue, Xiangyang2
发表日期	2017-05
发表期刊	DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Quantitative real-time PCR Immunohistochemistry Virus and cancer Metastasis
其他关键词	COLORECTAL-CANCER ; GLIOBLASTOMA-MULTIFORME ; INFECTION ; CELLS ; DISEASE ; PREVALENCE ; ACTIVATION ; EXPRESSION ; ONCOGENES ; PROTEINS
摘要	Increasing evidence suggests that human cytomegalovirus (HCMV) is associated with many human malignancies. However, its prevalence in gastric cancer (GC) and clinical association remain unknown. HCMV IgG and IgM antibodies in the sera of 80 GC patients and 80 healthy controls were detected using a microparticle enzyme immunoassay. The prevalence of HCMV UL47, UL55, UL56, and UL77 genes among 102 GC tumor tissues and adjacent normal specimens was measured by polymerase chain reaction (PCR) or nested PCR. Quantitative real-time PCR (Q-PCR) was used to determine viral load. Virus localization in neoplastic tissues was determined by immunohistochemistry. No significant difference of HCMV IgG and IgM seropositivity was found between GC patients and the healthy group. However, the overall HCMV DNA positivity rate was significantly higher in GC cancerous tissue compared with in paired normal tissue (P < 0.01). HCMV infection was mainly localized in the tumorous epithelium. Q-PCR in HCMV-positive specimens indicated that the viral copy number was notably higher in GC tissues than in adjacent normal specimens (P < 0.001). Clinical statistical analysis indicated that HCMV load in GC tumor tissue was positively associated with lymphatic metastasis (P = 0.043), the area under the receiver operating characteristic (ROC) curve was 0.6638. Our data clearly provide the prevalence of HCMV in GC patients. We conclude that HCMV infection in malignant tissues might be associated with carcinogenesis or progression of GC and possibly relates to lymphatic metastasis. (C) 2017 Elsevier Inc. All rights reserved.
资助项目	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81472308, 81001343, 31470891]; Projects of Wenzhou technology bureau [Y20150054, Y20150057]
出版者	ELSEVIER SCIENCE INC
出版地	NEW YORK
ISSN	0732-8893
EISSN	1879-0070
卷号	88 期号:1 页码:62-68
DOI	10.1016/j.diagmicrobio.2017.02.001
页数	7
WOS类目	Infectious Diseases ; Microbiology
WOS研究方向	Infectious Diseases ; Microbiology
WOS记录号	WOS:000400541600016
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28238538
SCOPUSEID	2-s2.0-85013643451
通讯作者地址	[Shen, Xian]Department of Gastrointestinal Surgery,the First Affiliated Hospital of Wenzhou Medical University,Ouhai District,Wenzhou,325000,China
Scopus学科分类	Microbiology (medical);Infectious Diseases
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1602
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Shen, Xian
作者单位	1.Department of Gastrointestinal Surgery,the First Affiliated Hospital of Wenzhou Medical University,Ouhai District,Wenzhou,325000,China; 2.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Institute of tropical medicine,Wenzhou Medical University,Ouhai District,Wenzhou,325000,China; 3.Department of Gastrointestinal Surgery,the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University,Luchen District,Wenzhou,325027,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhang, Liang,Guo, Gangqiang,Xu, Jianfeng,et al. Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis[J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE,2017,88(1):62-68.
APA	Zhang, Liang., Guo, Gangqiang., Xu, Jianfeng., Sun, Xiangwei., Chen, Wenjing., ... & Xue, Xiangyang. (2017). Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 88(1), 62-68.
MLA	Zhang, Liang,et al."Human cytomegalovirus detection in gastric cancer and its possible association with lymphatic metastasis".DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 88.1(2017):62-68.