miR-138 suppresses the proliferation, metastasis and autophagy of non-small cell lung cancer by targeting Sirt1

doi:10.3892/or.2017.5619

科研成果详情

题名	miR-138 suppresses the proliferation, metastasis and autophagy of non-small cell lung cancer by targeting Sirt1
作者	Ye, Zaiting 1; Fang, Bingmu 1; Pan, Jiongwei 1; Zhang, Ning 1; Huang, Jinwei 2; Xie, Congying3; Lou, Tianzheng 1; Cao, Zhuo 1,4
发表日期	2017-06
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	miR-138 non-small cell lung cancer autophagy mTOR signaling pathway
其他关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; AMPK/MTOR PATHWAY ; TUMOR-SUPPRESSOR ; CERVICAL-CANCER ; GASTRIC-CANCER ; UP-REGULATION ; DEACETYLASE ; PROMOTES ; SURVIVAL ; PROTEIN
摘要	The present study determined the role and mechanism of miR-138 in non-small cell lung cancer (NSCLC). In total, 45 freshly resected clinical NSCLC tissues were collected. The expression of miR-138 in tissues and cell lines were determined by real-time quantitative PCR. miR-138 mimics were transfected into A549 and Calu-3 cells in vitro, and then the effects of miR-138 on lung cancer cell proliferation, cell cycle, invasion and metastasis were investigated by CCK-8 assay, Transwell and flow cytometry, respectively. The protein expression of the potential target gene Sirt1 in lung cancer cells were determined by western blot analysis. Dual-Luciferase reporter assay was performed to further confirm whether Sirt1 was the target gene of miR-138. The expression of miR-138 was significantly lower in lung cancer tissues and was negatively correlated to the differentiation degree and lymph node metastasis of lung cancer. In vitro experiment results showed that miR-138 inhibited lung cancer cell proliferation, invasion and migration. It was verified that miR-138 could downregulate Sirtl protein expression, inhibit epithelial-mesenchymal transition (EMT), decrease the activity of AMPK signaling pathway and elevate mTOR phosphorylation level. Dual-Luciferase reporter assay demonstrated that miR-138 could directly regulate Sirt1. Downregulation of Sirtl alone can also cause the same molecular and biological function changes. Western blot analysis and confocal microscopy results indicated that overexpression of miR-138 or interference of Sirtl expression could inhibit lung cancer cell autophagy activity possibly through AMPK-mTOR signaling pathway. miR-138 plays a tumor suppressor function in lung cancer. It may inhibit the proliferation, invasion and migration of lung cancer through downregulation of Sirtl expression and activation of cell autophagy. The downregulation of miR-138 is closely related to the development of lung cancer.
资助项目	Program of Science Technology Department of Zhejiang Province [2016C33SAB0009]; Program of Science and Technology Bureau of Lishui City [2014JYZB12]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	37 期号:6 页码:3244-3252
DOI	10.3892/or.2017.5619
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000402692300011
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28498463
PMC记录号	PMC5442395
SCOPUSEID	2-s2.0-85019559304
通讯作者地址	[Lou, Tianzheng]Sixth Affiliated Hospital of Wenzhou Medical University,Lishui People's Hospitlal,Lishui, Zhejiang,323000,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：44[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16021
专题	其他_附属第六医院（丽水市人民医院）附属第一医院第二临床医学院，附属第二医院、育英儿童医院_麻醉学_ICU
通讯作者	Lou, Tianzheng
作者单位	1.Sixth Affiliated Hospital of Wenzhou Medical University,Lishui People's Hospitlal,Lishui, Zhejiang,323000,China; 2.Central Hospital of Lishui City,Lishui, Zhejiang,323000,China; 3.First Affiliated Hospital of Wenzhou Medical University,Lishui, Zhejiang,323000,China; 4.Department of ICU,Sixth Affiliated Hospital of Wenzhou Medical University,Lishui People's Hospital,Lishui, Zhejiang,323000,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Ye, Zaiting,Fang, Bingmu,Pan, Jiongwei,et al. miR-138 suppresses the proliferation, metastasis and autophagy of non-small cell lung cancer by targeting Sirt1[J]. ONCOLOGY REPORTS,2017,37(6):3244-3252.
APA	Ye, Zaiting., Fang, Bingmu., Pan, Jiongwei., Zhang, Ning., Huang, Jinwei., ... & Cao, Zhuo. (2017). miR-138 suppresses the proliferation, metastasis and autophagy of non-small cell lung cancer by targeting Sirt1. ONCOLOGY REPORTS, 37(6), 3244-3252.
MLA	Ye, Zaiting,et al."miR-138 suppresses the proliferation, metastasis and autophagy of non-small cell lung cancer by targeting Sirt1".ONCOLOGY REPORTS 37.6(2017):3244-3252.