Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis

doi:10.3892/or.2012.1634

科研成果详情

题名	Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis
作者	Zheng, Shu-Rong1; Guo, Gui-Long1; Zhang, Wei 1; Huang, Guan-Li1; Hu, Xiao-Qu1; Zhu, Jin 2; Huang, Qi-Di1; You, Jie1; Zhang, Xiao-Hua 1
发表日期	2012-04
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	breast neoplasms microRNAs microRNA-155 clinicopathological features antisense oligonucleotide viability apoptosis
其他关键词	MICRORNA EXPRESSION ; PROGNOSTIC-FACTORS ; GROWTH ; CARCINOMA ; TARGETS ; INFLAMMATION ; SURVIVAL ; GENE ; LYMPHOMAS ; PATHWAYS
摘要	Accumulating evidence shows that mircroRNAs (miRNAs) play a vital role in tumorigenesis. miR-155 is one of the most multifunctional miRNAs whose overexpression has been found to be associated with different types of cancer including breast cancer. To further determine the potential involvement of miR-155 in breast cancer, we evaluated the expression levels of miR-155 by real-time PCR and correlated the results with clinicopathological features. Matched non-tumor and tumor tissues of 42 infiltrating ductal carcinomas and 3 infiltrating lobular carcinomas were analyzed for miR-155 expression by real-time PCR. Further, we used an antisense technique to inhibit miR-155 expression in vitro. WST-8 test was performed to evaluate cell viability and apoptosis assay was used to investigate the effect of the miR-155 antisense oligonucleotide (miR-155 ASO) on HS578T cell death. The expression levels of miR-155 were significantly higher in tumor tissues than the levels in matched non-tumor tissues (P<0.001). Up-regulated miR-155 expression was associated with lymph node positivity (P=0.034), higher proliferation index (Ki-67 >10%) (P=0.019) and advanced breast cancer TNM clinical stage (P=0.002). Interestingly, we next found that miR-155 expression levels had close relations with ER status (P=0.041) and PR status (P=0.029). Transfection efficiency detected by flow cytometry was higher than 70%, the WST-8 test showed that viability of HS578T cells was greatly reduced after transfection with miR-155 ASO compared with the scramble (SCR) group or the liposome group. The Annexin V-FITC/PI assay also indicated that transfection with miR-155 ASO promoted apoptosis.
资助项目	Wenzhou Science and Technology Bureau [Y20080081, Y20100008]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	27 期号:4 页码:1149-1155
DOI	10.3892/or.2012.1634
页数	7
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000301757600036
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22245916
PMC记录号	PMC3583512
SCOPUSEID	2-s2.0-84863043199
通讯作者地址	[Guo, Gui-Long]Wenzhou Med Coll, Affiliated Hosp 1, Dept Oncol, 2 Fu Xue Rd, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15718
专题	附属第一医院_肿瘤内科
通讯作者	Guo, Gui-Long
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Oncol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Jiaxing Matern & Child Hlth Care Hosp, Dept Breast Surg, Jiaxing 314000, Zhejiang, Peoples R China
第一作者单位	附属第一医院_肿瘤内科
通讯作者单位	附属第一医院_肿瘤内科
第一作者的第一单位	附属第一医院_肿瘤内科
推荐引用方式 GB/T 7714	Zheng, Shu-Rong,Guo, Gui-Long,Zhang, Wei,et al. Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis[J]. ONCOLOGY REPORTS,2012,27(4):1149-1155.
APA	Zheng, Shu-Rong., Guo, Gui-Long., Zhang, Wei., Huang, Guan-Li., Hu, Xiao-Qu., ... & Zhang, Xiao-Hua. (2012). Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis. ONCOLOGY REPORTS, 27(4), 1149-1155.
MLA	Zheng, Shu-Rong,et al."Clinical significance of miR-155 expression in breast cancer and effects of miR-155 ASO on cell viability and apoptosis".ONCOLOGY REPORTS 27.4(2012):1149-1155.