A method for rapid and sensitive negative staining of proteins in SDS-PAGE using 2 ',7 '-dichlorofluorescein

doi:10.1002/pmic.201600346

科研成果详情

题名	A method for rapid and sensitive negative staining of proteins in SDS-PAGE using 2 ',7 '-dichlorofluorescein
作者	Zhang, Yuan1; Yu, Dongdong 1; Dai, Jiangbo 1; Wang, Xu 1,2; Zhu, Zhongxin 1,2; Huang, Yewei 1; Sun, Jia 1; Chen, Zhiwei1; Jin, Litai1,2; Wang, Yang3
发表日期	2017-06-17
发表期刊	PROTEOMICS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	2 ',7 '-Dichlorofluorescein Eosin Y Imidazole-zinc Negative stain Proteomics
其他关键词	SYPRO-RUBY ; POLYACRYLAMIDE GELS ; BLUE
摘要	We report here a rapid and sensitive technique for negative visualization of protein in 1D and 2D SDS-PAGE by using 2 ', 7 '-dichlorofluorescein (DCF), which appeared as transparent and colorless bands in an opaque gel matrix background. For DCF stain, down to 0.1-0.2ng protein could be easily visualized within 7min by only two steps, and the staining is fourfold more sensitive than that of Eosin Y (EY) negative stain and glutaraldehyde (GA) silver stain, and eightfold more sensitive than that of the commonly used imidazole-zinc (IZ) negative stain. Furthermore, DCF stain provided good reproducibility, linearity, and MS compatibility compared with those of IZ stain. In addition, the potential staining mechanism was investigated by colorimetric experiment and molecular docking, and the results demonstrated that the interaction between DCF and protein occurs mainly via van der waals force, electrostatic interaction, and hydrogen bonding.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81500295]; National Undergraduate Training Program for Innovation and Entrepreneurship [201510343015]; Public Projects of Zhejiang Province [2016C37010, 2015C33122]; Qianjiang talents Program [QJD1502030]; Young Talent Cultivation Project of Zhejiang Association for Science and Technology [2016YCGC001]; Scientific Research Fund of Zhejiang Provincial Education Department [Y201534202]; Technology Program of Wen-zhou [Y20150010]
出版者	WILEY
出版地	HOBOKEN
ISSN	1615-9853
EISSN	1615-9861
卷号	17 期号:12
DOI	10.1002/pmic.201600346
页数	6
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000403899900009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	28467633
SCOPUSEID	2-s2.0-85020173812
通讯作者地址	[Jin, Litai]School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Biochemistry;Molecular Biology
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15649
专题	药学院（分析测试中心）基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Jin, Litai
作者单位	1.School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China; 2.Wenzhou Undersun Biotechnology Co. Ltd.,Wenzhou,China; 3.Institute of neuroscience,Department of histology and embryology,Wenzhou Medical University,Wenzhou,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Zhang, Yuan,Yu, Dongdong,Dai, Jiangbo,et al. A method for rapid and sensitive negative staining of proteins in SDS-PAGE using 2 ',7 '-dichlorofluorescein[J]. PROTEOMICS,2017,17(12).
APA	Zhang, Yuan., Yu, Dongdong., Dai, Jiangbo., Wang, Xu., Zhu, Zhongxin., ... & Wang, Yang. (2017). A method for rapid and sensitive negative staining of proteins in SDS-PAGE using 2 ',7 '-dichlorofluorescein. PROTEOMICS, 17(12).
MLA	Zhang, Yuan,et al."A method for rapid and sensitive negative staining of proteins in SDS-PAGE using 2 ',7 '-dichlorofluorescein".PROTEOMICS 17.12(2017).