Discoveries in Pancreatic Physiology and Disease Biology Using Single-Cell RNA Sequencing

doi:10.3389/fcell.2021.732776

科研成果详情

名称	Discoveries in Pancreatic Physiology and Disease Biology Using Single-Cell RNA Sequencing
作者	Fu, Haotian 1,2; Sun, Hongwei1; Kong, Hongru1; Lou, Bin 3; Chen, Hao4; Zhou, Yilin 5; Huang, Chaohao1; Qin, Lei 6; Shan, Yunfeng1,2; Dai, Shengjie1,6
发表期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
语种	英语
原始文献类型	Review
关键词	single-cell single-cell RNA sequencing pancreas diabetes pancreatic cancer
其他关键词	CIRCULATING TUMOR-CELLS ; BETA-CELL ; INSULIN-RESISTANCE ; ISLET ARCHITECTURE ; GENE-EXPRESSION ; STELLATE CELLS ; CHARACTERISTIC FEATURES ; CANCER PROGRESSION ; HETEROGENEITY ; PATHWAY
摘要	Transcriptome analysis is used to study gene expression in human tissues. It can promote the discovery of new therapeutic targets for related diseases by characterizing the endocrine function of pancreatic physiology and pathology, as well as the gene expression of pancreatic tumors. Compared to whole-tissue RNA sequencing, single-cell RNA sequencing (scRNA-seq) can detect transcriptional activity within a single cell. The scRNA-seq had an invaluable contribution to discovering previously unknown cell subtypes in normal and diseased pancreases, studying the functional role of rare islet cells, and studying various types of cells in diabetes as well as cancer. Here, we review the recent in vitro and in vivo advances in understanding the pancreatic physiology and pathology associated with single-cell sequencing technology, which may provide new insights into treatment strategy optimization for diabetes and pancreatic cancer.
资助项目	exploration project Q and Y of Zhejiang Natural Sciences Foundation [LQ20H160014, LY20H160012]; Zhejiang Medical and Health Science and Technology Plan Project [2019RC210]; College-Level Incubation Project [FHY2019019]; National Natural Sciences Foundation of China [81770630]
出版者	FRONTIERS MEDIA SA
出版地	LAUSANNE
ISSN	2296-634X
卷号	9
DOI	10.3389/fcell.2021.732776
页数	18
WOS类目	Cell Biology ; Developmental Biology
WOS研究方向	Cell Biology ; Developmental Biology
WOS记录号	WOS:000751992700001
收录类别	SCIE
发布日期	2022-01-24
PubMed ID	35141228
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	其他
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/154845
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_外科学_甲状腺外科
通讯作者	Shan, Yunfeng
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, Wenzhou, Peoples R China; 2.Key Lab Diag & Treatment Severe Hepatopancreat Di, Wenzhou, Peoples R China; 3.Third Peoples Hosp Yuhang Dist, Dept Surg, Hangzhou, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 1, Dept Thyroid Surg, Wenzhou, Peoples R China; 5.Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA; 6.Soochow Univ, Affiliated Hosp 1, Dept Gen Surg, Suzhou, Peoples R China
第一作者单位	附属第一医院
推荐引用方式 GB/T 7714	Fu, Haotian,Sun, Hongwei,Kong, Hongru,et al. Discoveries in Pancreatic Physiology and Disease Biology Using Single-Cell RNA Sequencing. 2022.