SIMULTANEOUS DETERMINATION OF TOLBUTAMIDE AND ITS METABOLITE HYDROXYTOLBUTAMIDE IN RAT PLASMA BY LC-MS

doi:10.1080/10826076.2011.621153

科研成果详情

题名	SIMULTANEOUS DETERMINATION OF TOLBUTAMIDE AND ITS METABOLITE HYDROXYTOLBUTAMIDE IN RAT PLASMA BY LC-MS
作者	Zhang, Xiuhua2; Ma, Jianshe3; Hu, Lufeng2; Zhang, Qingwei 4; Wang, Xianqin1; Pan, Jianchun1; Ye, Faqing1
发表日期	2012-12-31
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	ESI hydroxytolbutamide LC-MS pharmacokinetic plasma tolbutamide
其他关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; IONIZATION MASS-SPECTROMETRY ; URINE ; INHIBITION ; DRUG ; OXIDATION ; JUICE
摘要	A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of tolbutamide and its metabolite hydroxytolbutamide in rat plasma was developed and validated. The analytes and internal standard omeprazole were extracted from plasma by liquid-liquid extraction using ethyl acetate, and chromatographically separated on a Zorbax SB-C18 column (2.1 mm x 50 mm, 3.5 mu m) using acetonitrile-0.1% formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode, and selected ion monitoring (SIM) mode used to quantify tolbutamide and its metabolite hydroxytolbutamide. The assay was linear over the range 10-20000 ng/mL for tolbutamide and 5-800 ng/mL for hydroxytolbutamide, with a lower limit of quantification (LLOQ) of 10 ng/mL for tolbutamide and 5 ng/mL for hydroxytolbutamide. Intra- and inter-day precision were less than 12% and the accuracy were in the range 88.8-109.7%. This developed method was successfully used for determination of tolbutamide and metabolite hydroxytolbutamide in rat plasma for pharmacokinetic study.
资助项目	Shanghai Key Laboratory Program of Forensic Medicine (Institute of Forensic Science, Ministry of Justice, China) [KF1101]; Wenzhou Municipal Science and Technology Agency of Zhejiang Province, China [Y20090143]; National Youth Natural Science Foundation of China [81102297]
出版者	TAYLOR & FRANCIS INC
出版地	PHILADELPHIA
ISSN	1082-6076
EISSN	1520-572X
卷号	35 期号:12 页码:1627-1637
DOI	10.1080/10826076.2011.621153
页数	22
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000306117600001
收录类别	SCIE ; EI ; SCOPUS
EI入藏号	20122915248161
EI主题词	Metabolites
URL	查看原文
SCOPUSEID	2-s2.0-84863668579
通讯作者地址	[Ye, Faqing]Wenzhou Med Coll, Analyt & Testing Ctr, Univ Town 325035, Wenzhou, Peoples R China.
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15462
专题	附属第一医院基础医学院（机能实验教学中心）_基础医学实验教学中心_机能实验教学中心（挂靠、校级）
通讯作者	Ye, Faqing
作者单位	1.Wenzhou Med Coll, Analyt & Testing Ctr, Wenzhou, Peoples R China; 2.Wenzhou Med Coll, Affiliated Hosp 1, Wenzhou, Peoples R China; 3.Wenzhou Med Coll, Funct Expt Teaching Ctr, Wenzhou, Peoples R China; 4.Shanghai Inst Pharmaceut Ind, Shanghai, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zhang, Xiuhua,Ma, Jianshe,Hu, Lufeng,et al. SIMULTANEOUS DETERMINATION OF TOLBUTAMIDE AND ITS METABOLITE HYDROXYTOLBUTAMIDE IN RAT PLASMA BY LC-MS[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(12):1627-1637.
APA	Zhang, Xiuhua., Ma, Jianshe., Hu, Lufeng., Zhang, Qingwei., Wang, Xianqin., ... & Ye, Faqing. (2012). SIMULTANEOUS DETERMINATION OF TOLBUTAMIDE AND ITS METABOLITE HYDROXYTOLBUTAMIDE IN RAT PLASMA BY LC-MS. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 35(12), 1627-1637.
MLA	Zhang, Xiuhua,et al."SIMULTANEOUS DETERMINATION OF TOLBUTAMIDE AND ITS METABOLITE HYDROXYTOLBUTAMIDE IN RAT PLASMA BY LC-MS".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 35.12(2012):1627-1637.