Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses

doi:10.1111/evj.13605

科研成果详情

题名	Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses
作者	Xu, Chunyan 1; Wei, Zilin 1; Tan, Feng2; Liu, Ao 1; Yu, Fuchang 1; Zhao, Aiyun 1; Zhang, Longxian 3; Qi, Meng 1; Zhao, Wei2
发表日期	2023-05
发表期刊	EQUINE VETERINARY JOURNAL 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	China Cryptosporidium genetic horse racehorse subtype
其他关键词	EPIDEMIOLOGIC SURVEY ; HORSES ; IDENTIFICATION ; HOMINIS ; PREVALENCE ; GENOTYPE ; PARVUM ; FOALS ; INFECTIONS ; DIVERSITY
摘要	Background Cryptosporidium is a protozoan parasite causing diarrhoea in humans and animals. Although Cryptosporidium has been found in domestic horses (farmed or kept at pasture), there has been only one published study of Cryptosporidium infections in Chinese racehorses, which was restricted to a very small geographical area. Objectives To investigate the presence of Cryptosporidium spp. in the faeces of racehorses in China and to perform molecular characterisation of the parasite. Study design Cross-sectional. Methods A total of 621 fresh faecal samples were collected for DNA extraction from racehorses at 17 equestrian clubs from 12 provinces of China from December 2016 to May 2018. All the DNA were analysed for the presence of Cryptosporidium species/genotypes and subtypes by PCR amplification of the small subunit ribosomal RNA and 60 kDa glycoprotein genes respectively. Results PCR analysis revealed that 11 samples (1.8%) were positive for Cryptosporidium spp. Among them seven samples were identified as C. parvum and four were C. hominis. The C. parvum isolates were identified as subtype IIdA14G1 (n = 4) and IIdA15G1 (n = 3), while all C. hominis isolates were identified as subtype IkA18G1 (n = 4). Main limitations A single faecal sample from each horse was used instead of multiple samples that could improve the detection rates of the parasite. Conclusions Although Cryptosporidium infection rate was relatively low in the investigated racehorses, the presence of zoonotic subtypes IIdA14G and 1IIdA15G1 of C. parvum and IkA18G1 of C. hominis, suggesting that these animals are a potential source of Cryptosporidium in humans.
资助项目	Program for Young and Middle-aged Leading Science, Technology, and Innovation of Xinjiang Production & Construction Group [2018CB034]
出版者	WILEY
出版地	HOBOKEN
ISSN	0425-1644
EISSN	2042-3306
卷号	55 期号:3 页码:474-480
DOI	10.1111/evj.13605
页数	7
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
WOS记录号	WOS:000818899700001
收录类别	SCIE ; PUBMED ; SCOPUS
在线发表日期	2022-06
URL	查看原文
PubMed ID	35680650
SCOPUSEID	2-s2.0-85133263106
通讯作者地址	[Qi, Meng]College of Animal Science and Technology,Tarim University,Xinjiang,Alar,843300,China ; [Zhao, Wei]Department of Parasitology,Wenzhou Medical University,Zhejiang,China
Scopus学科分类	Equine
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/154488
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Qi, Meng; Zhao, Wei
作者单位	1.College of Animal Science and Technology,Tarim University,Xinjiang,China; 2.Department of Parasitology,Wenzhou Medical University,Zhejiang,China; 3.College of Veterinary Medicine,Henan Agricultural University,Henan,Zhengzhou,China
通讯作者单位	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
推荐引用方式 GB/T 7714	Xu, Chunyan,Wei, Zilin,Tan, Feng,et al. Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses[J]. EQUINE VETERINARY JOURNAL,2023,55(3):474-480.
APA	Xu, Chunyan., Wei, Zilin., Tan, Feng., Liu, Ao., Yu, Fuchang., ... & Zhao, Wei. (2023). Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses. EQUINE VETERINARY JOURNAL, 55(3), 474-480.
MLA	Xu, Chunyan,et al."Molecular detection and genetic characteristics of Cryptosporidium spp. in Chinese racehorses".EQUINE VETERINARY JOURNAL 55.3(2023):474-480.