Molecular detection and genetic characterization of Cryptosporidium in kindergarten children in Southern Xinjiang, China

doi:10.1016/j.meegid.2022.105339

科研成果详情

题名	Molecular detection and genetic characterization of Cryptosporidium in kindergarten children in Southern Xinjiang, China
作者	Wang, Tian 1; Wei, Zilin 1; Zhang, Ying 1; Zhang, Qiyuan 1; Zhang, Longxian 2; Yu, Fuchang 1; Qi, Meng 1; Zhao, Wei3
发表日期	2022-09
发表期刊	INFECTION GENETICS AND EVOLUTION 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cryptosporidium Zoonotic transmission Genotyping Children China
其他关键词	DIARRHEA ; HOMINIS ; HUMANS ; PARVUM
摘要	Cryptosporidium is a common cause of diarrhea in children globally. However, there is limited information on the prevalence and genetic characteristics of Cryptosporidium in children in Xinjiang, China. This study aimed to assess the genetic characteristics and epidemiological status of Cryptosporidium in kindergarten children in Southern Xinjiang, China. A total of 609 fecal samples were collected from kindergartners aged 2-6 years from 11 counties in Southern Xinjiang, China. We used nested PCR amplification of the partial SSU rDNA gene to screen samples for Cryptosporidium spp. Isolates containing Cryptosporidium parvum and C. hominis were further subtyped for a gene encoding a 60-kDa glycoprotein (gp60). We used MEGA7 to construct a phylogenetic tree to study the genetic relationship between the gp60 subtypes of these two species via the Maximum Likelihood method based on the Tamura-Nei model. Only 1.3% (8/609) of asymptomatic children were confirmed to be infected with Cryptosporidium, with a 2.0% (6/299) infection rate in boys and 0.6% (2/310) infection rate in girls. Three Cryptosporidium species were identified including C. felis (37.5%; 3/8), C. hominis (37.5%; 3/8), and C. parvum (25.0%; 2/8). Three C. hominis subtypes (IbA9G3, IdA14, and IfA12G1) and two C. parvum subtypes (IIdA14G1 and IIdA15G1) were also found. This study is the first to identify the presence of Cryptosporidium in kindergarten children in Southern Xinjiang, China. The presence of zoonotic C. parvum subtypes IIdA14G1 and IIdA15G1 indicates the possible cross-species transmission of Cryptosporidium between children and animals.
资助项目	National Natural Science Foundation of China [31702227]; Program for Young and Middle- aged Leading Science, Technology, and Innovation of Xinjiang Produc- tion & Construction Corps [2018CB034]; Key Technologies R & D Programme of Xinjiang Production & Construction Corps [2020AB025]
出版者	ELSEVIER
出版地	AMSTERDAM
ISSN	1567-1348
EISSN	1567-7257
卷号	103 页码:105339
DOI	10.1016/j.meegid.2022.105339
页数	6
WOS类目	Infectious Diseases
WOS研究方向	Infectious Diseases
WOS记录号	WOS:000830876800003
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	35840104
SCOPUSEID	2-s2.0-85134537408
通讯作者地址	[Zhao, Wei]School of Basic Medicine,Wenzhou Medical University,Zhejiang Province,Wenzhou,325035,China ; [Qi, Meng]College of Animal Science,Tarim University,Tarim Road 1487, Xinjiang,Alar,843300,China
Scopus学科分类	Microbiology;Ecology, Evolution, Behavior and Systematics;Molecular Biology;Genetics;Microbiology (medical);Infectious Diseases
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/154172
专题	基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学寄生虫学
通讯作者	Qi, Meng; Zhao, Wei
作者单位	1.College of Animal Science and Technology,Tarim University,Xinjiang,Alar,843300,China; 2.College of Veterinary Medicine,Henan Agricultural University,Henan,Zhengzhou,450046,China; 3.Department of Parasitology,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
通讯作者单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Wang, Tian,Wei, Zilin,Zhang, Ying,et al. Molecular detection and genetic characterization of Cryptosporidium in kindergarten children in Southern Xinjiang, China[J]. INFECTION GENETICS AND EVOLUTION,2022,103:105339.
APA	Wang, Tian., Wei, Zilin., Zhang, Ying., Zhang, Qiyuan., Zhang, Longxian., ... & Zhao, Wei. (2022). Molecular detection and genetic characterization of Cryptosporidium in kindergarten children in Southern Xinjiang, China. INFECTION GENETICS AND EVOLUTION, 103, 105339.
MLA	Wang, Tian,et al."Molecular detection and genetic characterization of Cryptosporidium in kindergarten children in Southern Xinjiang, China".INFECTION GENETICS AND EVOLUTION 103(2022):105339.