Expression and effect of CXCL14 in colorectal carcinoma

doi:10.3892/mmr.2014.2343

科研成果详情

题名	Expression and effect of CXCL14 in colorectal carcinoma
作者	Lin, Kezhi 1; Zou, Ruanmin2; Lin, Feng 3; Zheng, Shuang 3; Shen, Xian4; Xue, Xiangyang5
发表日期	2014-09
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	colorectal carcinoma chemokine CXCL14 lentivirus survival analysis
其他关键词	CHEMOKINE RECEPTOR CCR6 ; IN-VIVO ; HEPATOCELLULAR-CARCINOMA ; DENDRITIC CELLS ; IMMUNE-RESPONSE ; BREAST-CANCER ; LIGAND 14 ; TISSUE ; BRAK ; MIGRATION
摘要	Chemokines are important in the proliferation and metastasis of tumors. CXCL14 is a member of the CXCL chemokine family and exhibits various expression patterns in different types of tumor, even those tumors that occur in the same type of tissue. The expression of CXCL14 and its clinical significance in colorectal carcinoma are unclear. In the present study, the expression levels of CXCL14 in colorectal carcinoma and adjacent normal tissues were detected using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. Kaplan-Meier survival curves and the Cox regression model were applied to evaluate the clinical significance of the expression levels of CXCL14 in colorectal carcinoma compared with those in normal tissues. To investigate the effects at a cellular level, a replication-defective lentivirus overexpressing CXCL14 was constructed and transfected into HT29 colorectal carcinoma cells. The effect of CXCL14 on the proliferation of colorectal carcinoma cells and the change in cell cycle distributions were investigated using a cell counting kit-8 assay and flow cytometry, respectively. Results of the current study indicated that the expression levels of CXCL14 mRNA and protein in colorectal carcinoma were markedly reduced compared with levels in normal tissues (P<0.05). The clinical correlation analysis suggested that downregulation of CXCL14 expression in tumors was associated with lymph metastasis, tumor location, and clinicopathological stage (P<0.05). Kaplan-Meier survival. analysis revealed that downregulation of CXCL14 expression was correlated with a poor prognosis (P<0.01). Overexpression of CXCL14 by lentiviral transfection produced an inhibitory effect on cell proliferation by arresting the cell cycle in the G, stage. The data of the current study suggest that CXCL14 may be involved in the development and progression of colorectal carcinoma, and may act directly as a potential cancer suppressor gene. The level of CXCL14 expression may be a valuable adjuvant parameter in predicting the prognosis of colorectal carcinoma and may be a potential therapeutic target.
资助项目	Department of Education, Zhejiang [Y201327980]; Taizhou Science and Technology Bureau [1301ky40]; Zhejiang Science and Technology Bureau [2012C33126, 2012C37080]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	10 期号:3 页码:1561-1568
DOI	10.3892/mmr.2014.2343
页数	8
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000340854500058
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	24938992
SCOPUSEID	2-s2.0-84905447806
通讯作者地址	[Lin, Feng]Department of General Surgery, Taizhou First People's Hospital,No. 218 Hengjie Street, Huang Yan District,China
Scopus学科分类	Biochemistry;Molecular Medicine;Molecular Biology;Genetics;Oncology;Cancer Research
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15348
专题	基础医学院（机能实验教学中心）附属第一医院_胃肠外科检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Lin, Feng
作者单位	1.Experimental Teaching Center, Wenzhou Medical University,China; 2.Department of Obstetrics and Gynecology, First Affiliated Hospital, Wenzhou Medical University,China; 3.Department of General Surgery, Taizhou First People's Hospital,No. 218 Hengjie Street, Huang Yan District,China; 4.Department of Gastrointestinal Surgery, First Affiliated Hospital, Wenzhou Medical University,China; 5.Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Wenzhou Medical University,China
第一作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Lin, Kezhi,Zou, Ruanmin,Lin, Feng,et al. Expression and effect of CXCL14 in colorectal carcinoma[J]. MOLECULAR MEDICINE REPORTS,2014,10(3):1561-1568.
APA	Lin, Kezhi, Zou, Ruanmin, Lin, Feng, Zheng, Shuang, Shen, Xian, & Xue, Xiangyang. (2014). Expression and effect of CXCL14 in colorectal carcinoma. MOLECULAR MEDICINE REPORTS, 10(3), 1561-1568.
MLA	Lin, Kezhi,et al."Expression and effect of CXCL14 in colorectal carcinoma".MOLECULAR MEDICINE REPORTS 10.3(2014):1561-1568.