Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo

doi:10.3892/etm.2021.10018

科研成果详情

题名	Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo
作者	Sun, Miaoxuan1; Sheng, Yi 2; Zhu, Yanyan1
发表日期	2021-06
发表期刊	EXPERIMENTAL AND THERAPEUTIC MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	ginkgolide B neuroinflammation inflammatory response microglial cells BV2 cells
摘要	Ginkgolide B (GB) is widely used in the treatment of neurological diseases and exerts anti-inflammatory and neuroprotective effects. Microglia serve an important role in central nervous inflammation. The present study investigated the effect of GB on central nervous inflammation in vivo and in vitro. BV2 cells were activated with lipopolysaccharide (LPS) to establish a cellular model of neuroinflammation. Cell viability was determined using the Cell Counting Kit-8 assay and the secreted levels of TNF-alpha, IL-1 and IL-6 were measured using ELISAs. The levels of nitric oxide (NO) was assessed using Griess assays. In addition, the mRNA and protein expression levels of inducible NO synthase and cyclooxygenase-2 (COX-2) were detected using reverse transcription-quantitative PCR and western blot analyses, respectively. Transwell assays were carried out to evaluate the cell migratory ability. For the in vivo studies, an LPS-induced neuroinflammation model was established in C57 mice. Western blot analysis and immunohistochemistry were performed to detect the expression of the microglial marker allograft inflammatory factor 1 in the hippocampal dentate gyrus and striatum. The expression levels of TNF-alpha, IL-1 and IL-6 in the hippocampal dentate gyrus and striatum were assessed using western blot analysis. The results revealed that GB reduced the inflammatory response and migration of LPS-induced BV2 cells. Furthermore, GB attenuated the activation of BV2 cells of the hippocampal dentate gyrus and striatum in the LPS-induced mice with neuroinflammation. Taken together, the findings of the present study demonstrated that GB alleviated the inflammatory response and attenuated the activation of LPS-induced BV2 cells in vitro and in vivo.
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1792-0981
EISSN	1792-1015
卷号	21 期号:6 页码:586
DOI	10.3892/etm.2021.10018
页数	8
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000638206900001
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	33850558
PMC记录号	PMC8027720
通讯作者地址	[Sun, Miaoxuan]Wenzhou Med Univ, Affiliated Hosp 1, Dept Rehabil, Pk Rd,2 Fuxue Lane, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15299
专题	附属第一医院_康复科
通讯作者	Sun, Miaoxuan
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 1, Dept Rehabil, Pk Rd,2 Fuxue Lane, Wenzhou 325000, Zhejiang, Peoples R China; 2.Ouhai Dist Hosp Integrated Tradit Chinese & Weste, Dept Anesthesia, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院_康复科
通讯作者单位	附属第一医院_康复科
第一作者的第一单位	附属第一医院_康复科
推荐引用方式 GB/T 7714	Sun, Miaoxuan,Sheng, Yi,Zhu, Yanyan. Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2021,21(6):586.
APA	Sun, Miaoxuan, Sheng, Yi, & Zhu, Yanyan. (2021). Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 21(6), 586.
MLA	Sun, Miaoxuan,et al."Ginkgolide B alleviates the inflammatory response and attenuates the activation of LPS-induced BV2 cells in vitro and in vivo".EXPERIMENTAL AND THERAPEUTIC MEDICINE 21.6(2021):586.